Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00144 and CCNA_00145 overlap by 8 nucleotides CCNA_00145 and CCNA_00146 are separated by 24 nucleotides CCNA_00146 and CCNA_00147 are separated by 167 nucleotides CCNA_00147 and CCNA_00148 are separated by 75 nucleotides
CCNA_00144: CCNA_00144 - oxygen-independent coproporphyrinogen-III oxidase, at 155,819 to 156,973
_00144
CCNA_00145: CCNA_00145 - xanthosine triphosphate pyrophosphatase, at 156,966 to 157,568
_00145
CCNA_00146: CCNA_00146 - glutathione S-transferase, at 157,593 to 158,186
_00146
CCNA_00147: CCNA_00147 - hypothetical protein, at 158,354 to 158,470
_00147
CCNA_00148: CCNA_00148 - hypothetical protein, at 158,546 to 159,079
_00148
Position (kb) 156
157
158 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 155.968 kb on + strand, within CCNA_00144 at 155.968 kb on + strand, within CCNA_00144 at 155.968 kb on + strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.976 kb on - strand, within CCNA_00144 at 155.999 kb on + strand, within CCNA_00144 at 156.000 kb on + strand, within CCNA_00144 at 156.008 kb on - strand, within CCNA_00144 at 156.008 kb on - strand, within CCNA_00144 at 156.014 kb on + strand, within CCNA_00144 at 156.014 kb on + strand, within CCNA_00144 at 156.014 kb on + strand, within CCNA_00144 at 156.014 kb on + strand, within CCNA_00144 at 156.014 kb on + strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.022 kb on - strand, within CCNA_00144 at 156.089 kb on - strand, within CCNA_00144 at 156.092 kb on - strand, within CCNA_00144 at 156.097 kb on - strand, within CCNA_00144 at 156.154 kb on - strand, within CCNA_00144 at 156.206 kb on - strand, within CCNA_00144 at 156.211 kb on - strand, within CCNA_00144 at 156.227 kb on - strand, within CCNA_00144 at 156.227 kb on - strand, within CCNA_00144 at 156.244 kb on + strand, within CCNA_00144 at 156.244 kb on - strand, within CCNA_00144 at 156.252 kb on - strand, within CCNA_00144 at 156.288 kb on + strand, within CCNA_00144 at 156.288 kb on + strand, within CCNA_00144 at 156.296 kb on - strand, within CCNA_00144 at 156.324 kb on - strand, within CCNA_00144 at 156.326 kb on + strand, within CCNA_00144 at 156.334 kb on - strand, within CCNA_00144 at 156.360 kb on - strand, within CCNA_00144 at 156.361 kb on - strand, within CCNA_00144 at 156.361 kb on - strand, within CCNA_00144 at 156.385 kb on - strand, within CCNA_00144 at 156.390 kb on + strand, within CCNA_00144 at 156.390 kb on + strand, within CCNA_00144 at 156.398 kb on - strand, within CCNA_00144 at 156.408 kb on - strand, within CCNA_00144 at 156.415 kb on - strand, within CCNA_00144 at 156.433 kb on - strand, within CCNA_00144 at 156.452 kb on + strand, within CCNA_00144 at 156.452 kb on + strand, within CCNA_00144 at 156.460 kb on - strand, within CCNA_00144 at 156.460 kb on - strand, within CCNA_00144 at 156.468 kb on + strand, within CCNA_00144 at 156.468 kb on + strand, within CCNA_00144 at 156.468 kb on + strand, within CCNA_00144 at 156.476 kb on - strand, within CCNA_00144 at 156.481 kb on - strand, within CCNA_00144 at 156.540 kb on + strand, within CCNA_00144 at 156.554 kb on + strand, within CCNA_00144 at 156.556 kb on - strand, within CCNA_00144 at 156.602 kb on - strand, within CCNA_00144 at 156.605 kb on + strand, within CCNA_00144 at 156.605 kb on + strand, within CCNA_00144 at 156.623 kb on + strand, within CCNA_00144 at 156.645 kb on + strand, within CCNA_00144 at 156.645 kb on + strand, within CCNA_00144 at 156.677 kb on + strand, within CCNA_00144 at 156.688 kb on - strand, within CCNA_00144 at 156.699 kb on + strand, within CCNA_00144 at 156.802 kb on - strand, within CCNA_00144 at 156.816 kb on - strand, within CCNA_00144 at 156.819 kb on + strand, within CCNA_00144 at 156.819 kb on + strand, within CCNA_00144 at 156.819 kb on + strand, within CCNA_00144 at 156.820 kb on - strand, within CCNA_00144 at 156.827 kb on - strand, within CCNA_00144 at 156.827 kb on - strand, within CCNA_00144 at 156.845 kb on + strand, within CCNA_00144 at 156.888 kb on + strand at 156.888 kb on + strand at 156.888 kb on + strand at 156.896 kb on - strand at 156.896 kb on - strand at 156.921 kb on + strand at 156.921 kb on + strand at 156.921 kb on + strand at 156.921 kb on + strand at 156.921 kb on + strand at 156.929 kb on - strand at 156.929 kb on - strand at 156.929 kb on - strand at 156.965 kb on - strand at 156.982 kb on + strand at 157.053 kb on + strand, within CCNA_00145 at 157.053 kb on + strand, within CCNA_00145 at 157.060 kb on + strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.061 kb on - strand, within CCNA_00145 at 157.062 kb on - strand, within CCNA_00145 at 157.062 kb on - strand, within CCNA_00145 at 157.080 kb on + strand, within CCNA_00145 at 157.080 kb on + strand, within CCNA_00145 at 157.093 kb on - strand, within CCNA_00145 at 157.122 kb on + strand, within CCNA_00145 at 157.130 kb on - strand, within CCNA_00145 at 157.151 kb on - strand, within CCNA_00145 at 157.151 kb on - strand, within CCNA_00145 at 157.152 kb on - strand, within CCNA_00145 at 157.157 kb on - strand, within CCNA_00145 at 157.176 kb on + strand, within CCNA_00145 at 157.176 kb on + strand, within CCNA_00145 at 157.176 kb on + strand, within CCNA_00145 at 157.184 kb on - strand, within CCNA_00145 at 157.184 kb on - strand, within CCNA_00145 at 157.187 kb on + strand, within CCNA_00145 at 157.187 kb on + strand, within CCNA_00145 at 157.197 kb on + strand, within CCNA_00145 at 157.197 kb on + strand, within CCNA_00145 at 157.233 kb on - strand, within CCNA_00145 at 157.237 kb on - strand, within CCNA_00145 at 157.237 kb on - strand, within CCNA_00145 at 157.239 kb on + strand, within CCNA_00145 at 157.239 kb on + strand, within CCNA_00145 at 157.248 kb on - strand, within CCNA_00145 at 157.269 kb on + strand, within CCNA_00145 at 157.277 kb on - strand, within CCNA_00145 at 157.277 kb on - strand, within CCNA_00145 at 157.284 kb on + strand, within CCNA_00145 at 157.288 kb on - strand, within CCNA_00145 at 157.325 kb on - strand, within CCNA_00145 at 157.325 kb on - strand, within CCNA_00145 at 157.345 kb on - strand, within CCNA_00145 at 157.345 kb on - strand, within CCNA_00145 at 157.364 kb on + strand, within CCNA_00145 at 157.364 kb on + strand, within CCNA_00145 at 157.373 kb on + strand, within CCNA_00145 at 157.374 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.381 kb on - strand, within CCNA_00145 at 157.390 kb on + strand, within CCNA_00145 at 157.390 kb on + strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.398 kb on - strand, within CCNA_00145 at 157.434 kb on - strand, within CCNA_00145 at 157.445 kb on + strand, within CCNA_00145 at 157.500 kb on + strand, within CCNA_00145 at 157.516 kb on + strand at 157.517 kb on + strand at 157.538 kb on + strand at 157.550 kb on + strand at 157.550 kb on + strand at 157.681 kb on - strand, within CCNA_00146 at 157.695 kb on - strand, within CCNA_00146 at 157.705 kb on - strand, within CCNA_00146 at 157.741 kb on - strand, within CCNA_00146 at 157.748 kb on - strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.773 kb on + strand, within CCNA_00146 at 157.775 kb on + strand, within CCNA_00146 at 157.781 kb on - strand, within CCNA_00146 at 157.814 kb on + strand, within CCNA_00146 at 157.814 kb on + strand, within CCNA_00146 at 157.860 kb on + strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.868 kb on - strand, within CCNA_00146 at 157.887 kb on + strand, within CCNA_00146 at 157.902 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.948 kb on + strand, within CCNA_00146 at 157.956 kb on - strand, within CCNA_00146 at 157.956 kb on - strand, within CCNA_00146 at 157.965 kb on + strand, within CCNA_00146 at 157.978 kb on - strand, within CCNA_00146 at 158.016 kb on - strand, within CCNA_00146 at 158.071 kb on + strand, within CCNA_00146 at 158.079 kb on - strand, within CCNA_00146 at 158.124 kb on + strand, within CCNA_00146 at 158.128 kb on + strand at 158.139 kb on + strand at 158.147 kb on - strand at 158.149 kb on - strand at 158.172 kb on - strand at 158.174 kb on + strand at 158.181 kb on + strand at 158.181 kb on + strand at 158.189 kb on - strand at 158.194 kb on - strand at 158.291 kb on + strand at 158.294 kb on - strand at 158.299 kb on - strand at 158.299 kb on - strand at 158.309 kb on - strand at 158.334 kb on + strand at 158.366 kb on + strand, within CCNA_00147 at 158.434 kb on + strand, within CCNA_00147 at 158.463 kb on + strand at 158.463 kb on + strand at 158.464 kb on - strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.465 kb on + strand at 158.471 kb on - strand at 158.471 kb on - strand at 158.492 kb on - strand at 158.492 kb on - strand at 158.517 kb on + strand at 158.532 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand at 158.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 155,968 + CCNA_00144 0.13 -1.4 155,968 + CCNA_00144 0.13 -1.0 155,968 + CCNA_00144 0.13 -0.0 155,976 - CCNA_00144 0.14 -1.1 155,976 - CCNA_00144 0.14 -0.3 155,976 - CCNA_00144 0.14 -1.4 155,976 - CCNA_00144 0.14 +1.0 155,976 - CCNA_00144 0.14 -0.7 155,976 - CCNA_00144 0.14 +1.8 155,999 + CCNA_00144 0.16 -0.6 156,000 + CCNA_00144 0.16 -1.9 156,008 - CCNA_00144 0.16 +0.6 156,008 - CCNA_00144 0.16 +0.7 156,014 + CCNA_00144 0.17 -0.7 156,014 + CCNA_00144 0.17 +0.0 156,014 + CCNA_00144 0.17 +0.5 156,014 + CCNA_00144 0.17 -1.8 156,014 + CCNA_00144 0.17 +0.7 156,022 - CCNA_00144 0.18 +3.2 156,022 - CCNA_00144 0.18 +1.8 156,022 - CCNA_00144 0.18 -0.4 156,022 - CCNA_00144 0.18 +0.3 156,022 - CCNA_00144 0.18 +1.0 156,022 - CCNA_00144 0.18 +1.2 156,089 - CCNA_00144 0.23 -0.2 156,092 - CCNA_00144 0.24 -1.1 156,097 - CCNA_00144 0.24 +1.1 156,154 - CCNA_00144 0.29 -0.3 156,206 - CCNA_00144 0.34 -0.7 156,211 - CCNA_00144 0.34 +0.5 156,227 - CCNA_00144 0.35 -0.4 156,227 - CCNA_00144 0.35 -0.5 156,244 + CCNA_00144 0.37 -1.0 156,244 - CCNA_00144 0.37 +0.7 156,252 - CCNA_00144 0.37 +0.7 156,288 + CCNA_00144 0.41 +2.0 156,288 + CCNA_00144 0.41 -0.3 156,296 - CCNA_00144 0.41 +1.3 156,324 - CCNA_00144 0.44 +1.2 156,326 + CCNA_00144 0.44 +0.6 156,334 - CCNA_00144 0.45 +0.4 156,360 - CCNA_00144 0.47 -0.1 156,361 - CCNA_00144 0.47 +0.4 156,361 - CCNA_00144 0.47 -0.5 156,385 - CCNA_00144 0.49 -0.3 156,390 + CCNA_00144 0.49 -2.3 156,390 + CCNA_00144 0.49 +1.3 156,398 - CCNA_00144 0.50 +3.4 156,408 - CCNA_00144 0.51 +2.2 156,415 - CCNA_00144 0.52 +0.1 156,433 - CCNA_00144 0.53 -0.3 156,452 + CCNA_00144 0.55 -1.1 156,452 + CCNA_00144 0.55 -0.8 156,460 - CCNA_00144 0.55 -0.6 156,460 - CCNA_00144 0.55 +0.6 156,468 + CCNA_00144 0.56 +0.2 156,468 + CCNA_00144 0.56 +0.4 156,468 + CCNA_00144 0.56 -0.0 156,476 - CCNA_00144 0.57 +0.2 156,481 - CCNA_00144 0.57 +1.0 156,540 + CCNA_00144 0.62 -0.2 156,554 + CCNA_00144 0.64 -0.2 156,556 - CCNA_00144 0.64 -0.7 156,602 - CCNA_00144 0.68 +1.0 156,605 + CCNA_00144 0.68 +1.3 156,605 + CCNA_00144 0.68 -1.4 156,623 + CCNA_00144 0.70 +1.1 156,645 + CCNA_00144 0.72 -1.2 156,645 + CCNA_00144 0.72 +1.5 156,677 + CCNA_00144 0.74 -0.4 156,688 - CCNA_00144 0.75 -1.3 156,699 + CCNA_00144 0.76 -0.4 156,802 - CCNA_00144 0.85 +0.8 156,816 - CCNA_00144 0.86 -0.8 156,819 + CCNA_00144 0.87 +0.7 156,819 + CCNA_00144 0.87 +0.2 156,819 + CCNA_00144 0.87 -0.0 156,820 - CCNA_00144 0.87 -0.1 156,827 - CCNA_00144 0.87 -2.2 156,827 - CCNA_00144 0.87 +1.5 156,845 + CCNA_00144 0.89 -0.1 156,888 + +0.4 156,888 + -0.8 156,888 + +0.2 156,896 - +2.7 156,896 - +3.5 156,921 + +0.1 156,921 + -0.2 156,921 + +1.6 156,921 + -0.8 156,921 + -1.9 156,929 - -0.4 156,929 - +0.4 156,929 - -0.6 156,965 - -1.2 156,982 + +1.9 157,053 + CCNA_00145 0.14 +0.5 157,053 + CCNA_00145 0.14 -0.4 157,060 + CCNA_00145 0.16 -0.8 157,061 - CCNA_00145 0.16 +0.0 157,061 - CCNA_00145 0.16 +0.5 157,061 - CCNA_00145 0.16 +2.2 157,061 - CCNA_00145 0.16 +0.9 157,061 - CCNA_00145 0.16 +0.1 157,061 - CCNA_00145 0.16 -0.5 157,062 - CCNA_00145 0.16 -0.1 157,062 - CCNA_00145 0.16 -0.4 157,080 + CCNA_00145 0.19 +0.7 157,080 + CCNA_00145 0.19 +1.0 157,093 - CCNA_00145 0.21 +1.6 157,122 + CCNA_00145 0.26 +0.5 157,130 - CCNA_00145 0.27 -0.3 157,151 - CCNA_00145 0.31 +0.7 157,151 - CCNA_00145 0.31 -1.1 157,152 - CCNA_00145 0.31 -1.5 157,157 - CCNA_00145 0.32 +1.7 157,176 + CCNA_00145 0.35 -1.2 157,176 + CCNA_00145 0.35 +0.2 157,176 + CCNA_00145 0.35 +1.1 157,184 - CCNA_00145 0.36 +1.3 157,184 - CCNA_00145 0.36 +1.7 157,187 + CCNA_00145 0.37 +0.7 157,187 + CCNA_00145 0.37 +0.3 157,197 + CCNA_00145 0.38 -1.2 157,197 + CCNA_00145 0.38 +0.6 157,233 - CCNA_00145 0.44 +1.5 157,237 - CCNA_00145 0.45 +0.1 157,237 - CCNA_00145 0.45 +0.3 157,239 + CCNA_00145 0.45 +1.2 157,239 + CCNA_00145 0.45 +0.6 157,248 - CCNA_00145 0.47 +1.8 157,269 + CCNA_00145 0.50 +0.8 157,277 - CCNA_00145 0.52 +0.7 157,277 - CCNA_00145 0.52 +0.3 157,284 + CCNA_00145 0.53 +1.6 157,288 - CCNA_00145 0.53 +0.5 157,325 - CCNA_00145 0.60 +0.1 157,325 - CCNA_00145 0.60 +0.7 157,345 - CCNA_00145 0.63 -1.0 157,345 - CCNA_00145 0.63 +0.6 157,364 + CCNA_00145 0.66 +2.0 157,364 + CCNA_00145 0.66 -0.7 157,373 + CCNA_00145 0.67 -1.2 157,374 - CCNA_00145 0.68 +1.8 157,381 - CCNA_00145 0.69 -0.5 157,381 - CCNA_00145 0.69 +0.6 157,381 - CCNA_00145 0.69 +0.5 157,390 + CCNA_00145 0.70 -1.7 157,390 + CCNA_00145 0.70 -0.7 157,398 - CCNA_00145 0.72 +1.4 157,398 - CCNA_00145 0.72 +0.3 157,398 - CCNA_00145 0.72 +0.4 157,398 - CCNA_00145 0.72 +0.2 157,398 - CCNA_00145 0.72 +1.3 157,398 - CCNA_00145 0.72 +0.2 157,434 - CCNA_00145 0.78 +1.0 157,445 + CCNA_00145 0.79 -0.4 157,500 + CCNA_00145 0.89 -0.8 157,516 + +3.4 157,517 + +1.9 157,538 + -1.6 157,550 + -1.2 157,550 + +1.6 157,681 - CCNA_00146 0.15 +0.8 157,695 - CCNA_00146 0.17 +0.5 157,705 - CCNA_00146 0.19 +0.6 157,741 - CCNA_00146 0.25 -1.2 157,748 - CCNA_00146 0.26 -1.0 157,773 + CCNA_00146 0.30 -0.8 157,773 + CCNA_00146 0.30 +0.1 157,773 + CCNA_00146 0.30 -0.6 157,775 + CCNA_00146 0.31 -0.3 157,781 - CCNA_00146 0.32 -1.1 157,814 + CCNA_00146 0.37 -0.9 157,814 + CCNA_00146 0.37 +0.3 157,860 + CCNA_00146 0.45 -1.3 157,868 - CCNA_00146 0.46 -0.8 157,868 - CCNA_00146 0.46 +0.8 157,868 - CCNA_00146 0.46 +1.2 157,868 - CCNA_00146 0.46 -0.6 157,868 - CCNA_00146 0.46 -0.7 157,887 + CCNA_00146 0.49 +0.8 157,902 + CCNA_00146 0.52 -0.8 157,948 + CCNA_00146 0.60 +1.6 157,948 + CCNA_00146 0.60 +1.5 157,948 + CCNA_00146 0.60 -0.3 157,956 - CCNA_00146 0.61 -0.2 157,956 - CCNA_00146 0.61 -1.7 157,965 + CCNA_00146 0.63 +0.9 157,978 - CCNA_00146 0.65 -0.3 158,016 - CCNA_00146 0.71 +0.1 158,071 + CCNA_00146 0.80 +1.5 158,079 - CCNA_00146 0.82 +0.6 158,124 + CCNA_00146 0.89 +3.2 158,128 + -0.4 158,139 + -0.7 158,147 - -0.6 158,149 - -1.9 158,172 - -1.2 158,174 + -0.9 158,181 + -0.2 158,181 + -0.6 158,189 - -0.4 158,194 - +1.6 158,291 + +3.0 158,294 - +2.0 158,299 - +1.0 158,299 - +1.2 158,309 - -1.8 158,334 + +1.9 158,366 + CCNA_00147 0.10 +2.1 158,434 + CCNA_00147 0.68 +1.6 158,463 + +1.8 158,463 + +2.1 158,464 - -0.4 158,465 + +1.4 158,465 + +1.7 158,465 + +1.9 158,465 + +1.1 158,471 - -1.3 158,471 - -0.1 158,492 - -0.9 158,492 - +2.4 158,517 + +1.8 158,532 + +3.0 158,553 + +1.4 158,553 + +1.2 158,553 + +1.7
Or see this region's nucleotide sequence