Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02081 and CCNA_02082 are separated by 84 nucleotides CCNA_02082 and CCNA_02083 are separated by 133 nucleotides CCNA_02083 and CCNA_02084 overlap by 4 nucleotides CCNA_02084 and CCNA_02085 overlap by 4 nucleotides
CCNA_02081: CCNA_02081 - Sec-independent protein translocase protein tatB, at 2,231,825 to 2,232,427
_02081
CCNA_02082: CCNA_02082 - Sec-independent protein translocase protein tatA, at 2,232,512 to 2,232,736
_02082
CCNA_02083: CCNA_02083 - chromosome segregation and condensation protein B scpB, at 2,232,870 to 2,233,535
_02083
CCNA_02084: CCNA_02084 - chromosome segregation and condensation protein fusion scpA, at 2,233,532 to 2,234,356
_02084
CCNA_02085: CCNA_02085 - anhydromuramoyl-peptide exo-beta-N-acetylglucosaminidase, at 2,234,353 to 2,235,390
_02085
Position (kb)
2232
2233
2234 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2231.892 kb on + strand, within CCNA_02081 at 2231.900 kb on - strand, within CCNA_02081 at 2231.900 kb on - strand, within CCNA_02081 at 2231.954 kb on - strand, within CCNA_02081 at 2231.969 kb on - strand, within CCNA_02081 at 2231.992 kb on - strand, within CCNA_02081 at 2232.023 kb on - strand, within CCNA_02081 at 2232.023 kb on - strand, within CCNA_02081 at 2232.096 kb on + strand, within CCNA_02081 at 2232.101 kb on + strand, within CCNA_02081 at 2232.137 kb on - strand, within CCNA_02081 at 2232.290 kb on - strand, within CCNA_02081 at 2232.443 kb on - strand at 2232.443 kb on - strand at 2232.443 kb on - strand at 2232.458 kb on - strand at 2232.511 kb on - strand at 2232.749 kb on + strand at 2232.764 kb on + strand at 2232.772 kb on - strand at 2232.772 kb on - strand at 2232.772 kb on - strand at 2232.794 kb on - strand at 2232.810 kb on - strand at 2232.821 kb on + strand at 2232.829 kb on - strand at 2232.834 kb on - strand at 2232.960 kb on + strand, within CCNA_02083 at 2232.961 kb on - strand, within CCNA_02083 at 2232.968 kb on - strand, within CCNA_02083 at 2232.975 kb on + strand, within CCNA_02083 at 2233.067 kb on - strand, within CCNA_02083 at 2233.077 kb on - strand, within CCNA_02083 at 2233.077 kb on - strand, within CCNA_02083 at 2233.128 kb on - strand, within CCNA_02083 at 2233.146 kb on + strand, within CCNA_02083 at 2233.167 kb on + strand, within CCNA_02083 at 2233.207 kb on + strand, within CCNA_02083 at 2233.215 kb on - strand, within CCNA_02083 at 2233.221 kb on - strand, within CCNA_02083 at 2233.221 kb on - strand, within CCNA_02083 at 2233.270 kb on + strand, within CCNA_02083 at 2233.270 kb on + strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.278 kb on - strand, within CCNA_02083 at 2233.362 kb on + strand, within CCNA_02083 at 2233.370 kb on - strand, within CCNA_02083 at 2233.375 kb on + strand, within CCNA_02083 at 2233.449 kb on - strand, within CCNA_02083 at 2233.493 kb on - strand at 2233.520 kb on + strand at 2233.520 kb on + strand at 2233.585 kb on - strand at 2233.595 kb on - strand at 2233.595 kb on - strand at 2233.622 kb on - strand, within CCNA_02084 at 2233.645 kb on + strand, within CCNA_02084 at 2233.724 kb on - strand, within CCNA_02084 at 2233.736 kb on - strand, within CCNA_02084 at 2233.741 kb on - strand, within CCNA_02084 at 2233.767 kb on - strand, within CCNA_02084 at 2233.856 kb on + strand, within CCNA_02084 at 2233.864 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.883 kb on - strand, within CCNA_02084 at 2233.906 kb on - strand, within CCNA_02084 at 2233.907 kb on - strand, within CCNA_02084 at 2233.909 kb on + strand, within CCNA_02084 at 2233.909 kb on + strand, within CCNA_02084 at 2233.917 kb on - strand, within CCNA_02084 at 2233.925 kb on + strand, within CCNA_02084 at 2233.995 kb on - strand, within CCNA_02084 at 2234.049 kb on + strand, within CCNA_02084 at 2234.049 kb on + strand, within CCNA_02084 at 2234.057 kb on - strand, within CCNA_02084 at 2234.072 kb on - strand, within CCNA_02084 at 2234.072 kb on - strand, within CCNA_02084 at 2234.072 kb on - strand, within CCNA_02084 at 2234.091 kb on + strand, within CCNA_02084 at 2234.094 kb on - strand, within CCNA_02084 at 2234.117 kb on - strand, within CCNA_02084 at 2234.117 kb on - strand, within CCNA_02084 at 2234.169 kb on + strand, within CCNA_02084 at 2234.219 kb on - strand, within CCNA_02084 at 2234.226 kb on - strand, within CCNA_02084 at 2234.265 kb on - strand, within CCNA_02084 at 2234.268 kb on - strand, within CCNA_02084 at 2234.327 kb on + strand at 2234.395 kb on + strand at 2234.403 kb on - strand at 2234.437 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,231,892 + CCNA_02081 0.11 +0.7 2,231,900 - CCNA_02081 0.12 +3.2 2,231,900 - CCNA_02081 0.12 +2.3 2,231,954 - CCNA_02081 0.21 +3.6 2,231,969 - CCNA_02081 0.24 +3.9 2,231,992 - CCNA_02081 0.28 +3.4 2,232,023 - CCNA_02081 0.33 +2.1 2,232,023 - CCNA_02081 0.33 +1.9 2,232,096 + CCNA_02081 0.45 -0.4 2,232,101 + CCNA_02081 0.46 -0.6 2,232,137 - CCNA_02081 0.52 +2.3 2,232,290 - CCNA_02081 0.77 +3.9 2,232,443 - +2.9 2,232,443 - +3.7 2,232,443 - +2.8 2,232,458 - -0.8 2,232,511 - +3.3 2,232,749 + -1.1 2,232,764 + -0.6 2,232,772 - +0.9 2,232,772 - +2.7 2,232,772 - +1.1 2,232,794 - +1.0 2,232,810 - -1.0 2,232,821 + -0.3 2,232,829 - -0.9 2,232,834 - +1.2 2,232,960 + CCNA_02083 0.14 +0.2 2,232,961 - CCNA_02083 0.14 +0.1 2,232,968 - CCNA_02083 0.15 +0.1 2,232,975 + CCNA_02083 0.16 -0.9 2,233,067 - CCNA_02083 0.30 -0.2 2,233,077 - CCNA_02083 0.31 +0.2 2,233,077 - CCNA_02083 0.31 +0.9 2,233,128 - CCNA_02083 0.39 -0.5 2,233,146 + CCNA_02083 0.41 +0.0 2,233,167 + CCNA_02083 0.45 +1.5 2,233,207 + CCNA_02083 0.51 -0.8 2,233,215 - CCNA_02083 0.52 +1.4 2,233,221 - CCNA_02083 0.53 +1.3 2,233,221 - CCNA_02083 0.53 +0.2 2,233,270 + CCNA_02083 0.60 +0.2 2,233,270 + CCNA_02083 0.60 +1.3 2,233,278 - CCNA_02083 0.61 +0.3 2,233,278 - CCNA_02083 0.61 -0.0 2,233,278 - CCNA_02083 0.61 -0.5 2,233,278 - CCNA_02083 0.61 +0.4 2,233,278 - CCNA_02083 0.61 -0.9 2,233,278 - CCNA_02083 0.61 +1.0 2,233,278 - CCNA_02083 0.61 -0.8 2,233,362 + CCNA_02083 0.74 +1.8 2,233,370 - CCNA_02083 0.75 +0.3 2,233,375 + CCNA_02083 0.76 +0.2 2,233,449 - CCNA_02083 0.87 +1.0 2,233,493 - +1.4 2,233,520 + +0.0 2,233,520 + +1.8 2,233,585 - -2.2 2,233,595 - +1.6 2,233,595 - -0.3 2,233,622 - CCNA_02084 0.11 +0.4 2,233,645 + CCNA_02084 0.14 +1.5 2,233,724 - CCNA_02084 0.23 -0.7 2,233,736 - CCNA_02084 0.25 -0.9 2,233,741 - CCNA_02084 0.25 -0.7 2,233,767 - CCNA_02084 0.28 -0.6 2,233,856 + CCNA_02084 0.39 +0.8 2,233,864 - CCNA_02084 0.40 +0.2 2,233,883 - CCNA_02084 0.43 -0.8 2,233,883 - CCNA_02084 0.43 -0.1 2,233,883 - CCNA_02084 0.43 +1.0 2,233,883 - CCNA_02084 0.43 +0.7 2,233,883 - CCNA_02084 0.43 -0.3 2,233,883 - CCNA_02084 0.43 -0.7 2,233,906 - CCNA_02084 0.45 +0.3 2,233,907 - CCNA_02084 0.45 -0.6 2,233,909 + CCNA_02084 0.46 +4.3 2,233,909 + CCNA_02084 0.46 +3.5 2,233,917 - CCNA_02084 0.47 -1.1 2,233,925 + CCNA_02084 0.48 -0.3 2,233,995 - CCNA_02084 0.56 +3.6 2,234,049 + CCNA_02084 0.63 +2.1 2,234,049 + CCNA_02084 0.63 +0.4 2,234,057 - CCNA_02084 0.64 +0.9 2,234,072 - CCNA_02084 0.65 +0.1 2,234,072 - CCNA_02084 0.65 -0.2 2,234,072 - CCNA_02084 0.65 +0.5 2,234,091 + CCNA_02084 0.68 -0.0 2,234,094 - CCNA_02084 0.68 +2.8 2,234,117 - CCNA_02084 0.71 -2.6 2,234,117 - CCNA_02084 0.71 +0.5 2,234,169 + CCNA_02084 0.77 +0.8 2,234,219 - CCNA_02084 0.83 +0.7 2,234,226 - CCNA_02084 0.84 +0.7 2,234,265 - CCNA_02084 0.89 +1.3 2,234,268 - CCNA_02084 0.89 +1.7 2,234,327 + +0.5 2,234,395 + -0.2 2,234,403 - +0.8 2,234,437 + +0.9
Or see this region's nucleotide sequence