Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00917 and CCNA_00918 are separated by 73 nucleotides CCNA_00918 and CCNA_00919 are separated by 37 nucleotides CCNA_00919 and CCNA_00920 overlap by 4 nucleotides
CCNA_00917: CCNA_00917 - antitoxin protein parD-1, at 994,996 to 995,262
_00917
CCNA_00918: CCNA_00918 - peptide release factor-glutamine N5-methyltransferase, at 995,336 to 996,205
_00918
CCNA_00919: CCNA_00919 - hypothetical protein, at 996,243 to 996,467
_00919
CCNA_00920: CCNA_00920 - hypothetical protein, at 996,464 to 997,576
_00920
Position (kb) 996
997 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 995.339 kb on + strand at 995.339 kb on + strand at 996.210 kb on + strand at 996.347 kb on + strand, within CCNA_00919 at 996.413 kb on + strand, within CCNA_00919 at 996.422 kb on + strand, within CCNA_00919 at 996.432 kb on + strand, within CCNA_00919 at 996.697 kb on + strand, within CCNA_00920 at 996.697 kb on + strand, within CCNA_00920 at 996.697 kb on + strand, within CCNA_00920 at 996.760 kb on + strand, within CCNA_00920 at 996.760 kb on + strand, within CCNA_00920 at 996.808 kb on + strand, within CCNA_00920 at 997.013 kb on - strand, within CCNA_00920 at 997.146 kb on + strand, within CCNA_00920 at 997.195 kb on + strand, within CCNA_00920 at 997.211 kb on - strand, within CCNA_00920
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 995,339 + +1.7 995,339 + +1.8 996,210 + +3.1 996,347 + CCNA_00919 0.46 +3.1 996,413 + CCNA_00919 0.76 +2.5 996,422 + CCNA_00919 0.80 +1.3 996,432 + CCNA_00919 0.84 +2.3 996,697 + CCNA_00920 0.21 +1.6 996,697 + CCNA_00920 0.21 +2.7 996,697 + CCNA_00920 0.21 +2.4 996,760 + CCNA_00920 0.27 +3.2 996,760 + CCNA_00920 0.27 +3.7 996,808 + CCNA_00920 0.31 +3.3 997,013 - CCNA_00920 0.49 +0.6 997,146 + CCNA_00920 0.61 +3.9 997,195 + CCNA_00920 0.66 +4.6 997,211 - CCNA_00920 0.67 -2.0
Or see this region's nucleotide sequence