Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1332 and Pf1N1B4_1333 overlap by 4 nucleotides Pf1N1B4_1333 and Pf1N1B4_1334 are separated by 272 nucleotides Pf1N1B4_1334 and Pf1N1B4_1335 are separated by 48 nucleotides
Pf1N1B4_1332 - Cobalt-precorrin-6 synthase, anaerobic, at 1,527,328 to 1,528,425
_1332
Pf1N1B4_1333 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 1,528,422 to 1,529,156
_1333
Pf1N1B4_1334 - FIG00955840: hypothetical protein, at 1,529,429 to 1,529,827
_1334
Pf1N1B4_1335 - Copper metallochaperone, bacterial analog of Cox17 protein, at 1,529,876 to 1,530,355
_1335
Position (kb)
1528
1529
1530 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1527.757 kb on + strand, within Pf1N1B4_1332 at 1527.901 kb on + strand, within Pf1N1B4_1332 at 1527.902 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.252 kb on - strand, within Pf1N1B4_1332 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand, within Pf1N1B4_1333 at 1528.497 kb on + strand at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1528.498 kb on - strand, within Pf1N1B4_1333 at 1529.139 kb on + strand at 1529.139 kb on + strand at 1529.139 kb on + strand at 1529.140 kb on - strand at 1529.140 kb on - strand at 1529.140 kb on - strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.154 kb on + strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.155 kb on - strand at 1529.158 kb on + strand at 1529.158 kb on + strand at 1529.159 kb on - strand at 1529.277 kb on + strand at 1529.278 kb on - strand at 1529.363 kb on + strand at 1529.363 kb on + strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.364 kb on - strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.385 kb on + strand at 1529.386 kb on - strand at 1529.386 kb on - strand at 1529.389 kb on + strand at 1529.390 kb on - strand at 1529.390 kb on - strand at 1529.390 kb on - strand at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.717 kb on + strand, within Pf1N1B4_1334 at 1529.718 kb on - strand, within Pf1N1B4_1334 at 1529.721 kb on - strand, within Pf1N1B4_1334 at 1529.774 kb on - strand at 1529.904 kb on + strand at 1529.905 kb on - strand at 1529.905 kb on - strand at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.942 kb on + strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1529.943 kb on - strand, within Pf1N1B4_1335 at 1530.017 kb on + strand, within Pf1N1B4_1335 at 1530.018 kb on - strand, within Pf1N1B4_1335
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,527,757 + Pf1N1B4_1332 0.39 -0.7 1,527,901 + Pf1N1B4_1332 0.52 -0.9 1,527,902 - Pf1N1B4_1332 0.52 -0.1 1,528,007 - Pf1N1B4_1332 0.62 -0.5 1,528,007 - Pf1N1B4_1332 0.62 -0.1 1,528,252 - Pf1N1B4_1332 0.84 -1.1 1,528,497 + Pf1N1B4_1333 0.10 +0.9 1,528,497 + Pf1N1B4_1333 0.10 +0.4 1,528,497 + Pf1N1B4_1333 0.10 +0.8 1,528,497 + Pf1N1B4_1333 0.10 -1.9 1,528,497 + Pf1N1B4_1333 0.10 +0.9 1,528,497 + Pf1N1B4_1333 0.10 +0.9 1,528,497 + Pf1N1B4_1333 0.10 -1.3 1,528,497 + Pf1N1B4_1333 0.10 -1.3 1,528,497 + Pf1N1B4_1333 0.10 -0.3 1,528,497 + Pf1N1B4_1333 0.10 +1.3 1,528,497 + Pf1N1B4_1333 0.10 +2.9 1,528,497 + Pf1N1B4_1333 0.10 +0.6 1,528,497 + Pf1N1B4_1333 0.10 -1.9 1,528,497 + Pf1N1B4_1333 0.10 +1.8 1,528,497 + Pf1N1B4_1333 0.10 +0.7 1,528,497 + -0.7 1,528,498 - Pf1N1B4_1333 0.10 +1.1 1,528,498 - Pf1N1B4_1333 0.10 -0.3 1,528,498 - Pf1N1B4_1333 0.10 -0.7 1,528,498 - Pf1N1B4_1333 0.10 +0.4 1,528,498 - Pf1N1B4_1333 0.10 +0.6 1,528,498 - Pf1N1B4_1333 0.10 -0.3 1,529,139 + -2.2 1,529,139 + +0.1 1,529,139 + -0.2 1,529,140 - -0.7 1,529,140 - +0.9 1,529,140 - -0.4 1,529,154 + -0.1 1,529,154 + +0.0 1,529,154 + -0.6 1,529,154 + -0.3 1,529,154 + -2.1 1,529,154 + +1.3 1,529,155 - +0.5 1,529,155 - +0.5 1,529,155 - -0.3 1,529,155 - -1.1 1,529,158 + -1.2 1,529,158 + -0.3 1,529,159 - -1.7 1,529,277 + +0.1 1,529,278 - -1.3 1,529,363 + +0.9 1,529,363 + +0.6 1,529,364 - -0.7 1,529,364 - -2.2 1,529,364 - -1.1 1,529,364 - -1.1 1,529,385 + -1.1 1,529,385 + +0.0 1,529,385 + +0.9 1,529,385 + -0.7 1,529,385 + +1.6 1,529,385 + -0.7 1,529,386 - +0.4 1,529,386 - +0.5 1,529,389 + -2.1 1,529,390 - -0.2 1,529,390 - -0.8 1,529,390 - -2.1 1,529,717 + Pf1N1B4_1334 0.72 +0.5 1,529,717 + Pf1N1B4_1334 0.72 +0.9 1,529,717 + Pf1N1B4_1334 0.72 +1.3 1,529,718 - Pf1N1B4_1334 0.72 -0.1 1,529,721 - Pf1N1B4_1334 0.73 +0.4 1,529,774 - +1.3 1,529,904 + -1.9 1,529,905 - -0.5 1,529,905 - -0.9 1,529,942 + Pf1N1B4_1335 0.14 +1.9 1,529,942 + Pf1N1B4_1335 0.14 +1.0 1,529,942 + Pf1N1B4_1335 0.14 -0.1 1,529,942 + Pf1N1B4_1335 0.14 -1.4 1,529,942 + Pf1N1B4_1335 0.14 -0.7 1,529,943 - Pf1N1B4_1335 0.14 -0.8 1,529,943 - Pf1N1B4_1335 0.14 -0.5 1,529,943 - Pf1N1B4_1335 0.14 -0.7 1,530,017 + Pf1N1B4_1335 0.29 -0.7 1,530,018 - Pf1N1B4_1335 0.30 -1.3
Or see this region's nucleotide sequence