Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01751 and CCNA_01752 are separated by 3 nucleotides CCNA_01752 and CCNA_01753 overlap by 8 nucleotides CCNA_01753 and CCNA_01754 are separated by 38 nucleotides
CCNA_01751: CCNA_01751 - aminodeoxychorismate lyase, at 1,878,709 to 1,879,809
_01751
CCNA_01752: CCNA_01752 - YicC-family protein, at 1,879,813 to 1,880,694
_01752
CCNA_01753: CCNA_01753 - guanylate kinase, at 1,880,687 to 1,881,328
_01753
CCNA_01754: CCNA_01754 - transglycosylase associated protein, at 1,881,367 to 1,881,672
_01754
Position (kb)
1879
1880
1881 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1878.849 kb on + strand, within CCNA_01751 at 1878.921 kb on + strand, within CCNA_01751 at 1878.956 kb on + strand, within CCNA_01751 at 1879.209 kb on + strand, within CCNA_01751 at 1879.235 kb on + strand, within CCNA_01751 at 1879.243 kb on + strand, within CCNA_01751 at 1879.243 kb on + strand, within CCNA_01751 at 1879.557 kb on + strand, within CCNA_01751 at 1879.565 kb on - strand, within CCNA_01751 at 1879.565 kb on - strand, within CCNA_01751 at 1879.649 kb on + strand, within CCNA_01751 at 1879.713 kb on - strand at 1879.824 kb on + strand at 1879.853 kb on - strand at 1879.983 kb on + strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1880.062 kb on + strand, within CCNA_01752 at 1880.121 kb on + strand, within CCNA_01752 at 1880.153 kb on - strand, within CCNA_01752 at 1880.153 kb on - strand, within CCNA_01752 at 1880.202 kb on + strand, within CCNA_01752 at 1880.334 kb on + strand, within CCNA_01752 at 1880.340 kb on + strand, within CCNA_01752 at 1880.342 kb on - strand, within CCNA_01752 at 1880.342 kb on - strand, within CCNA_01752 at 1880.376 kb on + strand, within CCNA_01752 at 1880.393 kb on + strand, within CCNA_01752 at 1880.393 kb on + strand, within CCNA_01752 at 1880.403 kb on + strand, within CCNA_01752 at 1880.479 kb on + strand, within CCNA_01752 at 1880.479 kb on + strand, within CCNA_01752 at 1880.487 kb on - strand, within CCNA_01752 at 1880.523 kb on + strand, within CCNA_01752 at 1880.523 kb on + strand, within CCNA_01752 at 1880.567 kb on + strand, within CCNA_01752 at 1880.586 kb on + strand, within CCNA_01752 at 1880.623 kb on - strand at 1880.623 kb on - strand at 1880.636 kb on + strand at 1880.644 kb on - strand at 1880.651 kb on - strand at 1880.659 kb on - strand at 1881.322 kb on + strand at 1881.322 kb on + strand at 1881.330 kb on - strand at 1881.330 kb on - strand at 1881.330 kb on - strand at 1881.330 kb on - strand at 1881.382 kb on + strand at 1881.553 kb on - strand, within CCNA_01754 at 1881.610 kb on + strand, within CCNA_01754 at 1881.625 kb on + strand, within CCNA_01754 at 1881.641 kb on - strand, within CCNA_01754 at 1881.660 kb on - strand at 1881.660 kb on - strand at 1881.660 kb on - strand at 1881.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 1,878,849 + CCNA_01751 0.13 +4.0 1,878,921 + CCNA_01751 0.19 +4.1 1,878,956 + CCNA_01751 0.22 +4.6 1,879,209 + CCNA_01751 0.45 +4.2 1,879,235 + CCNA_01751 0.48 +4.4 1,879,243 + CCNA_01751 0.49 +3.6 1,879,243 + CCNA_01751 0.49 +4.8 1,879,557 + CCNA_01751 0.77 +4.2 1,879,565 - CCNA_01751 0.78 +0.6 1,879,565 - CCNA_01751 0.78 -0.2 1,879,649 + CCNA_01751 0.85 +5.6 1,879,713 - +1.3 1,879,824 + +1.5 1,879,853 - +1.8 1,879,983 + CCNA_01752 0.19 +1.1 1,879,991 - CCNA_01752 0.20 +0.8 1,879,991 - CCNA_01752 0.20 -1.3 1,879,991 - CCNA_01752 0.20 -0.0 1,880,062 + CCNA_01752 0.28 +2.4 1,880,121 + CCNA_01752 0.35 +3.4 1,880,153 - CCNA_01752 0.39 +0.1 1,880,153 - CCNA_01752 0.39 +1.0 1,880,202 + CCNA_01752 0.44 +1.7 1,880,334 + CCNA_01752 0.59 +1.4 1,880,340 + CCNA_01752 0.60 +2.5 1,880,342 - CCNA_01752 0.60 -0.4 1,880,342 - CCNA_01752 0.60 +0.8 1,880,376 + CCNA_01752 0.64 +1.5 1,880,393 + CCNA_01752 0.66 +2.6 1,880,393 + CCNA_01752 0.66 +2.5 1,880,403 + CCNA_01752 0.67 +2.1 1,880,479 + CCNA_01752 0.76 +1.3 1,880,479 + CCNA_01752 0.76 +1.9 1,880,487 - CCNA_01752 0.76 +1.2 1,880,523 + CCNA_01752 0.80 +2.0 1,880,523 + CCNA_01752 0.80 +1.6 1,880,567 + CCNA_01752 0.85 +1.6 1,880,586 + CCNA_01752 0.88 +2.4 1,880,623 - -0.6 1,880,623 - +2.3 1,880,636 + +0.6 1,880,644 - -0.9 1,880,651 - -1.2 1,880,659 - +0.6 1,881,322 + +2.2 1,881,322 + +1.2 1,881,330 - +0.6 1,881,330 - +0.2 1,881,330 - +0.9 1,881,330 - -1.1 1,881,382 + +0.5 1,881,553 - CCNA_01754 0.61 -0.7 1,881,610 + CCNA_01754 0.79 +0.8 1,881,625 + CCNA_01754 0.84 +0.8 1,881,641 - CCNA_01754 0.90 +0.5 1,881,660 - +2.3 1,881,660 - +3.0 1,881,660 - +1.3 1,881,681 - -0.8
Or see this region's nucleotide sequence