Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02076 and CCNA_02077 overlap by 4 nucleotides CCNA_02077 and CCNA_02078 overlap by 4 nucleotides CCNA_02078 and CCNA_03896 are separated by 366 nucleotides
CCNA_02076: CCNA_02076 - protein-L-isoaspartate O-methyltransferase, at 2,226,974 to 2,227,642
_02076
CCNA_02077: CCNA_02077 - 5'(3')-nucleotidase/polyphosphatase SurE, at 2,227,639 to 2,228,430
_02077
CCNA_02078: CCNA_02078 - seryl-tRNA synthetase, at 2,228,427 to 2,229,875
_02078
CCNA_03896: CCNA_03896 - hypothetical protein, at 2,230,242 to 2,230,709
_03896
Position (kb)
2228
2229
2230 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2227.602 kb on + strand at 2227.610 kb on - strand at 2227.610 kb on - strand at 2227.687 kb on - strand at 2227.697 kb on + strand at 2227.845 kb on + strand, within CCNA_02077 at 2227.870 kb on + strand, within CCNA_02077 at 2227.955 kb on + strand, within CCNA_02077 at 2228.007 kb on - strand, within CCNA_02077 at 2228.007 kb on - strand, within CCNA_02077 at 2228.131 kb on - strand, within CCNA_02077 at 2228.221 kb on + strand, within CCNA_02077 at 2228.221 kb on + strand, within CCNA_02077 at 2228.285 kb on + strand, within CCNA_02077 at 2228.293 kb on - strand, within CCNA_02077 at 2228.374 kb on - strand at 2228.386 kb on - strand at 2230.116 kb on - strand at 2230.116 kb on - strand at 2230.116 kb on - strand at 2230.147 kb on - strand at 2230.184 kb on - strand at 2230.224 kb on + strand at 2230.232 kb on - strand at 2230.241 kb on - strand at 2230.280 kb on - strand at 2230.316 kb on + strand, within CCNA_03896 at 2230.318 kb on - strand, within CCNA_03896 at 2230.349 kb on + strand, within CCNA_03896 at 2230.351 kb on + strand, within CCNA_03896 at 2230.357 kb on - strand, within CCNA_03896 at 2230.382 kb on - strand, within CCNA_03896 at 2230.394 kb on + strand, within CCNA_03896 at 2230.415 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.456 kb on - strand, within CCNA_03896 at 2230.511 kb on + strand, within CCNA_03896 at 2230.511 kb on + strand, within CCNA_03896 at 2230.511 kb on - strand, within CCNA_03896 at 2230.522 kb on + strand, within CCNA_03896 at 2230.547 kb on + strand, within CCNA_03896 at 2230.565 kb on + strand, within CCNA_03896 at 2230.573 kb on - strand, within CCNA_03896 at 2230.674 kb on + strand at 2230.676 kb on - strand at 2230.680 kb on + strand at 2230.688 kb on - strand at 2230.688 kb on - strand at 2230.699 kb on - strand at 2230.715 kb on + strand at 2230.796 kb on - strand at 2230.844 kb on + strand at 2230.849 kb on + strand at 2230.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,227,602 + +1.0 2,227,610 - +2.0 2,227,610 - +3.4 2,227,687 - +3.0 2,227,697 + +1.4 2,227,845 + CCNA_02077 0.26 -1.8 2,227,870 + CCNA_02077 0.29 -0.6 2,227,955 + CCNA_02077 0.40 -0.1 2,228,007 - CCNA_02077 0.46 +3.0 2,228,007 - CCNA_02077 0.46 +3.3 2,228,131 - CCNA_02077 0.62 +3.2 2,228,221 + CCNA_02077 0.73 +0.7 2,228,221 + CCNA_02077 0.73 -0.3 2,228,285 + CCNA_02077 0.82 -0.9 2,228,293 - CCNA_02077 0.83 -0.1 2,228,374 - +3.5 2,228,386 - +3.1 2,230,116 - +1.3 2,230,116 - -0.2 2,230,116 - -1.2 2,230,147 - -1.5 2,230,184 - -1.4 2,230,224 + +1.6 2,230,232 - -0.0 2,230,241 - +0.5 2,230,280 - -0.5 2,230,316 + CCNA_03896 0.16 +0.3 2,230,318 - CCNA_03896 0.16 +1.3 2,230,349 + CCNA_03896 0.23 +2.4 2,230,351 + CCNA_03896 0.23 +1.8 2,230,357 - CCNA_03896 0.25 +0.1 2,230,382 - CCNA_03896 0.30 -0.7 2,230,394 + CCNA_03896 0.32 +1.5 2,230,415 + CCNA_03896 0.37 +2.4 2,230,422 + CCNA_03896 0.38 +1.1 2,230,422 + CCNA_03896 0.38 +3.2 2,230,422 + CCNA_03896 0.38 +3.0 2,230,422 + CCNA_03896 0.38 +1.8 2,230,430 - CCNA_03896 0.40 -1.6 2,230,430 - CCNA_03896 0.40 -1.1 2,230,430 - CCNA_03896 0.40 -1.6 2,230,430 - CCNA_03896 0.40 -0.3 2,230,430 - CCNA_03896 0.40 -1.4 2,230,430 - CCNA_03896 0.40 +1.4 2,230,430 - CCNA_03896 0.40 -1.5 2,230,430 - CCNA_03896 0.40 -0.2 2,230,456 - CCNA_03896 0.46 +0.5 2,230,511 + CCNA_03896 0.57 +2.6 2,230,511 + CCNA_03896 0.57 +2.5 2,230,511 - CCNA_03896 0.57 +0.1 2,230,522 + CCNA_03896 0.60 +2.7 2,230,547 + CCNA_03896 0.65 +2.9 2,230,565 + CCNA_03896 0.69 +3.4 2,230,573 - CCNA_03896 0.71 -0.9 2,230,674 + +3.4 2,230,676 - -1.5 2,230,680 + +3.6 2,230,688 - -2.3 2,230,688 - -0.5 2,230,699 - +0.1 2,230,715 + +3.6 2,230,796 - +0.6 2,230,844 + -0.4 2,230,849 + -0.1 2,230,852 - +1.1
Or see this region's nucleotide sequence