Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides CCNA_00227 and CCNA_00228 overlap by 1 nucleotides CCNA_00228 and CCNA_00229 are separated by 119 nucleotides
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
CCNA_00228: CCNA_00228 - putative membrane spanning protein, at 244,056 to 244,670
_00228
CCNA_00229: CCNA_00229 - CAAX amino terminal protease family protein, at 244,790 to 245,764
_00229
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227 at 243.708 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.751 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.767 kb on + strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.844 kb on + strand, within CCNA_00227 at 243.848 kb on - strand, within CCNA_00227 at 243.869 kb on + strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.939 kb on - strand, within CCNA_00227 at 243.955 kb on + strand, within CCNA_00227 at 243.956 kb on - strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 244.016 kb on + strand at 244.016 kb on + strand at 244.022 kb on + strand at 244.062 kb on - strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.068 kb on + strand at 244.068 kb on + strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.076 kb on - strand at 244.076 kb on - strand at 244.076 kb on - strand at 244.112 kb on - strand at 244.112 kb on - strand at 244.135 kb on + strand, within CCNA_00228 at 244.146 kb on + strand, within CCNA_00228 at 244.146 kb on + strand, within CCNA_00228 at 244.154 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.160 kb on + strand, within CCNA_00228 at 244.161 kb on + strand, within CCNA_00228 at 244.187 kb on - strand, within CCNA_00228 at 244.187 kb on - strand at 244.187 kb on - strand, within CCNA_00228 at 244.223 kb on + strand, within CCNA_00228 at 244.245 kb on + strand, within CCNA_00228 at 244.294 kb on + strand, within CCNA_00228 at 244.313 kb on + strand, within CCNA_00228 at 244.321 kb on - strand, within CCNA_00228 at 244.323 kb on + strand, within CCNA_00228 at 244.323 kb on + strand, within CCNA_00228 at 244.325 kb on - strand, within CCNA_00228 at 244.327 kb on + strand, within CCNA_00228 at 244.327 kb on + strand, within CCNA_00228 at 244.332 kb on + strand, within CCNA_00228 at 244.335 kb on - strand, within CCNA_00228 at 244.335 kb on - strand, within CCNA_00228 at 244.351 kb on + strand, within CCNA_00228 at 244.359 kb on - strand, within CCNA_00228 at 244.359 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.405 kb on - strand, within CCNA_00228 at 244.415 kb on - strand, within CCNA_00228 at 244.415 kb on - strand, within CCNA_00228 at 244.546 kb on - strand, within CCNA_00228 at 244.589 kb on - strand, within CCNA_00228 at 244.620 kb on + strand at 244.628 kb on - strand at 244.635 kb on + strand at 244.651 kb on + strand at 244.656 kb on + strand at 244.688 kb on - strand at 244.735 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.803 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.811 kb on - strand at 244.813 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.872 kb on + strand at 244.924 kb on + strand, within CCNA_00229 at 244.927 kb on - strand, within CCNA_00229 at 244.943 kb on + strand, within CCNA_00229 at 244.950 kb on + strand, within CCNA_00229 at 244.950 kb on + strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.975 kb on + strand, within CCNA_00229 at 245.002 kb on + strand, within CCNA_00229 at 245.054 kb on + strand, within CCNA_00229 at 245.056 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.079 kb on + strand, within CCNA_00229 at 245.093 kb on - strand, within CCNA_00229 at 245.096 kb on + strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.196 kb on + strand, within CCNA_00229 at 245.196 kb on + strand, within CCNA_00229 at 245.201 kb on + strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.206 kb on + strand, within CCNA_00229 at 245.209 kb on - strand, within CCNA_00229 at 245.209 kb on - strand, within CCNA_00229 at 245.214 kb on - strand, within CCNA_00229 at 245.215 kb on + strand, within CCNA_00229 at 245.257 kb on - strand, within CCNA_00229 at 245.270 kb on - strand, within CCNA_00229 at 245.285 kb on + strand, within CCNA_00229 at 245.297 kb on - strand, within CCNA_00229 at 245.297 kb on - strand, within CCNA_00229 at 245.307 kb on - strand, within CCNA_00229 at 245.402 kb on + strand, within CCNA_00229 at 245.402 kb on + strand, within CCNA_00229 at 245.424 kb on + strand, within CCNA_00229 at 245.441 kb on + strand, within CCNA_00229 at 245.449 kb on - strand, within CCNA_00229 at 245.476 kb on - strand, within CCNA_00229 at 245.486 kb on + strand, within CCNA_00229 at 245.494 kb on - strand, within CCNA_00229 at 245.508 kb on + strand at 245.508 kb on + strand, within CCNA_00229 at 245.513 kb on + strand, within CCNA_00229 at 245.518 kb on + strand, within CCNA_00229 at 245.518 kb on + strand, within CCNA_00229 at 245.519 kb on + strand, within CCNA_00229 at 245.521 kb on - strand, within CCNA_00229 at 245.526 kb on - strand, within CCNA_00229 at 245.537 kb on - strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.620 kb on + strand, within CCNA_00229 at 245.651 kb on - strand, within CCNA_00229 at 245.656 kb on - strand, within CCNA_00229 at 245.666 kb on + strand, within CCNA_00229 at 245.666 kb on + strand, within CCNA_00229 at 245.667 kb on + strand at 245.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 243,068 - -0.2 243,083 + -1.1 243,091 - -1.3 243,154 + -1.7 243,162 - -1.1 243,162 - -1.1 243,162 - +0.2 243,162 - +0.5 243,164 + -0.1 243,164 + +0.6 243,164 + +1.3 243,198 + CCNA_00226 0.15 -1.0 243,198 + CCNA_00226 0.15 -1.8 243,202 - CCNA_00226 0.16 +0.8 243,205 + CCNA_00226 0.17 +0.7 243,206 - CCNA_00226 0.18 -1.2 243,213 + CCNA_00226 0.20 -0.4 243,246 - CCNA_00226 0.32 -1.5 243,259 + CCNA_00226 0.37 +0.0 243,275 + CCNA_00226 0.43 -2.3 243,275 + CCNA_00226 0.43 +2.9 243,276 - CCNA_00226 0.43 -1.6 243,283 - CCNA_00226 0.46 -0.8 243,304 + CCNA_00226 0.53 -1.7 243,400 + CCNA_00226 0.88 +0.4 243,424 - +0.0 243,431 - +0.1 243,434 + +0.9 243,449 + +0.7 243,449 + +0.7 243,449 + +0.5 243,457 - +0.3 243,457 - +0.5 243,457 - -0.0 243,457 - +0.6 243,462 + +0.3 243,470 - +1.1 243,479 - +1.3 243,489 - +0.1 243,489 - -0.1 243,495 - +0.9 243,528 - -1.1 243,539 + -0.9 243,547 - CCNA_00227 0.10 -2.1 243,557 - CCNA_00227 0.12 +0.4 243,557 - CCNA_00227 0.12 -0.1 243,557 - CCNA_00227 0.12 +0.5 243,598 - CCNA_00227 0.19 -0.6 243,620 + CCNA_00227 0.23 -0.3 243,647 - CCNA_00227 0.28 -0.3 243,647 - CCNA_00227 0.28 +0.2 243,673 - CCNA_00227 0.32 -2.0 243,708 + CCNA_00227 0.38 +0.4 243,729 + CCNA_00227 0.42 +0.8 243,729 + CCNA_00227 0.42 -1.6 243,729 + CCNA_00227 0.42 -0.9 243,737 - CCNA_00227 0.44 +1.6 243,751 - CCNA_00227 0.46 -0.7 243,756 - CCNA_00227 0.47 -1.9 243,756 - CCNA_00227 0.47 -1.6 243,767 + CCNA_00227 0.49 -2.1 243,783 - CCNA_00227 0.52 -0.4 243,783 - CCNA_00227 0.52 +1.4 243,844 + CCNA_00227 0.62 +0.4 243,848 - CCNA_00227 0.63 -0.2 243,869 + CCNA_00227 0.67 -1.8 243,932 - CCNA_00227 0.78 -1.4 243,932 - CCNA_00227 0.78 +1.1 243,939 - CCNA_00227 0.79 -2.7 243,955 + CCNA_00227 0.82 +0.6 243,956 - CCNA_00227 0.82 +0.1 243,977 - CCNA_00227 0.86 +1.2 244,016 + -0.8 244,016 + -0.8 244,022 + +1.1 244,062 - -0.4 244,063 + +1.1 244,063 + -0.1 244,063 + -1.2 244,063 + +0.5 244,063 + +0.6 244,063 + -0.1 244,063 + +0.4 244,063 + -0.4 244,063 + +1.1 244,068 + -0.9 244,068 + -0.6 244,071 - +1.3 244,071 - -1.3 244,071 - +1.2 244,071 - -1.6 244,071 - +0.1 244,076 - +0.2 244,076 - -2.0 244,076 - +0.8 244,112 - +1.1 244,112 - -1.0 244,135 + CCNA_00228 0.13 -1.4 244,146 + CCNA_00228 0.15 +0.4 244,146 + CCNA_00228 0.15 +0.2 244,154 - CCNA_00228 0.16 -0.9 244,155 - CCNA_00228 0.16 -0.1 244,155 - CCNA_00228 0.16 -0.2 244,155 - CCNA_00228 0.16 +0.1 244,160 + CCNA_00228 0.17 -2.5 244,161 + CCNA_00228 0.17 -0.2 244,187 - CCNA_00228 0.21 -0.6 244,187 - +2.9 244,187 - CCNA_00228 0.21 -1.4 244,223 + CCNA_00228 0.27 +0.2 244,245 + CCNA_00228 0.31 -0.1 244,294 + CCNA_00228 0.39 -0.5 244,313 + CCNA_00228 0.42 -1.1 244,321 - CCNA_00228 0.43 -0.4 244,323 + CCNA_00228 0.43 -1.0 244,323 + CCNA_00228 0.43 -0.5 244,325 - CCNA_00228 0.44 +0.6 244,327 + CCNA_00228 0.44 -0.8 244,327 + CCNA_00228 0.44 -1.2 244,332 + CCNA_00228 0.45 +0.2 244,335 - CCNA_00228 0.45 +0.4 244,335 - CCNA_00228 0.45 -2.5 244,351 + CCNA_00228 0.48 +1.4 244,359 - CCNA_00228 0.49 -1.0 244,359 - CCNA_00228 0.49 +2.4 244,369 - CCNA_00228 0.51 -0.6 244,369 - CCNA_00228 0.51 -1.4 244,369 - CCNA_00228 0.51 +0.7 244,369 - CCNA_00228 0.51 -1.1 244,392 + CCNA_00228 0.55 +0.8 244,392 + CCNA_00228 0.55 +1.6 244,392 + CCNA_00228 0.55 -1.2 244,400 - CCNA_00228 0.56 +1.9 244,400 - CCNA_00228 0.56 +0.9 244,400 - CCNA_00228 0.56 -1.0 244,405 - CCNA_00228 0.57 +0.4 244,415 - CCNA_00228 0.58 -0.6 244,415 - CCNA_00228 0.58 -1.5 244,546 - CCNA_00228 0.80 -0.3 244,589 - CCNA_00228 0.87 -1.3 244,620 + -0.3 244,628 - -1.4 244,635 + +0.2 244,651 + +1.4 244,656 + +0.8 244,688 - -0.8 244,735 + -0.7 244,758 + +1.9 244,758 + +0.8 244,758 + +0.2 244,758 + -1.8 244,766 - -1.9 244,766 - +0.2 244,766 - +1.4 244,803 + -0.4 244,807 + +1.6 244,807 + -0.5 244,807 + -1.3 244,807 + +0.2 244,807 + +1.1 244,807 + +0.2 244,811 - +0.0 244,813 - -0.6 244,815 - -0.7 244,815 - +1.6 244,815 - +0.0 244,815 - +0.9 244,872 + +0.6 244,924 + CCNA_00229 0.14 -0.6 244,927 - CCNA_00229 0.14 -0.5 244,943 + CCNA_00229 0.16 +0.9 244,950 + CCNA_00229 0.16 -1.1 244,950 + CCNA_00229 0.16 -0.8 244,951 - CCNA_00229 0.17 -2.3 244,951 - CCNA_00229 0.17 -0.8 244,951 - CCNA_00229 0.17 +0.0 244,951 - CCNA_00229 0.17 -1.9 244,951 - CCNA_00229 0.17 -0.6 244,951 - CCNA_00229 0.17 -1.3 244,975 + CCNA_00229 0.19 +1.4 245,002 + CCNA_00229 0.22 -1.1 245,054 + CCNA_00229 0.27 +1.0 245,056 + CCNA_00229 0.27 -1.4 245,060 + CCNA_00229 0.28 -1.6 245,060 + CCNA_00229 0.28 +1.1 245,060 + CCNA_00229 0.28 +1.1 245,079 + CCNA_00229 0.30 -0.1 245,093 - CCNA_00229 0.31 -2.8 245,096 + CCNA_00229 0.31 +0.9 245,194 - CCNA_00229 0.41 -1.1 245,194 - CCNA_00229 0.41 -1.0 245,194 - CCNA_00229 0.41 +0.6 245,194 - CCNA_00229 0.41 -1.8 245,196 + CCNA_00229 0.42 +1.0 245,196 + CCNA_00229 0.42 -0.9 245,201 + CCNA_00229 0.42 +2.1 245,204 - CCNA_00229 0.42 -0.3 245,204 - CCNA_00229 0.42 -1.2 245,204 - CCNA_00229 0.42 -2.3 245,206 + CCNA_00229 0.43 -0.2 245,209 - CCNA_00229 0.43 +0.8 245,209 - CCNA_00229 0.43 +1.1 245,214 - CCNA_00229 0.43 -2.1 245,215 + CCNA_00229 0.44 -0.9 245,257 - CCNA_00229 0.48 -1.8 245,270 - CCNA_00229 0.49 +1.2 245,285 + CCNA_00229 0.51 +1.5 245,297 - CCNA_00229 0.52 +0.9 245,297 - CCNA_00229 0.52 -0.4 245,307 - CCNA_00229 0.53 -1.9 245,402 + CCNA_00229 0.63 +1.8 245,402 + CCNA_00229 0.63 +0.2 245,424 + CCNA_00229 0.65 +1.6 245,441 + CCNA_00229 0.67 -0.4 245,449 - CCNA_00229 0.68 -0.1 245,476 - CCNA_00229 0.70 -1.5 245,486 + CCNA_00229 0.71 +0.8 245,494 - CCNA_00229 0.72 -0.8 245,508 + +2.4 245,508 + CCNA_00229 0.74 -2.0 245,513 + CCNA_00229 0.74 -0.8 245,518 + CCNA_00229 0.75 -2.1 245,518 + CCNA_00229 0.75 -0.6 245,519 + CCNA_00229 0.75 -0.9 245,521 - CCNA_00229 0.75 +0.1 245,526 - CCNA_00229 0.75 +1.9 245,537 - CCNA_00229 0.77 -0.8 245,573 + CCNA_00229 0.80 +3.2 245,573 + CCNA_00229 0.80 -1.2 245,573 + CCNA_00229 0.80 -1.4 245,620 + CCNA_00229 0.85 +0.1 245,651 - CCNA_00229 0.88 -1.8 245,656 - CCNA_00229 0.89 -0.9 245,666 + CCNA_00229 0.90 -0.8 245,666 + CCNA_00229 0.90 -1.4 245,667 + +0.9 245,668 + -3.1
Or see this region's nucleotide sequence