Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1102 and Pf1N1B4_1103 are separated by 148 nucleotides Pf1N1B4_1103 and Pf1N1B4_1104 are separated by 50 nucleotides
Pf1N1B4_1102 - Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7), at 1,235,177 to 1,236,622
_1102
Pf1N1B4_1103 - Rare lipoprotein A, at 1,236,771 to 1,237,145
_1103
Pf1N1B4_1104 - K+-dependent Na+/Ca+ exchanger related-protein, at 1,237,196 to 1,238,257
_1104
Position (kb)
1236
1237
1238 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1236.764 kb on - strand at 1236.770 kb on + strand at 1236.770 kb on + strand at 1236.770 kb on + strand at 1236.771 kb on - strand at 1236.771 kb on - strand at 1236.771 kb on - strand at 1236.869 kb on - strand, within Pf1N1B4_1103 at 1236.869 kb on - strand, within Pf1N1B4_1103 at 1236.869 kb on - strand, within Pf1N1B4_1103 at 1236.869 kb on - strand, within Pf1N1B4_1103 at 1236.876 kb on + strand, within Pf1N1B4_1103 at 1236.877 kb on - strand, within Pf1N1B4_1103 at 1236.877 kb on - strand, within Pf1N1B4_1103 at 1236.877 kb on - strand, within Pf1N1B4_1103 at 1236.880 kb on - strand, within Pf1N1B4_1103 at 1236.986 kb on + strand, within Pf1N1B4_1103 at 1237.042 kb on - strand, within Pf1N1B4_1103 at 1237.042 kb on - strand, within Pf1N1B4_1103 at 1237.147 kb on - strand at 1237.162 kb on + strand at 1237.162 kb on + strand at 1237.182 kb on + strand at 1237.182 kb on + strand at 1237.182 kb on + strand at 1237.224 kb on + strand at 1237.225 kb on - strand at 1237.574 kb on - strand, within Pf1N1B4_1104 at 1237.574 kb on - strand, within Pf1N1B4_1104 at 1237.598 kb on + strand, within Pf1N1B4_1104 at 1237.599 kb on - strand, within Pf1N1B4_1104 at 1237.599 kb on - strand, within Pf1N1B4_1104 at 1237.612 kb on - strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.894 kb on - strand, within Pf1N1B4_1104 at 1237.930 kb on - strand, within Pf1N1B4_1104 at 1237.930 kb on - strand, within Pf1N1B4_1104 at 1238.040 kb on - strand, within Pf1N1B4_1104 at 1238.040 kb on - strand, within Pf1N1B4_1104 at 1238.090 kb on + strand, within Pf1N1B4_1104 at 1238.090 kb on + strand, within Pf1N1B4_1104 at 1238.099 kb on + strand, within Pf1N1B4_1104 at 1238.100 kb on - strand at 1238.100 kb on - strand, within Pf1N1B4_1104
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,236,764 - +1.2 1,236,770 + +0.2 1,236,770 + +1.3 1,236,770 + -1.6 1,236,771 - -1.3 1,236,771 - +0.7 1,236,771 - -0.6 1,236,869 - Pf1N1B4_1103 0.26 +0.7 1,236,869 - Pf1N1B4_1103 0.26 -2.6 1,236,869 - Pf1N1B4_1103 0.26 -1.1 1,236,869 - Pf1N1B4_1103 0.26 +0.2 1,236,876 + Pf1N1B4_1103 0.28 -1.3 1,236,877 - Pf1N1B4_1103 0.28 -2.1 1,236,877 - Pf1N1B4_1103 0.28 -1.5 1,236,877 - Pf1N1B4_1103 0.28 +1.2 1,236,880 - Pf1N1B4_1103 0.29 -1.6 1,236,986 + Pf1N1B4_1103 0.57 +0.4 1,237,042 - Pf1N1B4_1103 0.72 -1.7 1,237,042 - Pf1N1B4_1103 0.72 -0.0 1,237,147 - +1.5 1,237,162 + -1.3 1,237,162 + +0.5 1,237,182 + -1.8 1,237,182 + -0.6 1,237,182 + -1.6 1,237,224 + +2.8 1,237,225 - +0.0 1,237,574 - Pf1N1B4_1104 0.36 +1.9 1,237,574 - Pf1N1B4_1104 0.36 -1.8 1,237,598 + Pf1N1B4_1104 0.38 +0.3 1,237,599 - Pf1N1B4_1104 0.38 -1.3 1,237,599 - Pf1N1B4_1104 0.38 -0.4 1,237,612 - Pf1N1B4_1104 0.39 +0.2 1,237,893 + Pf1N1B4_1104 0.66 -2.1 1,237,893 + Pf1N1B4_1104 0.66 +0.9 1,237,893 + Pf1N1B4_1104 0.66 -0.3 1,237,893 + Pf1N1B4_1104 0.66 +0.8 1,237,894 - Pf1N1B4_1104 0.66 +1.0 1,237,930 - Pf1N1B4_1104 0.69 -2.0 1,237,930 - Pf1N1B4_1104 0.69 +0.2 1,238,040 - Pf1N1B4_1104 0.79 -0.4 1,238,040 - Pf1N1B4_1104 0.79 +0.8 1,238,090 + Pf1N1B4_1104 0.84 +0.1 1,238,090 + Pf1N1B4_1104 0.84 -0.3 1,238,099 + Pf1N1B4_1104 0.85 +0.4 1,238,100 - +1.2 1,238,100 - Pf1N1B4_1104 0.85 -0.1
Or see this region's nucleotide sequence