Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03876 and CCNA_03877 are separated by 258 nucleotides CCNA_03877 and CCNA_03878 overlap by 52 nucleotides CCNA_03878 and CCNA_03879 overlap by 4 nucleotides
CCNA_03876: CCNA_03876 - transcription termination factor rho, at 4,038,433 to 4,039,866
_03876
CCNA_03877: CCNA_03877 - conserved hypothetical membrane spanning protein, at 4,040,125 to 4,040,640
_03877
CCNA_03878: CCNA_03878 - ferrochelatase, at 4,040,589 to 4,041,632
_03878
CCNA_03879: CCNA_03879 - uroporphyrinogen decarboxylase, at 4,041,629 to 4,042,684
_03879
Position (kb)
4040
4041
4042 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4039.609 kb on - strand, within CCNA_03876 at 4039.874 kb on - strand at 4039.881 kb on - strand at 4039.900 kb on - strand at 4039.961 kb on - strand at 4039.967 kb on + strand at 4040.017 kb on + strand at 4040.045 kb on + strand at 4040.116 kb on + strand at 4040.138 kb on - strand at 4040.147 kb on - strand at 4040.606 kb on - strand at 4040.625 kb on - strand at 4040.625 kb on - strand at 4040.640 kb on - strand at 4040.640 kb on - strand at 4040.664 kb on - strand at 4040.706 kb on - strand, within CCNA_03878 at 4041.640 kb on + strand at 4041.640 kb on + strand at 4041.640 kb on + strand at 4041.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 4,039,609 - CCNA_03876 0.82 +0.7 4,039,874 - +3.2 4,039,881 - +1.6 4,039,900 - -0.2 4,039,961 - -0.6 4,039,967 + +1.8 4,040,017 + +0.8 4,040,045 + -0.0 4,040,116 + -0.2 4,040,138 - +1.6 4,040,147 - +3.0 4,040,606 - +0.4 4,040,625 - +0.7 4,040,625 - +1.1 4,040,640 - +1.7 4,040,640 - +1.9 4,040,664 - +0.3 4,040,706 - CCNA_03878 0.11 -2.1 4,041,640 + -1.7 4,041,640 + -1.1 4,041,640 + -1.5 4,041,657 + -1.1
Or see this region's nucleotide sequence