Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01450 and CCNA_R0036 are separated by 129 nucleotides CCNA_R0036 and CCNA_R0037 are separated by 45 nucleotides CCNA_R0037 and CCNA_01451 are separated by 172 nucleotides CCNA_01451 and CCNA_01452 overlap by 32 nucleotides
CCNA_01450: CCNA_01450 - single-stranded-DNA-specific exonuclease recJ, at 1,562,926 to 1,564,746
_01450
CCNA_R0036: CCNA_R0036 - tRNA-Glu, at 1,564,876 to 1,564,950
_R0036
CCNA_R0037: CCNA_R0037 - tRNA-Glu, at 1,564,996 to 1,565,070
_R0037
CCNA_01451: CCNA_01451 - cold shock protein cspD, at 1,565,243 to 1,565,833
_01451
CCNA_01452: CCNA_01452 - hypothetical protein, at 1,565,802 to 1,566,356
_01452
Position (kb)
1564
1565
1566 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1564.741 kb on + strand at 1564.763 kb on + strand at 1564.763 kb on + strand at 1564.774 kb on + strand at 1564.778 kb on + strand at 1564.779 kb on + strand at 1565.020 kb on + strand, within CCNA_R0037 at 1565.045 kb on + strand, within CCNA_R0037 at 1565.055 kb on + strand, within CCNA_R0037 at 1565.151 kb on - strand at 1565.223 kb on + strand at 1565.228 kb on + strand at 1565.236 kb on - strand at 1565.236 kb on - strand at 1565.236 kb on - strand at 1565.324 kb on + strand, within CCNA_01451 at 1565.329 kb on + strand, within CCNA_01451 at 1565.330 kb on + strand, within CCNA_01451 at 1565.330 kb on + strand, within CCNA_01451 at 1565.338 kb on - strand, within CCNA_01451 at 1565.355 kb on + strand, within CCNA_01451 at 1565.355 kb on + strand, within CCNA_01451 at 1565.355 kb on + strand, within CCNA_01451 at 1565.355 kb on + strand, within CCNA_01451 at 1565.360 kb on + strand, within CCNA_01451 at 1565.363 kb on - strand, within CCNA_01451 at 1565.363 kb on - strand, within CCNA_01451 at 1565.378 kb on - strand, within CCNA_01451 at 1565.434 kb on - strand, within CCNA_01451 at 1565.468 kb on + strand, within CCNA_01451 at 1565.511 kb on - strand, within CCNA_01451 at 1565.533 kb on - strand, within CCNA_01451 at 1565.604 kb on - strand, within CCNA_01451 at 1565.610 kb on + strand, within CCNA_01451 at 1565.635 kb on - strand, within CCNA_01451 at 1565.662 kb on + strand, within CCNA_01451 at 1565.663 kb on + strand, within CCNA_01451 at 1565.663 kb on + strand, within CCNA_01451 at 1565.663 kb on - strand, within CCNA_01451 at 1565.664 kb on - strand, within CCNA_01451 at 1565.698 kb on - strand, within CCNA_01451 at 1565.698 kb on - strand, within CCNA_01451 at 1565.765 kb on + strand, within CCNA_01451 at 1565.765 kb on + strand, within CCNA_01451 at 1565.787 kb on + strand at 1565.833 kb on + strand at 1565.845 kb on + strand at 1565.925 kb on + strand, within CCNA_01452 at 1565.934 kb on + strand, within CCNA_01452 at 1565.935 kb on + strand, within CCNA_01452 at 1565.943 kb on - strand, within CCNA_01452 at 1565.962 kb on - strand, within CCNA_01452 at 1565.981 kb on + strand, within CCNA_01452 at 1566.023 kb on + strand, within CCNA_01452 at 1566.031 kb on - strand, within CCNA_01452 at 1566.056 kb on + strand, within CCNA_01452
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 1,564,741 + -0.2 1,564,763 + +2.4 1,564,763 + +0.6 1,564,774 + -0.2 1,564,778 + +1.9 1,564,779 + +1.1 1,565,020 + CCNA_R0037 0.32 +4.8 1,565,045 + CCNA_R0037 0.65 +4.6 1,565,055 + CCNA_R0037 0.79 +3.7 1,565,151 - -1.2 1,565,223 + +0.8 1,565,228 + +0.8 1,565,236 - -0.3 1,565,236 - +0.1 1,565,236 - +0.2 1,565,324 + CCNA_01451 0.14 -1.7 1,565,329 + CCNA_01451 0.15 -0.3 1,565,330 + CCNA_01451 0.15 -0.8 1,565,330 + CCNA_01451 0.15 -1.5 1,565,338 - CCNA_01451 0.16 +3.0 1,565,355 + CCNA_01451 0.19 -1.3 1,565,355 + CCNA_01451 0.19 -1.2 1,565,355 + CCNA_01451 0.19 -0.1 1,565,355 + CCNA_01451 0.19 +0.9 1,565,360 + CCNA_01451 0.20 +1.3 1,565,363 - CCNA_01451 0.20 +1.0 1,565,363 - CCNA_01451 0.20 -0.5 1,565,378 - CCNA_01451 0.23 -0.9 1,565,434 - CCNA_01451 0.32 -1.3 1,565,468 + CCNA_01451 0.38 -0.3 1,565,511 - CCNA_01451 0.45 +1.1 1,565,533 - CCNA_01451 0.49 -1.0 1,565,604 - CCNA_01451 0.61 +2.7 1,565,610 + CCNA_01451 0.62 +1.5 1,565,635 - CCNA_01451 0.66 +0.2 1,565,662 + CCNA_01451 0.71 +1.3 1,565,663 + CCNA_01451 0.71 +1.1 1,565,663 + CCNA_01451 0.71 +0.1 1,565,663 - CCNA_01451 0.71 +1.0 1,565,664 - CCNA_01451 0.71 +0.1 1,565,698 - CCNA_01451 0.77 +1.2 1,565,698 - CCNA_01451 0.77 +1.5 1,565,765 + CCNA_01451 0.88 +0.2 1,565,765 + CCNA_01451 0.88 +1.1 1,565,787 + +0.1 1,565,833 + +1.2 1,565,845 + +0.6 1,565,925 + CCNA_01452 0.22 +0.3 1,565,934 + CCNA_01452 0.24 +4.1 1,565,935 + CCNA_01452 0.24 +1.6 1,565,943 - CCNA_01452 0.25 -0.1 1,565,962 - CCNA_01452 0.29 -0.8 1,565,981 + CCNA_01452 0.32 +1.0 1,566,023 + CCNA_01452 0.40 +0.4 1,566,031 - CCNA_01452 0.41 -1.2 1,566,056 + CCNA_01452 0.46 +0.1
Or see this region's nucleotide sequence