Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1103

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1102 and Pf1N1B4_1103 are separated by 148 nucleotidesPf1N1B4_1103 and Pf1N1B4_1104 are separated by 50 nucleotides Pf1N1B4_1102 - Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7), at 1,235,177 to 1,236,622 _1102 Pf1N1B4_1103 - Rare lipoprotein A, at 1,236,771 to 1,237,145 _1103 Pf1N1B4_1104 - K+-dependent Na+/Ca+ exchanger related-protein, at 1,237,196 to 1,238,257 _1104 Position (kb) 1236 1237 1238Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1236.764 kb on - strandat 1236.770 kb on + strandat 1236.770 kb on + strandat 1236.770 kb on + strandat 1236.771 kb on - strandat 1236.771 kb on - strandat 1236.771 kb on - strandat 1236.869 kb on - strand, within Pf1N1B4_1103at 1236.869 kb on - strand, within Pf1N1B4_1103at 1236.869 kb on - strand, within Pf1N1B4_1103at 1236.869 kb on - strand, within Pf1N1B4_1103at 1236.876 kb on + strand, within Pf1N1B4_1103at 1236.877 kb on - strand, within Pf1N1B4_1103at 1236.877 kb on - strand, within Pf1N1B4_1103at 1236.877 kb on - strand, within Pf1N1B4_1103at 1236.880 kb on - strand, within Pf1N1B4_1103at 1236.986 kb on + strand, within Pf1N1B4_1103at 1237.042 kb on - strand, within Pf1N1B4_1103at 1237.042 kb on - strand, within Pf1N1B4_1103at 1237.147 kb on - strandat 1237.162 kb on + strandat 1237.162 kb on + strandat 1237.182 kb on + strandat 1237.182 kb on + strandat 1237.182 kb on + strandat 1237.224 kb on + strandat 1237.225 kb on - strandat 1237.574 kb on - strand, within Pf1N1B4_1104at 1237.574 kb on - strand, within Pf1N1B4_1104at 1237.598 kb on + strand, within Pf1N1B4_1104at 1237.599 kb on - strand, within Pf1N1B4_1104at 1237.599 kb on - strand, within Pf1N1B4_1104at 1237.612 kb on - strand, within Pf1N1B4_1104at 1237.893 kb on + strand, within Pf1N1B4_1104at 1237.893 kb on + strand, within Pf1N1B4_1104at 1237.893 kb on + strand, within Pf1N1B4_1104at 1237.893 kb on + strand, within Pf1N1B4_1104at 1237.894 kb on - strand, within Pf1N1B4_1104at 1237.930 kb on - strand, within Pf1N1B4_1104at 1237.930 kb on - strand, within Pf1N1B4_1104at 1238.040 kb on - strand, within Pf1N1B4_1104at 1238.040 kb on - strand, within Pf1N1B4_1104at 1238.090 kb on + strand, within Pf1N1B4_1104at 1238.090 kb on + strand, within Pf1N1B4_1104at 1238.099 kb on + strand, within Pf1N1B4_1104at 1238.100 kb on - strandat 1238.100 kb on - strand, within Pf1N1B4_1104

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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1,236,764 - +1.2
1,236,770 + +0.2
1,236,770 + +1.3
1,236,770 + -1.6
1,236,771 - -1.3
1,236,771 - +0.7
1,236,771 - -0.6
1,236,869 - Pf1N1B4_1103 0.26 +0.7
1,236,869 - Pf1N1B4_1103 0.26 -2.6
1,236,869 - Pf1N1B4_1103 0.26 -1.1
1,236,869 - Pf1N1B4_1103 0.26 +0.2
1,236,876 + Pf1N1B4_1103 0.28 -1.3
1,236,877 - Pf1N1B4_1103 0.28 -2.1
1,236,877 - Pf1N1B4_1103 0.28 -1.5
1,236,877 - Pf1N1B4_1103 0.28 +1.2
1,236,880 - Pf1N1B4_1103 0.29 -1.6
1,236,986 + Pf1N1B4_1103 0.57 +0.4
1,237,042 - Pf1N1B4_1103 0.72 -1.7
1,237,042 - Pf1N1B4_1103 0.72 -0.0
1,237,147 - +1.5
1,237,162 + -1.3
1,237,162 + +0.5
1,237,182 + -1.8
1,237,182 + -0.6
1,237,182 + -1.6
1,237,224 + +2.8
1,237,225 - +0.0
1,237,574 - Pf1N1B4_1104 0.36 +1.9
1,237,574 - Pf1N1B4_1104 0.36 -1.8
1,237,598 + Pf1N1B4_1104 0.38 +0.3
1,237,599 - Pf1N1B4_1104 0.38 -1.3
1,237,599 - Pf1N1B4_1104 0.38 -0.4
1,237,612 - Pf1N1B4_1104 0.39 +0.2
1,237,893 + Pf1N1B4_1104 0.66 -2.1
1,237,893 + Pf1N1B4_1104 0.66 +0.9
1,237,893 + Pf1N1B4_1104 0.66 -0.3
1,237,893 + Pf1N1B4_1104 0.66 +0.8
1,237,894 - Pf1N1B4_1104 0.66 +1.0
1,237,930 - Pf1N1B4_1104 0.69 -2.0
1,237,930 - Pf1N1B4_1104 0.69 +0.2
1,238,040 - Pf1N1B4_1104 0.79 -0.4
1,238,040 - Pf1N1B4_1104 0.79 +0.8
1,238,090 + Pf1N1B4_1104 0.84 +0.1
1,238,090 + Pf1N1B4_1104 0.84 -0.3
1,238,099 + Pf1N1B4_1104 0.85 +0.4
1,238,100 - +1.2
1,238,100 - Pf1N1B4_1104 0.85 -0.1

Or see this region's nucleotide sequence