Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01999 and CCNA_02000 are separated by 139 nucleotides CCNA_02000 and CCNA_02001 are separated by 312 nucleotides CCNA_02001 and CCNA_02002 are separated by 12 nucleotides
CCNA_01999: CCNA_01999 - translation elongation factor Ts (EF-Ts), at 2,145,250 to 2,146,188
_01999
CCNA_02000: CCNA_02000 - SSU ribosomal protein S2P, at 2,146,328 to 2,147,134
_02000
CCNA_02001: CCNA_02001 - phosphoserine phosphatase, at 2,147,447 to 2,148,097
_02001
CCNA_02002: CCNA_02002 - methyltransferase, at 2,148,110 to 2,148,739
_02002
Position (kb)
2146
2147
2148 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2146.268 kb on - strand at 2146.273 kb on - strand at 2146.273 kb on - strand at 2146.273 kb on - strand at 2146.273 kb on - strand at 2146.427 kb on - strand, within CCNA_02000 at 2146.450 kb on - strand, within CCNA_02000 at 2147.423 kb on - strand at 2147.428 kb on - strand at 2147.440 kb on + strand at 2147.530 kb on - strand, within CCNA_02001 at 2147.532 kb on - strand, within CCNA_02001 at 2147.534 kb on + strand, within CCNA_02001 at 2147.564 kb on - strand, within CCNA_02001 at 2147.599 kb on - strand, within CCNA_02001 at 2147.618 kb on + strand, within CCNA_02001 at 2147.659 kb on - strand, within CCNA_02001 at 2147.731 kb on + strand, within CCNA_02001 at 2147.731 kb on + strand, within CCNA_02001 at 2147.749 kb on - strand, within CCNA_02001 at 2147.750 kb on - strand, within CCNA_02001 at 2147.803 kb on - strand, within CCNA_02001 at 2147.818 kb on + strand at 2147.826 kb on - strand, within CCNA_02001 at 2147.826 kb on - strand, within CCNA_02001 at 2147.826 kb on - strand, within CCNA_02001 at 2147.826 kb on - strand, within CCNA_02001 at 2147.826 kb on - strand, within CCNA_02001 at 2147.860 kb on + strand, within CCNA_02001 at 2147.865 kb on + strand, within CCNA_02001 at 2147.865 kb on + strand, within CCNA_02001 at 2147.865 kb on + strand, within CCNA_02001 at 2147.865 kb on + strand, within CCNA_02001 at 2147.865 kb on + strand, within CCNA_02001 at 2147.868 kb on - strand, within CCNA_02001 at 2147.873 kb on - strand, within CCNA_02001 at 2147.873 kb on - strand, within CCNA_02001 at 2147.873 kb on - strand, within CCNA_02001 at 2147.912 kb on + strand, within CCNA_02001 at 2147.923 kb on + strand, within CCNA_02001 at 2147.923 kb on + strand, within CCNA_02001 at 2147.924 kb on + strand, within CCNA_02001 at 2147.926 kb on - strand, within CCNA_02001 at 2147.951 kb on + strand, within CCNA_02001 at 2147.957 kb on + strand, within CCNA_02001 at 2147.957 kb on + strand, within CCNA_02001 at 2147.957 kb on + strand, within CCNA_02001 at 2147.979 kb on - strand, within CCNA_02001 at 2147.984 kb on - strand, within CCNA_02001 at 2148.050 kb on + strand at 2148.067 kb on + strand at 2148.086 kb on - strand at 2148.103 kb on + strand at 2148.112 kb on + strand at 2148.117 kb on + strand at 2148.120 kb on - strand at 2148.120 kb on - strand at 2148.125 kb on - strand at 2148.133 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,146,268 - +5.0 2,146,273 - +4.7 2,146,273 - +4.5 2,146,273 - +4.9 2,146,273 - +4.6 2,146,427 - CCNA_02000 0.12 +5.2 2,146,450 - CCNA_02000 0.15 +4.3 2,147,423 - -0.3 2,147,428 - -2.4 2,147,440 + +0.3 2,147,530 - CCNA_02001 0.13 -0.3 2,147,532 - CCNA_02001 0.13 +2.9 2,147,534 + CCNA_02001 0.13 -0.8 2,147,564 - CCNA_02001 0.18 +0.8 2,147,599 - CCNA_02001 0.23 -1.2 2,147,618 + CCNA_02001 0.26 +1.1 2,147,659 - CCNA_02001 0.33 -0.2 2,147,731 + CCNA_02001 0.44 -0.4 2,147,731 + CCNA_02001 0.44 +0.6 2,147,749 - CCNA_02001 0.46 -1.7 2,147,750 - CCNA_02001 0.47 +0.4 2,147,803 - CCNA_02001 0.55 -0.3 2,147,818 + +2.3 2,147,826 - CCNA_02001 0.58 -1.3 2,147,826 - CCNA_02001 0.58 +1.5 2,147,826 - CCNA_02001 0.58 +0.5 2,147,826 - CCNA_02001 0.58 +0.1 2,147,826 - CCNA_02001 0.58 -0.8 2,147,860 + CCNA_02001 0.63 -0.4 2,147,865 + CCNA_02001 0.64 +0.8 2,147,865 + CCNA_02001 0.64 +0.6 2,147,865 + CCNA_02001 0.64 -0.3 2,147,865 + CCNA_02001 0.64 +0.4 2,147,865 + CCNA_02001 0.64 +1.3 2,147,868 - CCNA_02001 0.65 +0.3 2,147,873 - CCNA_02001 0.65 -1.0 2,147,873 - CCNA_02001 0.65 -1.4 2,147,873 - CCNA_02001 0.65 -0.2 2,147,912 + CCNA_02001 0.71 +1.3 2,147,923 + CCNA_02001 0.73 +0.2 2,147,923 + CCNA_02001 0.73 -1.2 2,147,924 + CCNA_02001 0.73 +0.8 2,147,926 - CCNA_02001 0.74 -1.1 2,147,951 + CCNA_02001 0.77 +0.4 2,147,957 + CCNA_02001 0.78 -0.5 2,147,957 + CCNA_02001 0.78 -0.8 2,147,957 + CCNA_02001 0.78 -1.2 2,147,979 - CCNA_02001 0.82 -2.4 2,147,984 - CCNA_02001 0.82 +0.4 2,148,050 + -1.2 2,148,067 + -0.4 2,148,086 - -1.7 2,148,103 + -0.4 2,148,112 + +1.7 2,148,117 + +0.6 2,148,120 - +1.3 2,148,120 - +1.4 2,148,125 - -1.1 2,148,133 + +0.3
Or see this region's nucleotide sequence