Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1366

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1364 and Pf1N1B4_1365 are separated by 106 nucleotidesPf1N1B4_1365 and Pf1N1B4_1366 are separated by 0 nucleotidesPf1N1B4_1366 and Pf1N1B4_1367 are separated by 123 nucleotides Pf1N1B4_1364 - Lipase precursor (EC 3.1.1.3), at 1,562,928 to 1,563,818 _1364 Pf1N1B4_1365 - Quaternary ammonium compound-resistance protein SugE, at 1,563,925 to 1,564,230 _1365 Pf1N1B4_1366 - FMN-dependent NADH-azoreductase, at 1,564,231 to 1,564,869 _1366 Pf1N1B4_1367 - Glycine cleavage system transcriptional activator, at 1,564,993 to 1,565,934 _1367 Position (kb) 1564 1565Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1563.412 kb on - strand, within Pf1N1B4_1364at 1563.412 kb on - strand, within Pf1N1B4_1364at 1563.459 kb on - strand, within Pf1N1B4_1364at 1563.459 kb on - strand, within Pf1N1B4_1364at 1563.459 kb on - strand, within Pf1N1B4_1364at 1563.459 kb on - strand, within Pf1N1B4_1364at 1563.459 kb on - strand, within Pf1N1B4_1364at 1563.466 kb on + strand, within Pf1N1B4_1364at 1563.466 kb on + strand, within Pf1N1B4_1364at 1563.466 kb on + strand, within Pf1N1B4_1364at 1563.466 kb on + strand, within Pf1N1B4_1364at 1563.467 kb on - strand, within Pf1N1B4_1364at 1563.730 kb on + strandat 1563.736 kb on + strandat 1563.914 kb on + strandat 1563.914 kb on + strandat 1563.915 kb on - strandat 1563.952 kb on + strandat 1564.101 kb on - strand, within Pf1N1B4_1365at 1564.101 kb on - strand, within Pf1N1B4_1365at 1564.166 kb on + strand, within Pf1N1B4_1365at 1564.167 kb on - strand, within Pf1N1B4_1365at 1564.167 kb on - strand, within Pf1N1B4_1365at 1564.167 kb on - strand, within Pf1N1B4_1365at 1564.232 kb on + strandat 1564.232 kb on + strandat 1564.232 kb on + strandat 1564.233 kb on - strandat 1564.233 kb on - strandat 1564.233 kb on - strandat 1564.233 kb on - strandat 1564.479 kb on - strand, within Pf1N1B4_1366at 1564.479 kb on - strand, within Pf1N1B4_1366at 1564.577 kb on + strand, within Pf1N1B4_1366at 1564.577 kb on + strandat 1564.578 kb on - strand, within Pf1N1B4_1366at 1564.578 kb on - strand, within Pf1N1B4_1366at 1564.578 kb on - strandat 1564.989 kb on + strandat 1564.990 kb on - strandat 1565.459 kb on - strand, within Pf1N1B4_1367at 1565.459 kb on - strand, within Pf1N1B4_1367at 1565.494 kb on + strand, within Pf1N1B4_1367at 1565.494 kb on + strand, within Pf1N1B4_1367at 1565.494 kb on + strand, within Pf1N1B4_1367at 1565.575 kb on + strand, within Pf1N1B4_1367at 1565.575 kb on + strand, within Pf1N1B4_1367at 1565.576 kb on - strand, within Pf1N1B4_1367at 1565.576 kb on - strand, within Pf1N1B4_1367at 1565.650 kb on + strand, within Pf1N1B4_1367at 1565.650 kb on + strand, within Pf1N1B4_1367at 1565.792 kb on - strand, within Pf1N1B4_1367at 1565.792 kb on - strand, within Pf1N1B4_1367

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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1,563,412 - Pf1N1B4_1364 0.54 -1.1
1,563,412 - Pf1N1B4_1364 0.54 +0.2
1,563,459 - Pf1N1B4_1364 0.60 -1.5
1,563,459 - Pf1N1B4_1364 0.60 -0.7
1,563,459 - Pf1N1B4_1364 0.60 +0.6
1,563,459 - Pf1N1B4_1364 0.60 -0.7
1,563,459 - Pf1N1B4_1364 0.60 +0.1
1,563,466 + Pf1N1B4_1364 0.60 +0.1
1,563,466 + Pf1N1B4_1364 0.60 -0.2
1,563,466 + Pf1N1B4_1364 0.60 +0.9
1,563,466 + Pf1N1B4_1364 0.60 +0.7
1,563,467 - Pf1N1B4_1364 0.60 -2.0
1,563,730 + -2.8
1,563,736 + -0.0
1,563,914 + -2.4
1,563,914 + +0.3
1,563,915 - -1.0
1,563,952 + -0.3
1,564,101 - Pf1N1B4_1365 0.58 +1.3
1,564,101 - Pf1N1B4_1365 0.58 -0.6
1,564,166 + Pf1N1B4_1365 0.79 +1.4
1,564,167 - Pf1N1B4_1365 0.79 +1.9
1,564,167 - Pf1N1B4_1365 0.79 +0.5
1,564,167 - Pf1N1B4_1365 0.79 -0.1
1,564,232 + -1.9
1,564,232 + -0.6
1,564,232 + -1.7
1,564,233 - +1.0
1,564,233 - +0.4
1,564,233 - -1.3
1,564,233 - +0.7
1,564,479 - Pf1N1B4_1366 0.39 -1.7
1,564,479 - Pf1N1B4_1366 0.39 -0.1
1,564,577 + Pf1N1B4_1366 0.54 -0.5
1,564,577 + +0.3
1,564,578 - Pf1N1B4_1366 0.54 -1.1
1,564,578 - Pf1N1B4_1366 0.54 -0.4
1,564,578 - +1.3
1,564,989 + -0.1
1,564,990 - -0.5
1,565,459 - Pf1N1B4_1367 0.49 +0.5
1,565,459 - Pf1N1B4_1367 0.49 +0.1
1,565,494 + Pf1N1B4_1367 0.53 -1.0
1,565,494 + Pf1N1B4_1367 0.53 -0.7
1,565,494 + Pf1N1B4_1367 0.53 +0.9
1,565,575 + Pf1N1B4_1367 0.62 -1.0
1,565,575 + Pf1N1B4_1367 0.62 -0.5
1,565,576 - Pf1N1B4_1367 0.62 -0.3
1,565,576 - Pf1N1B4_1367 0.62 -0.0
1,565,650 + Pf1N1B4_1367 0.70 +1.0
1,565,650 + Pf1N1B4_1367 0.70 -2.0
1,565,792 - Pf1N1B4_1367 0.85 -0.7
1,565,792 - Pf1N1B4_1367 0.85 +0.1

Or see this region's nucleotide sequence