Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1628 and Pf1N1B4_1629 are separated by 258 nucleotides Pf1N1B4_1629 and Pf1N1B4_1630 are separated by 77 nucleotides Pf1N1B4_1630 and Pf1N1B4_1631 overlap by 4 nucleotides
Pf1N1B4_1628 - MFS permease, at 1,871,107 to 1,872,771
_1628
Pf1N1B4_1629 - FIG00958480: hypothetical protein, at 1,873,030 to 1,873,434
_1629
Pf1N1B4_1630 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 1,873,512 to 1,875,746
_1630
Pf1N1B4_1631 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 1,875,743 to 1,876,810
_1631
Position (kb)
1873
1874
1875
1876 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1872.627 kb on - strand at 1872.765 kb on + strand at 1872.765 kb on + strand at 1872.765 kb on + strand at 1872.787 kb on + strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.788 kb on - strand at 1872.790 kb on + strand at 1872.790 kb on + strand at 1872.790 kb on + strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.791 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.794 kb on - strand at 1872.827 kb on - strand at 1872.852 kb on - strand at 1872.852 kb on - strand at 1872.947 kb on + strand at 1872.948 kb on - strand at 1872.961 kb on - strand at 1872.961 kb on - strand at 1872.978 kb on - strand at 1872.993 kb on + strand at 1873.067 kb on - strand at 1873.067 kb on - strand at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.259 kb on + strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.260 kb on - strand, within Pf1N1B4_1629 at 1873.465 kb on - strand at 1873.466 kb on + strand at 1873.466 kb on + strand at 1873.466 kb on + strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.467 kb on - strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.497 kb on + strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.498 kb on - strand at 1873.543 kb on + strand at 1873.543 kb on + strand at 1873.544 kb on - strand at 1873.544 kb on - strand at 1873.544 kb on - strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.614 kb on + strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.615 kb on - strand at 1873.616 kb on + strand at 1873.617 kb on - strand at 1873.617 kb on - strand at 1874.062 kb on - strand, within Pf1N1B4_1630 at 1874.062 kb on - strand, within Pf1N1B4_1630 at 1874.245 kb on - strand, within Pf1N1B4_1630 at 1874.245 kb on - strand, within Pf1N1B4_1630 at 1874.710 kb on - strand, within Pf1N1B4_1630 at 1874.710 kb on - strand, within Pf1N1B4_1630 at 1875.010 kb on - strand, within Pf1N1B4_1630 at 1875.010 kb on - strand, within Pf1N1B4_1630 at 1875.036 kb on - strand, within Pf1N1B4_1630 at 1875.247 kb on + strand, within Pf1N1B4_1630 at 1875.248 kb on - strand, within Pf1N1B4_1630 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.893 kb on + strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.894 kb on - strand, within Pf1N1B4_1631 at 1875.989 kb on + strand at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1875.989 kb on + strand, within Pf1N1B4_1631 at 1876.255 kb on + strand, within Pf1N1B4_1631 at 1876.262 kb on + strand, within Pf1N1B4_1631 at 1876.262 kb on + strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.281 kb on - strand, within Pf1N1B4_1631 at 1876.642 kb on - strand, within Pf1N1B4_1631 at 1876.642 kb on - strand, within Pf1N1B4_1631 at 1876.712 kb on + strand at 1876.712 kb on + strand at 1876.712 kb on + strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.713 kb on - strand at 1876.714 kb on + strand at 1876.715 kb on - strand at 1876.715 kb on - strand at 1876.715 kb on - strand at 1876.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,872,627 - +0.4 1,872,765 + -1.8 1,872,765 + +0.4 1,872,765 + +1.8 1,872,787 + -1.2 1,872,788 - -1.0 1,872,788 - -0.1 1,872,788 - -1.0 1,872,788 - -0.5 1,872,788 - +0.2 1,872,788 - -1.1 1,872,788 - -0.6 1,872,788 - -1.1 1,872,788 - -0.8 1,872,788 - -0.4 1,872,788 - -0.1 1,872,788 - -0.5 1,872,788 - +0.5 1,872,788 - -0.9 1,872,788 - -0.9 1,872,788 - -0.9 1,872,788 - -0.8 1,872,788 - +0.8 1,872,788 - +2.0 1,872,788 - +0.1 1,872,788 - -0.8 1,872,788 - -0.4 1,872,788 - -0.8 1,872,790 + +0.5 1,872,790 + -0.2 1,872,790 + +0.4 1,872,791 - +0.5 1,872,791 - +0.1 1,872,791 - +0.1 1,872,791 - +0.0 1,872,791 - -0.2 1,872,791 - +0.5 1,872,791 - -0.0 1,872,791 - +0.4 1,872,791 - -1.3 1,872,791 - -0.1 1,872,791 - +1.4 1,872,791 - -0.4 1,872,791 - +2.1 1,872,791 - -1.4 1,872,791 - +0.1 1,872,791 - -0.9 1,872,791 - -0.1 1,872,791 - +0.7 1,872,791 - -0.2 1,872,791 - +0.1 1,872,791 - -0.0 1,872,791 - +0.1 1,872,791 - -0.7 1,872,791 - -0.1 1,872,791 - -0.2 1,872,791 - +2.0 1,872,794 - -2.0 1,872,794 - +0.2 1,872,794 - -0.0 1,872,794 - +0.7 1,872,794 - +0.1 1,872,794 - +0.5 1,872,794 - +1.4 1,872,794 - +0.3 1,872,794 - +0.2 1,872,794 - +0.7 1,872,794 - -0.0 1,872,794 - +1.1 1,872,794 - -0.3 1,872,794 - -0.7 1,872,794 - -1.4 1,872,827 - -0.7 1,872,852 - +1.4 1,872,852 - -2.2 1,872,947 + -2.4 1,872,948 - -0.6 1,872,961 - -0.3 1,872,961 - +1.4 1,872,978 - +0.8 1,872,993 + -0.2 1,873,067 - +2.4 1,873,067 - +0.1 1,873,259 + Pf1N1B4_1629 0.57 +0.1 1,873,259 + Pf1N1B4_1629 0.57 +0.6 1,873,259 + Pf1N1B4_1629 0.57 -0.2 1,873,259 + Pf1N1B4_1629 0.57 +1.0 1,873,259 + Pf1N1B4_1629 0.57 -0.9 1,873,260 - Pf1N1B4_1629 0.57 +0.4 1,873,260 - Pf1N1B4_1629 0.57 -0.0 1,873,260 - Pf1N1B4_1629 0.57 +1.8 1,873,260 - Pf1N1B4_1629 0.57 +1.1 1,873,260 - Pf1N1B4_1629 0.57 +0.9 1,873,260 - Pf1N1B4_1629 0.57 +0.1 1,873,465 - -0.5 1,873,466 + -0.9 1,873,466 + -0.2 1,873,466 + +0.4 1,873,467 - +0.8 1,873,467 - -1.2 1,873,467 - +0.1 1,873,467 - -0.4 1,873,467 - -0.7 1,873,467 - -0.4 1,873,497 + +0.6 1,873,497 + -1.9 1,873,497 + -0.9 1,873,497 + -1.6 1,873,497 + +0.4 1,873,497 + +1.7 1,873,497 + -0.6 1,873,497 + -0.5 1,873,497 + -0.6 1,873,498 - -0.5 1,873,498 - +0.4 1,873,498 - +0.4 1,873,498 - +0.1 1,873,498 - +1.4 1,873,498 - -1.8 1,873,543 + -1.8 1,873,543 + +0.4 1,873,544 - -0.2 1,873,544 - +1.2 1,873,544 - -1.7 1,873,614 + -0.2 1,873,614 + -0.5 1,873,614 + -0.2 1,873,614 + +1.1 1,873,615 - -0.0 1,873,615 - -0.7 1,873,615 - +0.4 1,873,615 - -0.4 1,873,616 + -0.7 1,873,617 - +0.1 1,873,617 - -1.2 1,874,062 - Pf1N1B4_1630 0.25 -1.9 1,874,062 - Pf1N1B4_1630 0.25 -1.6 1,874,245 - Pf1N1B4_1630 0.33 -0.2 1,874,245 - Pf1N1B4_1630 0.33 -0.6 1,874,710 - Pf1N1B4_1630 0.54 -0.9 1,874,710 - Pf1N1B4_1630 0.54 -1.6 1,875,010 - Pf1N1B4_1630 0.67 -1.1 1,875,010 - Pf1N1B4_1630 0.67 +0.8 1,875,036 - Pf1N1B4_1630 0.68 -0.2 1,875,247 + Pf1N1B4_1630 0.78 -1.4 1,875,248 - Pf1N1B4_1630 0.78 -1.0 1,875,893 + Pf1N1B4_1631 0.14 +0.4 1,875,893 + Pf1N1B4_1631 0.14 -2.1 1,875,893 + Pf1N1B4_1631 0.14 +0.8 1,875,893 + Pf1N1B4_1631 0.14 +0.9 1,875,893 + Pf1N1B4_1631 0.14 -1.3 1,875,893 + Pf1N1B4_1631 0.14 -0.2 1,875,894 - Pf1N1B4_1631 0.14 +1.4 1,875,894 - Pf1N1B4_1631 0.14 -0.5 1,875,894 - Pf1N1B4_1631 0.14 +0.2 1,875,989 + +0.4 1,875,989 + Pf1N1B4_1631 0.23 +1.6 1,875,989 + Pf1N1B4_1631 0.23 +0.6 1,875,989 + Pf1N1B4_1631 0.23 -0.4 1,875,989 + Pf1N1B4_1631 0.23 +0.1 1,875,989 + Pf1N1B4_1631 0.23 -0.2 1,876,255 + Pf1N1B4_1631 0.48 -2.4 1,876,262 + Pf1N1B4_1631 0.49 +0.4 1,876,262 + Pf1N1B4_1631 0.49 -2.1 1,876,281 - Pf1N1B4_1631 0.50 +1.8 1,876,281 - Pf1N1B4_1631 0.50 -1.5 1,876,281 - Pf1N1B4_1631 0.50 +1.1 1,876,642 - Pf1N1B4_1631 0.84 -1.9 1,876,642 - Pf1N1B4_1631 0.84 -0.9 1,876,712 + +1.4 1,876,712 + +0.5 1,876,712 + -0.1 1,876,713 - +0.6 1,876,713 - -0.6 1,876,713 - +1.2 1,876,713 - -1.5 1,876,714 + +0.5 1,876,715 - +1.8 1,876,715 - +2.0 1,876,715 - -0.8 1,876,715 - +0.1
Or see this region's nucleotide sequence