Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01495 and MMP_RS01500 are separated by 75 nucleotides MMP_RS01500 and MMP_RS01505 are separated by 12 nucleotides MMP_RS01505 and MMP_RS01510 are separated by 20 nucleotides MMP_RS01510 and MMP_RS01515 are separated by 60 nucleotides
MMP_RS01495: MMP_RS01495 - TraB/GumN family protein, at 278,367 to 279,509
_RS01495
MMP_RS01500: MMP_RS01500 - CBS domain-containing protein, at 279,585 to 280,046
_RS01500
MMP_RS01505: MMP_RS01505 - hypothetical protein, at 280,059 to 281,036
_RS01505
MMP_RS01510: MMP_RS01510 - phosphoribosyl-AMP cyclohydrolase, at 281,057 to 281,446
_RS01510
MMP_RS01515: MMP_RS01515 - PINc/VapC family ATPase, at 281,507 to 283,399
_RS01515
Position (kb) 280
281
282 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 279.264 kb on - strand, within MMP_RS01495 at 279.283 kb on - strand, within MMP_RS01495 at 279.283 kb on - strand, within MMP_RS01495 at 279.501 kb on - strand at 279.501 kb on - strand at 279.504 kb on + strand at 279.504 kb on + strand at 279.504 kb on + strand at 279.505 kb on - strand at 279.512 kb on - strand at 279.521 kb on - strand at 279.523 kb on - strand at 279.524 kb on + strand at 279.524 kb on + strand at 279.527 kb on - strand at 279.527 kb on - strand at 279.528 kb on + strand at 279.531 kb on + strand at 279.535 kb on + strand at 279.535 kb on + strand at 279.551 kb on + strand at 279.551 kb on + strand at 279.552 kb on - strand at 279.552 kb on - strand at 279.552 kb on - strand at 279.552 kb on - strand at 279.552 kb on - strand at 279.552 kb on - strand at 279.553 kb on + strand at 279.554 kb on - strand at 279.554 kb on - strand at 279.555 kb on + strand at 279.555 kb on + strand at 279.556 kb on - strand at 279.556 kb on - strand at 279.557 kb on + strand at 279.557 kb on + strand at 279.656 kb on + strand, within MMP_RS01500 at 279.913 kb on - strand, within MMP_RS01500 at 279.914 kb on + strand, within MMP_RS01500 at 280.934 kb on - strand at 280.991 kb on - strand at 280.991 kb on - strand at 280.993 kb on - strand at 280.994 kb on + strand at 281.301 kb on - strand at 281.374 kb on - strand at 281.375 kb on + strand at 281.375 kb on + strand at 281.617 kb on + strand at 281.742 kb on - strand, within MMP_RS01515 at 281.742 kb on - strand, within MMP_RS01515 at 281.767 kb on + strand, within MMP_RS01515 at 281.767 kb on + strand, within MMP_RS01515 at 281.770 kb on + strand, within MMP_RS01515 at 281.781 kb on + strand, within MMP_RS01515 at 281.924 kb on + strand, within MMP_RS01515 at 281.974 kb on + strand, within MMP_RS01515
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 279,264 - MMP_RS01495 0.78 -4.2 279,283 - MMP_RS01495 0.80 -3.9 279,283 - MMP_RS01495 0.80 -4.6 279,501 - -3.6 279,501 - -2.8 279,504 + -5.9 279,504 + -5.6 279,504 + -3.7 279,505 - -3.7 279,512 - -4.5 279,521 - -0.3 279,523 - -0.4 279,524 + -0.3 279,524 + -0.4 279,527 - -0.5 279,527 - -0.8 279,528 + -0.7 279,531 + -0.5 279,535 + -2.4 279,535 + -3.3 279,551 + -3.2 279,551 + -5.7 279,552 - -3.1 279,552 - -5.4 279,552 - -5.3 279,552 - -1.5 279,552 - -1.6 279,552 - -4.8 279,553 + -3.6 279,554 - -4.3 279,554 - -4.1 279,555 + -4.4 279,555 + -5.3 279,556 - -4.4 279,556 - -4.6 279,557 + -4.2 279,557 + -2.5 279,656 + MMP_RS01500 0.15 -3.7 279,913 - MMP_RS01500 0.71 -3.5 279,914 + MMP_RS01500 0.71 -4.1 280,934 - -3.0 280,991 - -3.2 280,991 - -3.3 280,993 - -3.5 280,994 + -3.4 281,301 - -1.4 281,374 - -2.1 281,375 + -3.2 281,375 + -3.1 281,617 + +0.4 281,742 - MMP_RS01515 0.12 -0.1 281,742 - MMP_RS01515 0.12 +0.2 281,767 + MMP_RS01515 0.14 -0.1 281,767 + MMP_RS01515 0.14 +0.3 281,770 + MMP_RS01515 0.14 -0.2 281,781 + MMP_RS01515 0.14 +0.9 281,924 + MMP_RS01515 0.22 +0.8 281,974 + MMP_RS01515 0.25 -1.3
Or see this region's nucleotide sequence