Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01419 and CCNA_01420 are separated by 32 nucleotides CCNA_01420 and CCNA_01421 are separated by 65 nucleotides CCNA_01421 and CCNA_01422 overlap by 4 nucleotides CCNA_01422 and CCNA_01423 overlap by 41 nucleotides
CCNA_01419: CCNA_01419 - serine hydroxymethyltransferase, at 1,536,723 to 1,538,009
_01419
CCNA_01420: CCNA_01420 - NrdR family transcriptional regulator, at 1,538,042 to 1,538,515
_01420
CCNA_01421: CCNA_01421 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,538,581 to 1,539,045
_01421
CCNA_01422: CCNA_01422 - N utilization substance protein B NusB, at 1,539,042 to 1,539,491
_01422
CCNA_01423: CCNA_01423 - thiamine-monophosphate kinase, at 1,539,451 to 1,540,509
_01423
Position (kb)
1538
1539 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1538.032 kb on + strand at 1538.032 kb on + strand at 1538.095 kb on + strand, within CCNA_01420 at 1538.200 kb on + strand, within CCNA_01420 at 1538.261 kb on + strand, within CCNA_01420 at 1538.280 kb on + strand, within CCNA_01420 at 1538.331 kb on + strand, within CCNA_01420 at 1538.450 kb on + strand, within CCNA_01420 at 1538.467 kb on + strand, within CCNA_01420 at 1538.499 kb on + strand at 1538.509 kb on + strand at 1538.515 kb on - strand at 1538.515 kb on - strand at 1538.517 kb on - strand at 1538.523 kb on + strand at 1539.031 kb on + strand at 1539.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 1,538,032 + +3.1 1,538,032 + +3.0 1,538,095 + CCNA_01420 0.11 +1.6 1,538,200 + CCNA_01420 0.33 +3.3 1,538,261 + CCNA_01420 0.46 +2.4 1,538,280 + CCNA_01420 0.50 +2.8 1,538,331 + CCNA_01420 0.61 +2.6 1,538,450 + CCNA_01420 0.86 +3.7 1,538,467 + CCNA_01420 0.90 +2.8 1,538,499 + +2.4 1,538,509 + +1.6 1,538,515 - +0.5 1,538,515 - +2.7 1,538,517 - +0.7 1,538,523 + +1.9 1,539,031 + +3.0 1,539,044 - +0.2
Or see this region's nucleotide sequence