Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01770 and MMP_RS01775 are separated by 341 nucleotides MMP_RS01775 and MMP_RS01780 are separated by 160 nucleotides MMP_RS01780 and MMP_RS01785 are separated by 70 nucleotides
MMP_RS01770: MMP_RS01770 - ATP-dependent endonuclease, at 328,459 to 330,339
_RS01770
MMP_RS01775: MMP_RS01775 - TIGR00297 family protein, at 330,681 to 331,394
_RS01775
MMP_RS01780: MMP_RS01780 - TrmJ/YjtD family RNA methyltransferase, at 331,555 to 332,259
_RS01780
MMP_RS01785: MMP_RS01785 - hypothetical protein, at 332,330 to 332,743
_RS01785
Position (kb) 330
331
332 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 329.863 kb on + strand, within MMP_RS01770 at 329.866 kb on - strand, within MMP_RS01770 at 329.867 kb on + strand, within MMP_RS01770 at 329.915 kb on + strand, within MMP_RS01770 at 329.915 kb on + strand, within MMP_RS01770 at 329.915 kb on + strand, within MMP_RS01770 at 329.931 kb on - strand, within MMP_RS01770 at 329.946 kb on - strand, within MMP_RS01770 at 329.946 kb on - strand, within MMP_RS01770 at 329.950 kb on - strand, within MMP_RS01770 at 329.951 kb on + strand, within MMP_RS01770 at 329.952 kb on - strand, within MMP_RS01770 at 329.952 kb on - strand, within MMP_RS01770 at 329.952 kb on - strand, within MMP_RS01770 at 329.953 kb on + strand, within MMP_RS01770 at 329.953 kb on + strand, within MMP_RS01770 at 330.035 kb on - strand, within MMP_RS01770 at 330.036 kb on + strand, within MMP_RS01770 at 330.119 kb on - strand, within MMP_RS01770 at 330.120 kb on + strand, within MMP_RS01770 at 330.154 kb on - strand at 330.154 kb on - strand at 330.158 kb on - strand at 330.158 kb on - strand at 330.159 kb on + strand at 330.162 kb on + strand at 330.199 kb on - strand at 330.200 kb on + strand at 330.204 kb on - strand at 330.245 kb on - strand at 330.246 kb on + strand at 330.246 kb on + strand at 330.271 kb on - strand at 330.272 kb on + strand at 330.279 kb on - strand at 330.300 kb on + strand at 330.337 kb on - strand at 330.477 kb on - strand at 330.493 kb on + strand at 330.496 kb on - strand at 330.506 kb on - strand at 330.527 kb on - strand at 330.538 kb on - strand at 330.598 kb on + strand at 330.599 kb on - strand at 330.603 kb on + strand at 330.604 kb on - strand at 330.604 kb on - strand at 330.628 kb on + strand at 330.756 kb on - strand, within MMP_RS01775 at 330.967 kb on - strand, within MMP_RS01775 at 331.348 kb on - strand at 331.372 kb on - strand at 331.388 kb on + strand at 331.388 kb on + strand at 331.439 kb on - strand at 331.439 kb on - strand at 331.463 kb on + strand at 331.471 kb on + strand at 331.499 kb on + strand at 331.503 kb on - strand at 331.504 kb on + strand at 331.510 kb on + strand at 331.806 kb on - strand, within MMP_RS01780 at 331.831 kb on + strand, within MMP_RS01780 at 331.884 kb on + strand, within MMP_RS01780 at 332.120 kb on - strand, within MMP_RS01780 at 332.146 kb on - strand, within MMP_RS01780 at 332.159 kb on - strand, within MMP_RS01780 at 332.159 kb on - strand, within MMP_RS01780 at 332.159 kb on - strand, within MMP_RS01780 at 332.160 kb on + strand, within MMP_RS01780 at 332.228 kb on - strand at 332.245 kb on + strand at 332.258 kb on + strand at 332.294 kb on + strand at 332.296 kb on - strand at 332.336 kb on + strand at 332.337 kb on - strand at 332.372 kb on - strand, within MMP_RS01785
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 329,863 + MMP_RS01770 0.75 -0.3 329,866 - MMP_RS01770 0.75 -0.2 329,867 + MMP_RS01770 0.75 +0.0 329,915 + MMP_RS01770 0.77 +0.3 329,915 + MMP_RS01770 0.77 +0.5 329,915 + MMP_RS01770 0.77 +0.3 329,931 - MMP_RS01770 0.78 +0.2 329,946 - MMP_RS01770 0.79 +0.9 329,946 - MMP_RS01770 0.79 +0.7 329,950 - MMP_RS01770 0.79 +0.5 329,951 + MMP_RS01770 0.79 -0.1 329,952 - MMP_RS01770 0.79 -0.3 329,952 - MMP_RS01770 0.79 +0.1 329,952 - MMP_RS01770 0.79 +0.2 329,953 + MMP_RS01770 0.79 +0.7 329,953 + MMP_RS01770 0.79 +0.5 330,035 - MMP_RS01770 0.84 +0.7 330,036 + MMP_RS01770 0.84 -0.3 330,119 - MMP_RS01770 0.88 +0.4 330,120 + MMP_RS01770 0.88 -0.7 330,154 - +0.0 330,154 - -0.8 330,158 - +0.3 330,158 - +0.5 330,159 + +0.7 330,162 + +0.1 330,199 - +0.1 330,200 + +0.7 330,204 - +0.6 330,245 - +0.4 330,246 + -0.1 330,246 + +0.1 330,271 - -0.5 330,272 + +0.1 330,279 - +0.2 330,300 + +0.3 330,337 - +0.6 330,477 - +0.4 330,493 + -0.5 330,496 - +0.6 330,506 - +0.2 330,527 - -0.0 330,538 - -0.1 330,598 + +0.2 330,599 - -0.9 330,603 + +0.0 330,604 - +0.4 330,604 - -0.3 330,628 + +0.2 330,756 - MMP_RS01775 0.11 -2.2 330,967 - MMP_RS01775 0.40 -3.0 331,348 - -2.4 331,372 - -2.1 331,388 + -1.2 331,388 + -1.4 331,439 - +0.8 331,439 - +0.1 331,463 + -0.9 331,471 + +0.5 331,499 + +0.6 331,503 - +0.1 331,504 + -0.4 331,510 + +0.2 331,806 - MMP_RS01780 0.36 -0.4 331,831 + MMP_RS01780 0.39 -0.2 331,884 + MMP_RS01780 0.47 +0.1 332,120 - MMP_RS01780 0.80 +0.7 332,146 - MMP_RS01780 0.84 +0.4 332,159 - MMP_RS01780 0.86 -0.0 332,159 - MMP_RS01780 0.86 -0.6 332,159 - MMP_RS01780 0.86 -1.7 332,160 + MMP_RS01780 0.86 +0.2 332,228 - -0.2 332,245 + +0.6 332,258 + -0.1 332,294 + +0.4 332,296 - +0.4 332,336 + +0.3 332,337 - +0.5 332,372 - MMP_RS01785 0.10 +0.6
Or see this region's nucleotide sequence