Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotides Pf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides Pf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotides Pf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides
Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882
_122
Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332
_123
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839
_125
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.675 kb on + strand, within Pf1N1B4_122 at 134.676 kb on - strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.773 kb on - strand at 134.894 kb on - strand at 135.011 kb on - strand, within Pf1N1B4_123 at 135.011 kb on - strand, within Pf1N1B4_123 at 135.311 kb on - strand at 135.389 kb on - strand at 135.389 kb on - strand at 135.394 kb on + strand at 135.403 kb on - strand at 135.403 kb on - strand at 135.425 kb on + strand at 135.526 kb on + strand at 135.735 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 137.040 kb on + strand at 137.041 kb on - strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.044 kb on - strand at 137.060 kb on + strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.128 kb on - strand at 137.189 kb on + strand at 137.189 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.214 kb on + strand at 137.215 kb on - strand at 137.238 kb on + strand at 137.239 kb on - strand at 137.239 kb on - strand at 137.695 kb on - strand, within Pf1N1B4_125 at 137.834 kb on - strand at 137.834 kb on - strand at 137.924 kb on + strand at 137.955 kb on - strand at 137.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 134,675 + Pf1N1B4_122 0.87 -0.1 134,676 - +2.1 134,772 + -0.0 134,772 + +0.7 134,772 + -1.9 134,773 - +1.3 134,894 - -0.9 135,011 - Pf1N1B4_123 0.27 -0.9 135,011 - Pf1N1B4_123 0.27 +0.4 135,311 - -0.3 135,389 - +1.1 135,389 - -0.0 135,394 + +0.3 135,403 - +0.5 135,403 - -0.6 135,425 + -2.3 135,526 + -0.9 135,735 + Pf1N1B4_124 0.11 -3.1 136,647 + Pf1N1B4_124 0.74 -0.4 136,647 + Pf1N1B4_124 0.74 +0.1 136,648 - Pf1N1B4_124 0.74 -0.0 136,648 - Pf1N1B4_124 0.74 -2.6 137,040 + -1.9 137,041 - -0.6 137,043 + +0.5 137,044 - -2.7 137,044 - +0.1 137,060 + +1.1 137,061 - -0.3 137,061 - +1.1 137,061 - +0.4 137,128 - +0.7 137,189 + -1.6 137,189 + -0.4 137,197 + +0.7 137,197 + +0.3 137,197 + -0.9 137,197 + +0.3 137,197 + -1.0 137,198 - -0.9 137,198 - -0.3 137,198 - +0.7 137,198 - +0.1 137,198 - -1.3 137,214 + -1.6 137,215 - -0.6 137,238 + -1.3 137,239 - -0.3 137,239 - +0.4 137,695 - Pf1N1B4_125 0.77 -0.6 137,834 - -0.9 137,834 - +0.7 137,924 + +1.1 137,955 - -0.0 137,955 - -0.0
Or see this region's nucleotide sequence