Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05245 and MMP_RS05250 are separated by 117 nucleotides MMP_RS05250 and MMP_RS05255 are separated by 205 nucleotides MMP_RS05255 and MMP_RS05260 are separated by 41 nucleotides
MMP_RS05245: MMP_RS05245 - archaeosine biosynthesis radical SAM protein RaSEA, at 1,001,423 to 1,002,490
_RS05245
MMP_RS05250: MMP_RS05250 - NFYB/HAP3 family transcription factor subunit, at 1,002,608 to 1,002,907
_RS05250
MMP_RS05255: MMP_RS05255 - CBS domain-containing protein, at 1,003,113 to 1,004,354
_RS05255
MMP_RS05260: MMP_RS05260 - aspartate kinase, at 1,004,396 to 1,005,802
_RS05260
Position (kb)
1003
1004
1005 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1002.113 kb on + strand, within MMP_RS05245 at 1002.113 kb on + strand, within MMP_RS05245 at 1002.115 kb on + strand, within MMP_RS05245 at 1002.115 kb on + strand, within MMP_RS05245 at 1002.172 kb on + strand, within MMP_RS05245 at 1002.316 kb on - strand, within MMP_RS05245 at 1002.357 kb on - strand, within MMP_RS05245 at 1002.357 kb on - strand, within MMP_RS05245 at 1002.434 kb on - strand at 1002.435 kb on + strand at 1002.435 kb on + strand at 1002.503 kb on + strand at 1002.548 kb on - strand at 1002.550 kb on - strand at 1002.551 kb on + strand at 1002.576 kb on + strand at 1002.607 kb on - strand at 1002.612 kb on - strand at 1002.638 kb on + strand, within MMP_RS05250 at 1002.663 kb on - strand, within MMP_RS05250 at 1002.859 kb on - strand, within MMP_RS05250 at 1002.860 kb on + strand, within MMP_RS05250 at 1002.903 kb on + strand at 1002.903 kb on + strand at 1002.948 kb on - strand at 1002.949 kb on + strand at 1002.949 kb on + strand at 1002.954 kb on + strand at 1002.968 kb on + strand at 1002.995 kb on - strand at 1002.996 kb on + strand at 1003.126 kb on - strand at 1003.136 kb on + strand at 1003.197 kb on + strand at 1003.226 kb on + strand at 1003.246 kb on - strand, within MMP_RS05255 at 1003.246 kb on - strand, within MMP_RS05255 at 1003.246 kb on - strand, within MMP_RS05255 at 1003.246 kb on - strand, within MMP_RS05255 at 1003.246 kb on - strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.247 kb on + strand, within MMP_RS05255 at 1003.248 kb on - strand, within MMP_RS05255 at 1003.249 kb on + strand, within MMP_RS05255 at 1003.249 kb on + strand, within MMP_RS05255 at 1003.249 kb on + strand, within MMP_RS05255 at 1003.249 kb on + strand, within MMP_RS05255 at 1003.249 kb on + strand, within MMP_RS05255 at 1003.252 kb on - strand, within MMP_RS05255 at 1003.253 kb on + strand, within MMP_RS05255 at 1003.253 kb on + strand, within MMP_RS05255 at 1003.440 kb on - strand, within MMP_RS05255 at 1003.561 kb on - strand, within MMP_RS05255 at 1003.574 kb on - strand, within MMP_RS05255 at 1003.589 kb on + strand, within MMP_RS05255 at 1003.643 kb on + strand, within MMP_RS05255 at 1003.643 kb on + strand, within MMP_RS05255 at 1003.646 kb on - strand, within MMP_RS05255 at 1003.692 kb on + strand, within MMP_RS05255 at 1003.739 kb on + strand, within MMP_RS05255 at 1003.752 kb on + strand, within MMP_RS05255 at 1003.752 kb on + strand, within MMP_RS05255 at 1004.036 kb on - strand, within MMP_RS05255 at 1004.070 kb on - strand, within MMP_RS05255 at 1004.085 kb on - strand, within MMP_RS05255 at 1004.110 kb on + strand, within MMP_RS05255 at 1004.179 kb on - strand, within MMP_RS05255 at 1004.353 kb on + strand at 1004.518 kb on - strand at 1004.610 kb on + strand at 1004.636 kb on - strand at 1004.666 kb on - strand at 1004.707 kb on + strand at 1004.756 kb on - strand at 1004.834 kb on - strand at 1004.834 kb on - strand at 1004.834 kb on - strand at 1004.877 kb on + strand at 1004.917 kb on - strand at 1005.018 kb on - strand at 1005.043 kb on + strand at 1005.168 kb on - strand at 1005.169 kb on + strand at 1005.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 1,002,113 + MMP_RS05245 0.65 -1.6 1,002,113 + MMP_RS05245 0.65 -1.4 1,002,115 + MMP_RS05245 0.65 -1.2 1,002,115 + MMP_RS05245 0.65 -0.9 1,002,172 + MMP_RS05245 0.70 -1.1 1,002,316 - MMP_RS05245 0.84 -1.4 1,002,357 - MMP_RS05245 0.87 -0.9 1,002,357 - MMP_RS05245 0.87 -1.7 1,002,434 - -0.4 1,002,435 + -1.4 1,002,435 + -1.6 1,002,503 + +0.2 1,002,548 - -0.5 1,002,550 - +0.2 1,002,551 + -1.4 1,002,576 + -1.6 1,002,607 - -1.6 1,002,612 - -4.6 1,002,638 + MMP_RS05250 0.10 -2.5 1,002,663 - MMP_RS05250 0.18 -3.4 1,002,859 - MMP_RS05250 0.84 -2.8 1,002,860 + MMP_RS05250 0.84 -2.7 1,002,903 + +0.4 1,002,903 + +0.3 1,002,948 - -0.2 1,002,949 + -0.1 1,002,949 + -0.7 1,002,954 + -0.2 1,002,968 + +0.1 1,002,995 - -0.2 1,002,996 + +0.1 1,003,126 - -1.8 1,003,136 + -3.3 1,003,197 + -2.6 1,003,226 + -1.4 1,003,246 - MMP_RS05255 0.11 -2.1 1,003,246 - MMP_RS05255 0.11 -2.8 1,003,246 - MMP_RS05255 0.11 -1.7 1,003,246 - MMP_RS05255 0.11 -1.4 1,003,246 - MMP_RS05255 0.11 -2.1 1,003,247 + MMP_RS05255 0.11 -2.7 1,003,247 + MMP_RS05255 0.11 -2.7 1,003,247 + MMP_RS05255 0.11 -2.1 1,003,247 + MMP_RS05255 0.11 -2.5 1,003,247 + MMP_RS05255 0.11 -2.0 1,003,247 + MMP_RS05255 0.11 -3.4 1,003,248 - MMP_RS05255 0.11 -2.2 1,003,249 + MMP_RS05255 0.11 -2.2 1,003,249 + MMP_RS05255 0.11 -2.3 1,003,249 + MMP_RS05255 0.11 -2.8 1,003,249 + MMP_RS05255 0.11 -2.8 1,003,249 + MMP_RS05255 0.11 -1.7 1,003,252 - MMP_RS05255 0.11 -1.5 1,003,253 + MMP_RS05255 0.11 -2.5 1,003,253 + MMP_RS05255 0.11 -1.9 1,003,440 - MMP_RS05255 0.26 -2.5 1,003,561 - MMP_RS05255 0.36 -2.3 1,003,574 - MMP_RS05255 0.37 -2.5 1,003,589 + MMP_RS05255 0.38 -3.5 1,003,643 + MMP_RS05255 0.43 -1.8 1,003,643 + MMP_RS05255 0.43 -3.2 1,003,646 - MMP_RS05255 0.43 -2.6 1,003,692 + MMP_RS05255 0.47 -2.4 1,003,739 + MMP_RS05255 0.50 -2.3 1,003,752 + MMP_RS05255 0.51 -2.4 1,003,752 + MMP_RS05255 0.51 -2.1 1,004,036 - MMP_RS05255 0.74 -2.0 1,004,070 - MMP_RS05255 0.77 -1.9 1,004,085 - MMP_RS05255 0.78 -2.4 1,004,110 + MMP_RS05255 0.80 -2.4 1,004,179 - MMP_RS05255 0.86 -1.2 1,004,353 + -0.3 1,004,518 - -2.3 1,004,610 + -4.1 1,004,636 - -2.1 1,004,666 - -3.6 1,004,707 + -3.9 1,004,756 - -3.2 1,004,834 - -4.2 1,004,834 - -4.0 1,004,834 - -1.1 1,004,877 + -3.2 1,004,917 - -4.0 1,005,018 - -1.4 1,005,043 + -2.8 1,005,168 - -3.2 1,005,169 + -4.6 1,005,261 + -3.0
Or see this region's nucleotide sequence