Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02075 and MMP_RS02080 are separated by 2 nucleotides MMP_RS02080 and MMP_RS02085 are separated by 86 nucleotides MMP_RS02085 and MMP_RS02090 are separated by 65 nucleotides
MMP_RS02075: MMP_RS02075 - NAD(P)/FAD-dependent oxidoreductase, at 386,328 to 387,500
_RS02075
MMP_RS02080: MMP_RS02080 - 4Fe-4S binding protein, at 387,503 to 387,667
_RS02080
MMP_RS02085: MMP_RS02085 - inosine/xanthosine triphosphatase, at 387,754 to 388,413
_RS02085
MMP_RS02090: MMP_RS02090 - alanine--glyoxylate aminotransferase family protein, at 388,479 to 389,627
_RS02090
Position (kb) 387
388
389 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 386.779 kb on + strand at 387.073 kb on - strand at 387.273 kb on - strand at 387.292 kb on - strand at 387.396 kb on - strand at 387.430 kb on - strand at 387.607 kb on - strand at 387.654 kb on - strand at 387.679 kb on - strand at 387.691 kb on - strand at 387.692 kb on + strand at 387.715 kb on + strand at 387.743 kb on + strand at 387.743 kb on + strand at 387.755 kb on - strand at 387.816 kb on - strand at 387.820 kb on - strand, within MMP_RS02085 at 387.821 kb on + strand, within MMP_RS02085 at 387.821 kb on + strand, within MMP_RS02085 at 387.832 kb on - strand, within MMP_RS02085 at 387.832 kb on - strand, within MMP_RS02085 at 387.835 kb on - strand, within MMP_RS02085 at 387.838 kb on + strand, within MMP_RS02085 at 387.838 kb on + strand, within MMP_RS02085 at 387.952 kb on + strand, within MMP_RS02085 at 388.090 kb on - strand, within MMP_RS02085 at 388.121 kb on - strand, within MMP_RS02085 at 388.364 kb on - strand at 388.365 kb on + strand at 388.380 kb on - strand at 388.414 kb on - strand at 388.414 kb on - strand at 388.438 kb on - strand at 388.438 kb on - strand at 388.439 kb on + strand at 388.473 kb on - strand at 388.480 kb on - strand at 388.533 kb on - strand at 388.629 kb on - strand at 388.657 kb on + strand at 389.127 kb on - strand at 389.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 386,779 + -2.9 387,073 - -3.6 387,273 - -2.4 387,292 - -3.2 387,396 - -3.7 387,430 - -3.8 387,607 - -2.9 387,654 - -3.5 387,679 - -2.7 387,691 - -3.8 387,692 + -3.4 387,715 + -5.3 387,743 + -6.0 387,743 + -5.6 387,755 - -5.4 387,816 - -2.0 387,820 - MMP_RS02085 0.10 -1.7 387,821 + MMP_RS02085 0.10 -1.0 387,821 + MMP_RS02085 0.10 -1.1 387,832 - MMP_RS02085 0.12 -1.7 387,832 - MMP_RS02085 0.12 -1.5 387,835 - MMP_RS02085 0.12 -0.8 387,838 + MMP_RS02085 0.13 -1.7 387,838 + MMP_RS02085 0.13 -1.7 387,952 + MMP_RS02085 0.30 -1.2 388,090 - MMP_RS02085 0.51 -0.3 388,121 - MMP_RS02085 0.56 -0.2 388,364 - +0.2 388,365 + -1.1 388,380 - -1.3 388,414 - -0.4 388,414 - -1.2 388,438 - -0.7 388,438 - -0.7 388,439 + +0.7 388,473 - -0.6 388,480 - -0.6 388,533 - -3.7 388,629 - -2.4 388,657 + -2.4 389,127 - -4.0 389,135 + -1.3
Or see this region's nucleotide sequence