Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_102 and Pf1N1B4_103 are separated by 108 nucleotides Pf1N1B4_103 and Pf1N1B4_104 are separated by 493 nucleotides
Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446
_102
Pf1N1B4_103 - Histidine permease YuiF, at 108,555 to 109,871
_103
Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678
_104
Position (kb) 108
109
110 Strain fitness (log2 ratio) -2
-1
0
1 at 107.716 kb on - strand, within Pf1N1B4_102 at 107.921 kb on - strand, within Pf1N1B4_102 at 107.974 kb on + strand, within Pf1N1B4_102 at 108.091 kb on + strand, within Pf1N1B4_102 at 108.196 kb on + strand, within Pf1N1B4_102 at 108.408 kb on + strand at 108.409 kb on - strand at 108.409 kb on - strand at 108.409 kb on - strand at 108.495 kb on + strand at 108.495 kb on + strand at 108.556 kb on + strand at 108.557 kb on - strand at 108.557 kb on - strand at 108.605 kb on - strand at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand, within Pf1N1B4_103 at 109.175 kb on - strand at 109.219 kb on + strand, within Pf1N1B4_103 at 109.324 kb on - strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.399 kb on + strand, within Pf1N1B4_103 at 109.400 kb on - strand, within Pf1N1B4_103 at 109.471 kb on + strand, within Pf1N1B4_103 at 109.471 kb on + strand at 109.471 kb on + strand, within Pf1N1B4_103 at 109.472 kb on - strand at 109.472 kb on - strand, within Pf1N1B4_103 at 109.472 kb on - strand, within Pf1N1B4_103 at 109.473 kb on + strand, within Pf1N1B4_103 at 109.521 kb on + strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.522 kb on - strand, within Pf1N1B4_103 at 109.834 kb on - strand at 109.861 kb on - strand at 109.942 kb on - strand at 109.942 kb on - strand at 109.976 kb on - strand at 109.976 kb on - strand at 109.978 kb on + strand at 110.134 kb on + strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.135 kb on - strand at 110.238 kb on - strand at 110.443 kb on - strand at 110.715 kb on - strand, within Pf1N1B4_104
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 107,716 - Pf1N1B4_102 0.58 +1.6 107,921 - Pf1N1B4_102 0.70 -1.6 107,974 + Pf1N1B4_102 0.73 -0.9 108,091 + Pf1N1B4_102 0.80 +0.5 108,196 + Pf1N1B4_102 0.86 +1.1 108,408 + -0.5 108,409 - +0.2 108,409 - -2.1 108,409 - +1.0 108,495 + +0.4 108,495 + +1.6 108,556 + +1.2 108,557 - +0.2 108,557 - -0.0 108,605 - -0.6 109,175 - Pf1N1B4_103 0.47 -1.6 109,175 - Pf1N1B4_103 0.47 -0.9 109,175 - Pf1N1B4_103 0.47 -1.3 109,175 - Pf1N1B4_103 0.47 -0.6 109,175 - Pf1N1B4_103 0.47 -1.3 109,175 - Pf1N1B4_103 0.47 -0.9 109,175 - Pf1N1B4_103 0.47 -0.4 109,175 - Pf1N1B4_103 0.47 -1.3 109,175 - Pf1N1B4_103 0.47 -0.3 109,175 - Pf1N1B4_103 0.47 -0.9 109,175 - -0.6 109,219 + Pf1N1B4_103 0.50 -1.6 109,324 - Pf1N1B4_103 0.58 -2.3 109,399 + Pf1N1B4_103 0.64 -1.1 109,399 + Pf1N1B4_103 0.64 -0.3 109,399 + Pf1N1B4_103 0.64 -0.6 109,400 - Pf1N1B4_103 0.64 -1.6 109,471 + Pf1N1B4_103 0.70 +0.7 109,471 + -0.9 109,471 + Pf1N1B4_103 0.70 +0.7 109,472 - +0.7 109,472 - Pf1N1B4_103 0.70 -1.6 109,472 - Pf1N1B4_103 0.70 -0.1 109,473 + Pf1N1B4_103 0.70 -1.3 109,521 + Pf1N1B4_103 0.73 -1.4 109,522 - Pf1N1B4_103 0.73 -1.3 109,522 - Pf1N1B4_103 0.73 +0.3 109,834 - -0.6 109,861 - -0.1 109,942 - -0.1 109,942 - -0.3 109,976 - +0.1 109,976 - -0.5 109,978 + +0.3 110,134 + -1.1 110,135 - -0.6 110,135 - +0.1 110,135 - -0.9 110,238 - -0.9 110,443 - -0.7 110,715 - Pf1N1B4_104 0.27 -1.1
Or see this region's nucleotide sequence