Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00218

Experiment: PYE with nitrite 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00217 and CCNA_00218 are separated by 3 nucleotidesCCNA_00218 and CCNA_00220 are separated by 152 nucleotidesCCNA_00220 and CCNA_00221 are separated by 6 nucleotides CCNA_00217: CCNA_00217 - thiol:disulfide interchange protein DsbD, at 233,977 to 236,127 _00217 CCNA_00218: CCNA_00218 - hypothetical protein, at 236,131 to 236,877 _00218 CCNA_00220: CCNA_00220 - thiol-disulfide isomerase and thioredoxin, at 237,030 to 237,635 _00220 CCNA_00221: CCNA_00221 - hypothetical protein, at 237,642 to 238,136 _00221 Position (kb) 236 237Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 235.644 kb on + strand, within CCNA_00217at 235.658 kb on - strand, within CCNA_00217at 235.688 kb on - strand, within CCNA_00217at 236.175 kb on + strandat 236.175 kb on + strandat 236.186 kb on + strandat 236.228 kb on + strand, within CCNA_00218at 236.236 kb on - strand, within CCNA_00218at 236.255 kb on + strand, within CCNA_00218at 236.259 kb on + strand, within CCNA_00218at 236.263 kb on - strand, within CCNA_00218at 236.267 kb on + strand, within CCNA_00218at 236.277 kb on + strand, within CCNA_00218at 236.284 kb on + strand, within CCNA_00218at 236.301 kb on - strand, within CCNA_00218at 236.339 kb on + strand, within CCNA_00218at 236.345 kb on - strand, within CCNA_00218at 236.386 kb on + strand, within CCNA_00218at 236.386 kb on + strand, within CCNA_00218at 236.386 kb on + strand, within CCNA_00218at 236.387 kb on + strand, within CCNA_00218at 236.394 kb on - strand, within CCNA_00218at 236.394 kb on - strand, within CCNA_00218at 236.394 kb on - strand, within CCNA_00218at 236.394 kb on - strand, within CCNA_00218at 236.395 kb on - strand, within CCNA_00218at 236.412 kb on - strand, within CCNA_00218at 236.429 kb on + strand, within CCNA_00218at 236.429 kb on + strand, within CCNA_00218at 236.429 kb on + strand, within CCNA_00218at 236.437 kb on - strand, within CCNA_00218at 236.437 kb on - strand, within CCNA_00218at 236.445 kb on + strand, within CCNA_00218at 236.453 kb on + strand, within CCNA_00218at 236.453 kb on + strand, within CCNA_00218at 236.479 kb on + strand, within CCNA_00218at 236.479 kb on + strand, within CCNA_00218at 236.487 kb on - strand, within CCNA_00218at 236.519 kb on + strand, within CCNA_00218at 236.519 kb on + strand, within CCNA_00218at 236.519 kb on + strand, within CCNA_00218at 236.519 kb on + strand, within CCNA_00218at 236.519 kb on + strand, within CCNA_00218at 236.527 kb on - strand, within CCNA_00218at 236.563 kb on - strand, within CCNA_00218at 236.586 kb on - strand, within CCNA_00218at 236.591 kb on - strand, within CCNA_00218at 236.608 kb on + strand, within CCNA_00218at 236.671 kb on - strand, within CCNA_00218at 236.689 kb on - strand, within CCNA_00218at 236.689 kb on - strand, within CCNA_00218at 236.691 kb on + strand, within CCNA_00218at 236.691 kb on + strand, within CCNA_00218at 236.699 kb on - strand, within CCNA_00218at 236.702 kb on + strand, within CCNA_00218at 236.702 kb on + strand, within CCNA_00218at 236.710 kb on - strand, within CCNA_00218at 236.752 kb on - strand, within CCNA_00218at 236.786 kb on + strand, within CCNA_00218at 236.791 kb on + strand, within CCNA_00218at 236.799 kb on - strand, within CCNA_00218at 236.810 kb on + strandat 236.810 kb on + strandat 236.815 kb on + strandat 236.817 kb on + strandat 236.820 kb on - strandat 236.825 kb on - strandat 236.830 kb on + strandat 236.917 kb on + strandat 236.946 kb on + strandat 236.954 kb on - strandat 237.060 kb on + strandat 237.093 kb on + strand, within CCNA_00220at 237.188 kb on - strand, within CCNA_00220at 237.193 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.195 kb on + strand, within CCNA_00220at 237.203 kb on - strand, within CCNA_00220at 237.203 kb on - strand, within CCNA_00220at 237.205 kb on + strand, within CCNA_00220at 237.244 kb on + strand, within CCNA_00220at 237.244 kb on + strand, within CCNA_00220at 237.244 kb on - strand, within CCNA_00220at 237.248 kb on + strand, within CCNA_00220at 237.281 kb on + strand, within CCNA_00220at 237.344 kb on + strand, within CCNA_00220at 237.347 kb on - strand, within CCNA_00220at 237.365 kb on + strand, within CCNA_00220at 237.371 kb on + strand, within CCNA_00220at 237.371 kb on + strand, within CCNA_00220at 237.378 kb on + strand, within CCNA_00220at 237.379 kb on - strand, within CCNA_00220at 237.379 kb on - strand, within CCNA_00220at 237.406 kb on - strand, within CCNA_00220at 237.419 kb on - strand, within CCNA_00220at 237.422 kb on + strand, within CCNA_00220at 237.424 kb on - strand, within CCNA_00220at 237.424 kb on - strand, within CCNA_00220at 237.424 kb on - strand, within CCNA_00220at 237.430 kb on - strand, within CCNA_00220at 237.430 kb on - strand, within CCNA_00220at 237.430 kb on - strand, within CCNA_00220at 237.458 kb on + strand, within CCNA_00220at 237.466 kb on - strand, within CCNA_00220at 237.470 kb on + strand, within CCNA_00220at 237.518 kb on + strand, within CCNA_00220at 237.552 kb on - strand, within CCNA_00220at 237.559 kb on + strand, within CCNA_00220at 237.608 kb on - strandat 237.608 kb on - strandat 237.615 kb on + strandat 237.620 kb on + strandat 237.628 kb on - strandat 237.628 kb on - strandat 237.628 kb on - strandat 237.639 kb on + strandat 237.647 kb on - strandat 237.667 kb on + strandat 237.667 kb on - strandat 237.672 kb on + strandat 237.674 kb on + strandat 237.674 kb on + strandat 237.675 kb on - strandat 237.680 kb on - strandat 237.680 kb on - strandat 237.682 kb on - strandat 237.713 kb on + strand, within CCNA_00221at 237.713 kb on + strand, within CCNA_00221at 237.713 kb on + strand, within CCNA_00221at 237.713 kb on + strand, within CCNA_00221at 237.715 kb on + strand, within CCNA_00221at 237.715 kb on + strand, within CCNA_00221at 237.721 kb on - strand, within CCNA_00221at 237.739 kb on - strand, within CCNA_00221at 237.773 kb on + strand, within CCNA_00221at 237.773 kb on + strand, within CCNA_00221at 237.773 kb on + strand, within CCNA_00221at 237.774 kb on + strand, within CCNA_00221at 237.781 kb on - strand, within CCNA_00221at 237.794 kb on + strand, within CCNA_00221at 237.794 kb on + strand, within CCNA_00221at 237.799 kb on + strand, within CCNA_00221at 237.802 kb on - strand, within CCNA_00221at 237.815 kb on - strand, within CCNA_00221at 237.815 kb on - strand, within CCNA_00221

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM
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235,644 + CCNA_00217 0.77 -1.0
235,658 - CCNA_00217 0.78 +1.0
235,688 - CCNA_00217 0.80 -1.2
236,175 + +3.7
236,175 + +4.4
236,186 + -0.8
236,228 + CCNA_00218 0.13 -1.0
236,236 - CCNA_00218 0.14 +2.2
236,255 + CCNA_00218 0.17 -0.0
236,259 + CCNA_00218 0.17 +0.1
236,263 - CCNA_00218 0.18 -0.1
236,267 + CCNA_00218 0.18 +0.3
236,277 + CCNA_00218 0.20 -0.3
236,284 + CCNA_00218 0.20 -0.2
236,301 - CCNA_00218 0.23 +1.9
236,339 + CCNA_00218 0.28 +0.2
236,345 - CCNA_00218 0.29 +1.9
236,386 + CCNA_00218 0.34 +0.2
236,386 + CCNA_00218 0.34 +0.5
236,386 + CCNA_00218 0.34 +1.2
236,387 + CCNA_00218 0.34 +0.4
236,394 - CCNA_00218 0.35 -0.9
236,394 - CCNA_00218 0.35 -0.4
236,394 - CCNA_00218 0.35 +0.3
236,394 - CCNA_00218 0.35 -0.5
236,395 - CCNA_00218 0.35 +1.9
236,412 - CCNA_00218 0.38 +1.1
236,429 + CCNA_00218 0.40 -2.3
236,429 + CCNA_00218 0.40 +1.3
236,429 + CCNA_00218 0.40 -2.0
236,437 - CCNA_00218 0.41 +0.2
236,437 - CCNA_00218 0.41 +0.6
236,445 + CCNA_00218 0.42 -2.2
236,453 + CCNA_00218 0.43 +1.4
236,453 + CCNA_00218 0.43 -0.2
236,479 + CCNA_00218 0.47 -1.2
236,479 + CCNA_00218 0.47 +2.0
236,487 - CCNA_00218 0.48 +0.4
236,519 + CCNA_00218 0.52 -0.1
236,519 + CCNA_00218 0.52 -0.2
236,519 + CCNA_00218 0.52 -1.5
236,519 + CCNA_00218 0.52 -0.7
236,519 + CCNA_00218 0.52 -0.1
236,527 - CCNA_00218 0.53 -2.1
236,563 - CCNA_00218 0.58 +0.9
236,586 - CCNA_00218 0.61 +0.4
236,591 - CCNA_00218 0.62 +1.0
236,608 + CCNA_00218 0.64 -0.6
236,671 - CCNA_00218 0.72 +1.1
236,689 - CCNA_00218 0.75 +0.2
236,689 - CCNA_00218 0.75 +0.1
236,691 + CCNA_00218 0.75 -1.3
236,691 + CCNA_00218 0.75 -0.2
236,699 - CCNA_00218 0.76 +1.8
236,702 + CCNA_00218 0.76 -0.7
236,702 + CCNA_00218 0.76 -1.0
236,710 - CCNA_00218 0.78 +1.0
236,752 - CCNA_00218 0.83 +2.2
236,786 + CCNA_00218 0.88 +1.2
236,791 + CCNA_00218 0.88 +1.1
236,799 - CCNA_00218 0.89 +0.5
236,810 + -1.9
236,810 + -2.1
236,815 + +1.0
236,817 + -0.2
236,820 - +0.8
236,825 - -0.4
236,830 + +2.0
236,917 + -1.9
236,946 + +1.2
236,954 - -2.0
237,060 + +0.4
237,093 + CCNA_00220 0.10 -0.7
237,188 - CCNA_00220 0.26 +0.3
237,193 + CCNA_00220 0.27 +3.0
237,195 + CCNA_00220 0.27 +2.0
237,195 + CCNA_00220 0.27 +2.8
237,195 + CCNA_00220 0.27 +2.2
237,195 + CCNA_00220 0.27 +3.1
237,195 + CCNA_00220 0.27 +2.3
237,195 + CCNA_00220 0.27 +1.9
237,203 - CCNA_00220 0.29 +0.5
237,203 - CCNA_00220 0.29 -0.0
237,205 + CCNA_00220 0.29 +0.1
237,244 + CCNA_00220 0.35 -1.1
237,244 + CCNA_00220 0.35 -0.2
237,244 - CCNA_00220 0.35 -1.2
237,248 + CCNA_00220 0.36 +0.6
237,281 + CCNA_00220 0.41 +0.4
237,344 + CCNA_00220 0.52 -1.0
237,347 - CCNA_00220 0.52 -1.2
237,365 + CCNA_00220 0.55 +1.0
237,371 + CCNA_00220 0.56 -0.8
237,371 + CCNA_00220 0.56 +3.6
237,378 + CCNA_00220 0.57 +0.4
237,379 - CCNA_00220 0.58 -0.9
237,379 - CCNA_00220 0.58 -1.1
237,406 - CCNA_00220 0.62 +0.7
237,419 - CCNA_00220 0.64 -0.5
237,422 + CCNA_00220 0.65 -1.0
237,424 - CCNA_00220 0.65 +0.5
237,424 - CCNA_00220 0.65 +0.5
237,424 - CCNA_00220 0.65 -1.3
237,430 - CCNA_00220 0.66 +2.1
237,430 - CCNA_00220 0.66 -0.7
237,430 - CCNA_00220 0.66 -1.1
237,458 + CCNA_00220 0.71 -0.9
237,466 - CCNA_00220 0.72 -0.9
237,470 + CCNA_00220 0.73 +0.5
237,518 + CCNA_00220 0.81 +0.3
237,552 - CCNA_00220 0.86 -1.4
237,559 + CCNA_00220 0.87 +1.0
237,608 - -0.5
237,608 - -1.9
237,615 + +0.4
237,620 + -0.2
237,628 - -2.4
237,628 - +0.0
237,628 - +0.7
237,639 + -1.1
237,647 - -2.3
237,667 + -0.5
237,667 - +0.8
237,672 + -1.0
237,674 + -1.1
237,674 + +0.2
237,675 - +0.7
237,680 - -1.3
237,680 - -0.4
237,682 - +1.8
237,713 + CCNA_00221 0.14 +0.6
237,713 + CCNA_00221 0.14 +2.2
237,713 + CCNA_00221 0.14 -1.2
237,713 + CCNA_00221 0.14 -1.3
237,715 + CCNA_00221 0.15 -0.7
237,715 + CCNA_00221 0.15 +0.2
237,721 - CCNA_00221 0.16 -0.0
237,739 - CCNA_00221 0.20 +0.8
237,773 + CCNA_00221 0.26 -2.1
237,773 + CCNA_00221 0.26 +1.2
237,773 + CCNA_00221 0.26 -1.2
237,774 + CCNA_00221 0.27 -0.1
237,781 - CCNA_00221 0.28 -0.7
237,794 + CCNA_00221 0.31 +0.8
237,794 + CCNA_00221 0.31 +0.4
237,799 + CCNA_00221 0.32 -0.6
237,802 - CCNA_00221 0.32 -1.7
237,815 - CCNA_00221 0.35 -1.0
237,815 - CCNA_00221 0.35 +0.1

Or see this region's nucleotide sequence