Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1421 and Pf1N1B4_1422 are separated by 212 nucleotides Pf1N1B4_1422 and Pf1N1B4_1423 are separated by 3 nucleotides Pf1N1B4_1423 and Pf1N1B4_1424 overlap by 1 nucleotides Pf1N1B4_1424 and Pf1N1B4_1425 are separated by 300 nucleotides
Pf1N1B4_1421 - Ribosome small subunit-stimulated GTPase EngC, at 1,629,995 to 1,631,026
_1421
Pf1N1B4_1422 - hypothetical protein, at 1,631,239 to 1,631,646
_1422
Pf1N1B4_1423 - Peptidase C14, caspase catalytic subunit p20, at 1,631,650 to 1,632,609
_1423
Pf1N1B4_1424 - hypothetical protein, at 1,632,609 to 1,633,094
_1424
Pf1N1B4_1425 - hypothetical protein, at 1,633,395 to 1,634,066
_1425
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1631.021 kb on + strand at 1631.021 kb on + strand at 1631.022 kb on - strand at 1631.024 kb on + strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.121 kb on - strand at 1631.144 kb on - strand at 1631.144 kb on - strand at 1631.194 kb on + strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.248 kb on + strand at 1631.248 kb on + strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.467 kb on + strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.676 kb on + strand at 1631.677 kb on - strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.680 kb on - strand at 1631.680 kb on - strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1632.002 kb on + strand, within Pf1N1B4_1423 at 1632.024 kb on + strand, within Pf1N1B4_1423 at 1632.025 kb on - strand, within Pf1N1B4_1423 at 1632.179 kb on + strand, within Pf1N1B4_1423 at 1632.179 kb on + strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.241 kb on + strand, within Pf1N1B4_1423 at 1632.242 kb on - strand, within Pf1N1B4_1423 at 1632.242 kb on - strand, within Pf1N1B4_1423 at 1632.341 kb on + strand, within Pf1N1B4_1423 at 1632.342 kb on - strand, within Pf1N1B4_1423 at 1632.415 kb on + strand, within Pf1N1B4_1423 at 1632.416 kb on - strand, within Pf1N1B4_1423 at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.526 kb on + strand at 1632.526 kb on + strand at 1632.527 kb on - strand at 1632.527 kb on - strand at 1632.527 kb on - strand at 1632.574 kb on + strand at 1632.575 kb on - strand at 1632.575 kb on - strand at 1632.575 kb on - strand at 1632.578 kb on - strand at 1632.620 kb on + strand at 1632.620 kb on + strand at 1632.620 kb on + strand at 1632.621 kb on - strand at 1632.621 kb on - strand at 1632.627 kb on + strand at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.866 kb on - strand, within Pf1N1B4_1424 at 1632.904 kb on - strand, within Pf1N1B4_1424 at 1632.911 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.033 kb on - strand, within Pf1N1B4_1424 at 1633.033 kb on - strand, within Pf1N1B4_1424 at 1633.135 kb on + strand at 1633.135 kb on + strand at 1633.136 kb on - strand at 1633.136 kb on - strand at 1633.159 kb on + strand at 1633.159 kb on + strand at 1633.160 kb on - strand at 1633.212 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.330 kb on + strand at 1633.366 kb on + strand at 1633.370 kb on - strand at 1633.553 kb on + strand, within Pf1N1B4_1425 at 1633.554 kb on - strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,631,021 + -0.5 1,631,021 + -2.3 1,631,022 - -0.8 1,631,024 + +0.7 1,631,025 - +0.7 1,631,025 - +1.3 1,631,025 - +0.6 1,631,075 - -0.7 1,631,075 - +0.6 1,631,075 - +0.3 1,631,075 - -0.3 1,631,075 - -2.0 1,631,075 - +0.7 1,631,083 - +0.1 1,631,083 - +0.2 1,631,083 - +0.7 1,631,121 - +1.0 1,631,144 - +0.3 1,631,144 - -0.3 1,631,194 + -1.0 1,631,195 - -0.1 1,631,195 - +0.1 1,631,195 - +0.2 1,631,195 - +0.8 1,631,195 - -1.0 1,631,195 - +1.7 1,631,195 - +1.3 1,631,195 - +1.3 1,631,248 + +0.3 1,631,248 + +0.7 1,631,249 - -1.3 1,631,249 - -1.0 1,631,249 - -0.9 1,631,458 + Pf1N1B4_1422 0.54 +0.7 1,631,458 + Pf1N1B4_1422 0.54 -0.7 1,631,458 + Pf1N1B4_1422 0.54 +1.9 1,631,458 + Pf1N1B4_1422 0.54 +1.3 1,631,458 + Pf1N1B4_1422 0.54 -0.8 1,631,458 + Pf1N1B4_1422 0.54 +0.3 1,631,458 + Pf1N1B4_1422 0.54 -1.3 1,631,459 - Pf1N1B4_1422 0.54 +1.1 1,631,459 - Pf1N1B4_1422 0.54 +2.3 1,631,459 - Pf1N1B4_1422 0.54 -0.7 1,631,459 - Pf1N1B4_1422 0.54 +0.3 1,631,467 + Pf1N1B4_1422 0.56 +0.6 1,631,468 - Pf1N1B4_1422 0.56 +0.0 1,631,468 - Pf1N1B4_1422 0.56 -1.3 1,631,468 - Pf1N1B4_1422 0.56 +0.0 1,631,676 + +0.6 1,631,677 - -0.4 1,631,679 + -1.7 1,631,679 + +0.3 1,631,679 + -0.7 1,631,679 + -1.1 1,631,680 - -0.3 1,631,680 - +2.9 1,631,683 + -0.7 1,631,683 + +2.6 1,631,683 + -0.2 1,631,683 + +0.8 1,631,683 + -2.3 1,631,683 + -1.0 1,631,683 + +0.0 1,631,683 + -0.7 1,631,683 + +0.3 1,631,683 + +0.4 1,631,683 + -0.3 1,631,684 - -0.3 1,631,684 - -1.0 1,631,684 - -1.3 1,631,878 + Pf1N1B4_1423 0.24 +2.9 1,631,878 + Pf1N1B4_1423 0.24 -0.5 1,631,878 + Pf1N1B4_1423 0.24 +2.6 1,631,879 - Pf1N1B4_1423 0.24 -0.4 1,631,879 - Pf1N1B4_1423 0.24 -1.0 1,631,879 - Pf1N1B4_1423 0.24 +1.8 1,631,942 - Pf1N1B4_1423 0.30 +1.5 1,631,942 - Pf1N1B4_1423 0.30 +0.5 1,631,942 - Pf1N1B4_1423 0.30 +0.7 1,632,002 + Pf1N1B4_1423 0.37 -0.5 1,632,024 + Pf1N1B4_1423 0.39 -1.5 1,632,025 - Pf1N1B4_1423 0.39 +1.0 1,632,179 + Pf1N1B4_1423 0.55 +0.4 1,632,179 + Pf1N1B4_1423 0.55 -0.7 1,632,213 - Pf1N1B4_1423 0.59 +0.5 1,632,213 - Pf1N1B4_1423 0.59 +1.1 1,632,213 - Pf1N1B4_1423 0.59 -0.7 1,632,213 - Pf1N1B4_1423 0.59 +0.9 1,632,241 + Pf1N1B4_1423 0.62 +0.6 1,632,242 - Pf1N1B4_1423 0.62 +0.3 1,632,242 - Pf1N1B4_1423 0.62 -0.5 1,632,341 + Pf1N1B4_1423 0.72 +1.3 1,632,342 - Pf1N1B4_1423 0.72 -0.7 1,632,415 + Pf1N1B4_1423 0.80 -1.5 1,632,416 - Pf1N1B4_1423 0.80 -0.2 1,632,523 + -0.3 1,632,523 + -1.0 1,632,523 + +0.6 1,632,523 + -0.6 1,632,523 + -0.3 1,632,523 + -1.3 1,632,523 + +0.3 1,632,524 - +0.1 1,632,524 - -1.0 1,632,524 - -0.3 1,632,524 - -0.3 1,632,524 - +0.3 1,632,524 - +0.3 1,632,524 - -1.3 1,632,524 - +1.6 1,632,524 - +0.0 1,632,524 - +0.3 1,632,524 - -1.0 1,632,524 - -0.3 1,632,524 - -1.0 1,632,524 - +1.7 1,632,524 - -0.8 1,632,524 - -2.1 1,632,526 + -1.8 1,632,526 + +2.3 1,632,527 - +0.0 1,632,527 - -0.5 1,632,527 - -0.5 1,632,574 + -0.5 1,632,575 - -0.3 1,632,575 - +0.0 1,632,575 - -1.7 1,632,578 - -1.5 1,632,620 + -1.6 1,632,620 + +0.4 1,632,620 + -0.2 1,632,621 - -0.7 1,632,621 - -0.4 1,632,627 + -0.1 1,632,696 + Pf1N1B4_1424 0.18 -1.8 1,632,696 + Pf1N1B4_1424 0.18 +0.5 1,632,696 + Pf1N1B4_1424 0.18 -1.5 1,632,697 - Pf1N1B4_1424 0.18 +1.1 1,632,697 - Pf1N1B4_1424 0.18 -0.5 1,632,697 - Pf1N1B4_1424 0.18 +0.0 1,632,697 - Pf1N1B4_1424 0.18 -0.5 1,632,697 - Pf1N1B4_1424 0.18 +1.9 1,632,866 - Pf1N1B4_1424 0.53 +1.3 1,632,904 - Pf1N1B4_1424 0.61 +0.3 1,632,911 + Pf1N1B4_1424 0.62 +0.1 1,633,032 + Pf1N1B4_1424 0.87 +0.6 1,633,032 + Pf1N1B4_1424 0.87 -0.6 1,633,032 + Pf1N1B4_1424 0.87 -0.1 1,633,033 - Pf1N1B4_1424 0.87 -0.8 1,633,033 - Pf1N1B4_1424 0.87 -0.9 1,633,135 + -0.7 1,633,135 + -1.7 1,633,136 - -1.3 1,633,136 - -0.6 1,633,159 + -0.7 1,633,159 + -1.1 1,633,160 - +0.3 1,633,212 + +0.4 1,633,318 + +2.3 1,633,318 + +0.1 1,633,318 + -0.5 1,633,318 + +0.3 1,633,319 - +0.7 1,633,319 - +0.3 1,633,319 - -0.7 1,633,319 - -0.9 1,633,319 - -2.0 1,633,319 - +0.3 1,633,319 - -0.7 1,633,330 + +1.3 1,633,366 + -1.5 1,633,370 - +0.3 1,633,553 + Pf1N1B4_1425 0.24 +1.6 1,633,554 - Pf1N1B4_1425 0.24 +0.3 1,633,569 + Pf1N1B4_1425 0.26 -1.7 1,633,569 + Pf1N1B4_1425 0.26 -1.9 1,633,569 + Pf1N1B4_1425 0.26 -0.3 1,633,570 - Pf1N1B4_1425 0.26 -0.9 1,633,570 - Pf1N1B4_1425 0.26 -1.1 1,633,570 - Pf1N1B4_1425 0.26 -1.0 1,633,570 - Pf1N1B4_1425 0.26 -0.7
Or see this region's nucleotide sequence