Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1606 and Pf1N1B4_1607 are separated by 353 nucleotides Pf1N1B4_1607 and Pf1N1B4_1608 are separated by 73 nucleotides Pf1N1B4_1608 and Pf1N1B4_1609 overlap by 4 nucleotides
Pf1N1B4_1606 - Glutamine synthetase type I (EC 6.3.1.2), at 1,851,177 to 1,852,583
_1606
Pf1N1B4_1607 - hypothetical protein, at 1,852,937 to 1,853,185
_1607
Pf1N1B4_1608 - FIG00956533: hypothetical protein, at 1,853,259 to 1,853,726
_1608
Pf1N1B4_1609 - FIG00953806: hypothetical protein, at 1,853,723 to 1,854,322
_1609
Position (kb)
1853
1854 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1852.645 kb on - strand at 1852.645 kb on - strand at 1852.687 kb on + strand at 1852.687 kb on + strand at 1852.688 kb on - strand at 1852.688 kb on - strand at 1852.739 kb on + strand at 1852.740 kb on - strand at 1852.740 kb on - strand at 1852.788 kb on + strand at 1852.789 kb on - strand at 1852.789 kb on - strand at 1852.891 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.062 kb on + strand, within Pf1N1B4_1607 at 1853.063 kb on - strand, within Pf1N1B4_1607 at 1853.098 kb on + strand, within Pf1N1B4_1607 at 1853.099 kb on - strand, within Pf1N1B4_1607 at 1853.140 kb on + strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.167 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.184 kb on - strand at 1853.184 kb on - strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.271 kb on + strand at 1853.271 kb on + strand at 1853.272 kb on - strand at 1853.272 kb on - strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.515 kb on + strand, within Pf1N1B4_1608 at 1853.560 kb on + strand, within Pf1N1B4_1608 at 1853.561 kb on - strand, within Pf1N1B4_1608 at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.705 kb on - strand at 1853.717 kb on + strand at 1853.717 kb on + strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.718 kb on - strand at 1853.782 kb on + strand at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.836 kb on + strand, within Pf1N1B4_1609 at 1853.983 kb on + strand at 1854.341 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.481 kb on + strand at 1854.482 kb on - strand at 1854.531 kb on + strand at 1854.532 kb on - strand at 1854.534 kb on - strand at 1854.534 kb on - strand at 1854.544 kb on + strand at 1854.557 kb on - strand at 1854.557 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,852,645 - -0.8 1,852,645 - +0.2 1,852,687 + -0.0 1,852,687 + +0.4 1,852,688 - +0.6 1,852,688 - +0.7 1,852,739 + -0.5 1,852,740 - +1.4 1,852,740 - -1.8 1,852,788 + -1.4 1,852,789 - -1.9 1,852,789 - -0.1 1,852,891 + -2.1 1,852,901 + -0.2 1,852,901 + +0.4 1,852,901 + +0.2 1,852,902 - -0.3 1,852,902 - -0.6 1,852,902 - +0.8 1,852,902 - +0.1 1,852,902 - +0.8 1,852,902 - -1.6 1,852,902 - -0.4 1,852,902 - -1.6 1,852,902 - +0.5 1,853,006 + Pf1N1B4_1607 0.28 +0.5 1,853,006 + +0.4 1,853,006 + Pf1N1B4_1607 0.28 +1.6 1,853,006 + Pf1N1B4_1607 0.28 -0.5 1,853,006 + Pf1N1B4_1607 0.28 +2.1 1,853,006 + Pf1N1B4_1607 0.28 +1.0 1,853,006 + Pf1N1B4_1607 0.28 -0.6 1,853,006 + Pf1N1B4_1607 0.28 -1.9 1,853,006 + Pf1N1B4_1607 0.28 -0.6 1,853,006 + Pf1N1B4_1607 0.28 +0.4 1,853,006 + Pf1N1B4_1607 0.28 +0.4 1,853,006 + Pf1N1B4_1607 0.28 -0.9 1,853,006 + Pf1N1B4_1607 0.28 +2.6 1,853,006 + Pf1N1B4_1607 0.28 -0.3 1,853,007 - Pf1N1B4_1607 0.28 -0.3 1,853,007 - Pf1N1B4_1607 0.28 +0.3 1,853,007 - Pf1N1B4_1607 0.28 -1.4 1,853,007 - Pf1N1B4_1607 0.28 -0.1 1,853,007 - Pf1N1B4_1607 0.28 +0.6 1,853,007 - Pf1N1B4_1607 0.28 -1.6 1,853,007 - Pf1N1B4_1607 0.28 -1.9 1,853,007 - Pf1N1B4_1607 0.28 +0.1 1,853,007 - Pf1N1B4_1607 0.28 -0.1 1,853,007 - Pf1N1B4_1607 0.28 +0.3 1,853,007 - Pf1N1B4_1607 0.28 -2.5 1,853,007 - Pf1N1B4_1607 0.28 -0.2 1,853,007 - Pf1N1B4_1607 0.28 +0.4 1,853,007 - Pf1N1B4_1607 0.28 -0.6 1,853,007 - Pf1N1B4_1607 0.28 +0.2 1,853,007 - Pf1N1B4_1607 0.28 +0.2 1,853,007 - Pf1N1B4_1607 0.28 +0.7 1,853,007 - Pf1N1B4_1607 0.28 +0.9 1,853,007 - Pf1N1B4_1607 0.28 -0.4 1,853,007 - Pf1N1B4_1607 0.28 +0.2 1,853,007 - Pf1N1B4_1607 0.28 +0.6 1,853,007 - Pf1N1B4_1607 0.28 +0.6 1,853,007 - Pf1N1B4_1607 0.28 -1.8 1,853,007 - Pf1N1B4_1607 0.28 -0.2 1,853,009 - Pf1N1B4_1607 0.29 -0.2 1,853,009 - Pf1N1B4_1607 0.29 -0.1 1,853,009 - Pf1N1B4_1607 0.29 +2.6 1,853,009 - Pf1N1B4_1607 0.29 -0.0 1,853,009 - Pf1N1B4_1607 0.29 +0.2 1,853,026 - Pf1N1B4_1607 0.36 +0.4 1,853,026 - Pf1N1B4_1607 0.36 +0.4 1,853,026 - +0.4 1,853,026 - Pf1N1B4_1607 0.36 -1.4 1,853,062 + Pf1N1B4_1607 0.50 +0.8 1,853,063 - Pf1N1B4_1607 0.51 +1.1 1,853,098 + Pf1N1B4_1607 0.65 +0.8 1,853,099 - Pf1N1B4_1607 0.65 -0.6 1,853,140 + Pf1N1B4_1607 0.82 -0.1 1,853,141 - Pf1N1B4_1607 0.82 -0.1 1,853,141 - Pf1N1B4_1607 0.82 -1.6 1,853,141 - Pf1N1B4_1607 0.82 +0.9 1,853,141 - Pf1N1B4_1607 0.82 +1.9 1,853,141 - Pf1N1B4_1607 0.82 +0.4 1,853,150 + Pf1N1B4_1607 0.86 +0.2 1,853,150 + Pf1N1B4_1607 0.86 -0.5 1,853,150 + Pf1N1B4_1607 0.86 +1.6 1,853,150 + Pf1N1B4_1607 0.86 -0.0 1,853,151 - Pf1N1B4_1607 0.86 +0.1 1,853,151 - Pf1N1B4_1607 0.86 +1.1 1,853,151 - Pf1N1B4_1607 0.86 -0.5 1,853,151 - Pf1N1B4_1607 0.86 +0.4 1,853,151 - Pf1N1B4_1607 0.86 +0.1 1,853,151 - Pf1N1B4_1607 0.86 +1.4 1,853,151 - Pf1N1B4_1607 0.86 +0.6 1,853,151 - Pf1N1B4_1607 0.86 -0.0 1,853,151 - Pf1N1B4_1607 0.86 -1.5 1,853,167 + -0.2 1,853,183 + -0.8 1,853,183 + +1.4 1,853,183 + -0.3 1,853,184 - +0.1 1,853,184 - +1.0 1,853,224 + +0.4 1,853,224 + +1.0 1,853,224 + +1.0 1,853,225 - -1.8 1,853,225 - -1.2 1,853,225 - -0.7 1,853,227 + -0.1 1,853,227 + +0.6 1,853,227 + -0.4 1,853,227 + -0.4 1,853,227 + -0.4 1,853,227 + -0.6 1,853,228 - +0.4 1,853,228 - -2.1 1,853,228 - +1.1 1,853,228 - +1.0 1,853,228 - -0.4 1,853,228 - -0.9 1,853,228 - +1.8 1,853,228 - +1.4 1,853,228 - -0.9 1,853,228 - -0.2 1,853,228 - -1.9 1,853,228 - -0.6 1,853,228 - -0.4 1,853,233 + -1.0 1,853,233 + -0.0 1,853,233 + -1.9 1,853,233 + +0.2 1,853,234 - +1.4 1,853,234 - -0.0 1,853,234 - +0.1 1,853,271 + -0.2 1,853,271 + +0.1 1,853,272 - -0.2 1,853,272 - +0.6 1,853,277 + -0.9 1,853,277 + -0.6 1,853,277 + +2.5 1,853,277 + -1.2 1,853,277 + +0.5 1,853,277 + -2.5 1,853,277 + -1.3 1,853,277 + +0.7 1,853,277 + +0.8 1,853,277 + -1.6 1,853,278 - -0.1 1,853,278 - +1.4 1,853,278 - +1.4 1,853,278 - -0.1 1,853,278 - -1.6 1,853,278 - -0.6 1,853,278 - -0.4 1,853,278 - +0.1 1,853,278 - -0.2 1,853,515 + Pf1N1B4_1608 0.55 +0.4 1,853,560 + Pf1N1B4_1608 0.64 -1.1 1,853,561 - Pf1N1B4_1608 0.65 -2.3 1,853,704 + +1.0 1,853,704 + -0.3 1,853,704 + -1.3 1,853,705 - -1.1 1,853,705 - -0.5 1,853,705 - -1.9 1,853,705 - +0.2 1,853,705 - +0.4 1,853,705 - -0.6 1,853,717 + +0.1 1,853,717 + -0.2 1,853,718 - -0.9 1,853,718 - -0.0 1,853,718 - -0.4 1,853,718 - -0.9 1,853,718 - -0.9 1,853,718 - +0.4 1,853,782 + -0.5 1,853,783 - Pf1N1B4_1609 0.10 +0.8 1,853,783 - Pf1N1B4_1609 0.10 -1.4 1,853,786 + Pf1N1B4_1609 0.10 +0.8 1,853,786 + Pf1N1B4_1609 0.10 -3.0 1,853,786 + Pf1N1B4_1609 0.10 -0.0 1,853,787 - Pf1N1B4_1609 0.11 +0.6 1,853,787 - Pf1N1B4_1609 0.11 -1.0 1,853,787 - Pf1N1B4_1609 0.11 -0.6 1,853,787 - Pf1N1B4_1609 0.11 -1.0 1,853,787 - Pf1N1B4_1609 0.11 -1.3 1,853,836 + Pf1N1B4_1609 0.19 +0.3 1,853,983 + -1.2 1,854,341 - +0.8 1,854,348 - +0.4 1,854,348 - +1.1 1,854,348 - -0.2 1,854,348 - +0.8 1,854,348 - +0.4 1,854,348 - +1.4 1,854,348 - -1.0 1,854,348 - +2.4 1,854,348 - -0.4 1,854,348 - +0.8 1,854,348 - -0.8 1,854,481 + +0.4 1,854,482 - +0.8 1,854,531 + -0.2 1,854,532 - -0.4 1,854,534 - -0.3 1,854,534 - -0.4 1,854,544 + +1.4 1,854,557 - +0.8 1,854,557 - +0.4 1,854,589 - +1.8 1,854,589 - +0.0 1,854,589 - +1.0 1,854,589 - +0.5 1,854,648 - -0.6
Or see this region's nucleotide sequence