Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00029

Experiment: PYE with nitrite 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00028 and CCNA_00029 are separated by 255 nucleotidesCCNA_00029 and CCNA_00030 are separated by 48 nucleotidesCCNA_00030 and CCNA_00031 overlap by 18 nucleotides CCNA_00028: CCNA_00028 - TonB-dependent receptor, at 30,337 to 32,658 _00028 CCNA_00029: CCNA_00029 - lysine exporter protein, at 32,914 to 33,546 _00029 CCNA_00030: CCNA_00030 - ribonuclease T2, at 33,595 to 34,329 _00030 CCNA_00031: CCNA_00031 - toxin protein VapC, at 34,312 to 34,698 _00031 Position (kb) 32 33 34Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 32.182 kb on - strand, within CCNA_00028at 32.656 kb on - strandat 32.808 kb on - strandat 32.841 kb on + strandat 32.936 kb on - strandat 32.945 kb on + strandat 32.947 kb on - strandat 32.947 kb on - strandat 32.965 kb on + strandat 32.966 kb on - strandat 32.972 kb on + strandat 32.991 kb on + strand, within CCNA_00029at 32.991 kb on - strand, within CCNA_00029at 33.022 kb on - strand, within CCNA_00029at 33.026 kb on - strand, within CCNA_00029at 33.027 kb on + strand, within CCNA_00029at 33.036 kb on - strand, within CCNA_00029at 33.045 kb on + strand, within CCNA_00029at 33.083 kb on + strand, within CCNA_00029at 33.088 kb on + strand, within CCNA_00029at 33.093 kb on + strand, within CCNA_00029at 33.096 kb on - strand, within CCNA_00029at 33.108 kb on + strand, within CCNA_00029at 33.116 kb on - strand, within CCNA_00029at 33.116 kb on - strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.138 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.236 kb on - strand, within CCNA_00029at 33.237 kb on - strand, within CCNA_00029at 33.237 kb on - strand, within CCNA_00029at 33.260 kb on - strand, within CCNA_00029at 33.279 kb on + strand, within CCNA_00029at 33.292 kb on - strand, within CCNA_00029at 33.298 kb on + strand, within CCNA_00029at 33.298 kb on + strand, within CCNA_00029at 33.335 kb on - strandat 33.358 kb on - strand, within CCNA_00029at 33.367 kb on + strand, within CCNA_00029at 33.370 kb on - strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.382 kb on - strand, within CCNA_00029at 33.384 kb on + strand, within CCNA_00029at 33.403 kb on - strand, within CCNA_00029at 33.456 kb on + strand, within CCNA_00029at 33.472 kb on - strand, within CCNA_00029at 33.499 kb on + strandat 33.499 kb on + strandat 33.499 kb on - strandat 33.499 kb on - strandat 33.562 kb on - strandat 33.565 kb on + strandat 33.671 kb on + strand, within CCNA_00030at 33.677 kb on - strand, within CCNA_00030at 33.679 kb on - strand, within CCNA_00030at 33.684 kb on + strand, within CCNA_00030at 33.684 kb on + strand, within CCNA_00030at 33.687 kb on - strand, within CCNA_00030at 33.687 kb on - strand, within CCNA_00030at 33.692 kb on - strand, within CCNA_00030at 33.692 kb on - strand, within CCNA_00030at 33.704 kb on - strand, within CCNA_00030at 33.749 kb on + strand, within CCNA_00030at 33.757 kb on - strand, within CCNA_00030at 33.757 kb on - strand, within CCNA_00030at 33.777 kb on - strand, within CCNA_00030at 33.781 kb on + strand, within CCNA_00030at 33.789 kb on - strand, within CCNA_00030at 33.789 kb on - strand, within CCNA_00030at 33.794 kb on - strand, within CCNA_00030at 33.796 kb on + strand, within CCNA_00030at 33.796 kb on + strand, within CCNA_00030at 33.804 kb on - strand, within CCNA_00030at 33.825 kb on - strand, within CCNA_00030at 33.856 kb on + strand, within CCNA_00030at 33.871 kb on + strand, within CCNA_00030at 33.871 kb on + strand, within CCNA_00030at 33.871 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.879 kb on - strand, within CCNA_00030at 33.917 kb on + strand, within CCNA_00030at 33.942 kb on + strand, within CCNA_00030at 33.950 kb on - strand, within CCNA_00030at 33.974 kb on - strand, within CCNA_00030at 33.974 kb on - strand, within CCNA_00030at 33.999 kb on + strand, within CCNA_00030at 34.007 kb on - strand, within CCNA_00030at 34.012 kb on + strand, within CCNA_00030at 34.042 kb on - strand, within CCNA_00030at 34.068 kb on - strand, within CCNA_00030at 34.135 kb on + strand, within CCNA_00030at 34.135 kb on + strand, within CCNA_00030at 34.155 kb on + strand, within CCNA_00030at 34.172 kb on + strand, within CCNA_00030at 34.176 kb on + strand, within CCNA_00030at 34.177 kb on + strand, within CCNA_00030at 34.180 kb on - strand, within CCNA_00030at 34.180 kb on - strand, within CCNA_00030at 34.184 kb on - strand, within CCNA_00030at 34.191 kb on + strand, within CCNA_00030at 34.199 kb on - strand, within CCNA_00030at 34.203 kb on - strand, within CCNA_00030at 34.214 kb on + strand, within CCNA_00030at 34.224 kb on - strand, within CCNA_00030at 34.226 kb on + strand, within CCNA_00030at 34.234 kb on - strand, within CCNA_00030at 34.239 kb on - strand, within CCNA_00030at 34.278 kb on - strandat 34.316 kb on - strandat 34.387 kb on + strand, within CCNA_00031at 34.388 kb on + strand, within CCNA_00031at 34.403 kb on - strand, within CCNA_00031at 34.414 kb on + strand, within CCNA_00031at 34.434 kb on - strand, within CCNA_00031at 34.455 kb on - strand, within CCNA_00031at 34.526 kb on - strand, within CCNA_00031at 34.526 kb on - strandat 34.544 kb on + strand, within CCNA_00031

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM
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32,182 - CCNA_00028 0.79 +3.4
32,656 - +0.9
32,808 - -1.0
32,841 + +1.9
32,936 - +1.2
32,945 + +1.9
32,947 - -0.2
32,947 - -0.1
32,965 + -0.9
32,966 - +2.1
32,972 + +0.8
32,991 + CCNA_00029 0.12 -1.5
32,991 - CCNA_00029 0.12 +2.4
33,022 - CCNA_00029 0.17 +0.4
33,026 - CCNA_00029 0.18 -2.3
33,027 + CCNA_00029 0.18 +1.3
33,036 - CCNA_00029 0.19 +0.1
33,045 + CCNA_00029 0.21 +1.6
33,083 + CCNA_00029 0.27 +1.6
33,088 + CCNA_00029 0.27 +0.5
33,093 + CCNA_00029 0.28 +0.4
33,096 - CCNA_00029 0.29 -1.1
33,108 + CCNA_00029 0.31 +0.2
33,116 - CCNA_00029 0.32 +0.3
33,116 - CCNA_00029 0.32 +0.5
33,128 + CCNA_00029 0.34 -0.2
33,128 + CCNA_00029 0.34 -0.9
33,128 + CCNA_00029 0.34 -0.5
33,128 + CCNA_00029 0.34 +0.6
33,136 - CCNA_00029 0.35 +2.6
33,136 - CCNA_00029 0.35 -1.1
33,136 - CCNA_00029 0.35 +1.0
33,136 - CCNA_00029 0.35 -1.1
33,136 - CCNA_00029 0.35 -1.6
33,138 + CCNA_00029 0.35 -1.1
33,228 + CCNA_00029 0.50 -0.7
33,228 + CCNA_00029 0.50 +1.8
33,228 + CCNA_00029 0.50 +1.5
33,228 + CCNA_00029 0.50 +0.0
33,236 - CCNA_00029 0.51 +0.2
33,237 - CCNA_00029 0.51 +0.2
33,237 - CCNA_00029 0.51 +0.8
33,260 - CCNA_00029 0.55 +0.8
33,279 + CCNA_00029 0.58 +2.0
33,292 - CCNA_00029 0.60 +0.3
33,298 + CCNA_00029 0.61 +0.6
33,298 + CCNA_00029 0.61 +0.5
33,335 - +1.2
33,358 - CCNA_00029 0.70 -0.9
33,367 + CCNA_00029 0.72 +0.7
33,370 - CCNA_00029 0.72 +0.8
33,379 + CCNA_00029 0.73 +0.3
33,379 + CCNA_00029 0.73 +1.7
33,382 - CCNA_00029 0.74 -1.3
33,384 + CCNA_00029 0.74 +2.0
33,403 - CCNA_00029 0.77 +0.9
33,456 + CCNA_00029 0.86 +1.9
33,472 - CCNA_00029 0.88 +1.5
33,499 + -1.5
33,499 + -0.5
33,499 - +1.4
33,499 - +0.1
33,562 - -0.9
33,565 + +3.6
33,671 + CCNA_00030 0.10 -0.6
33,677 - CCNA_00030 0.11 -0.2
33,679 - CCNA_00030 0.11 +1.2
33,684 + CCNA_00030 0.12 +0.6
33,684 + CCNA_00030 0.12 +0.2
33,687 - CCNA_00030 0.13 +0.3
33,687 - CCNA_00030 0.13 -1.6
33,692 - CCNA_00030 0.13 +0.9
33,692 - CCNA_00030 0.13 -0.5
33,704 - CCNA_00030 0.15 +0.6
33,749 + CCNA_00030 0.21 +1.5
33,757 - CCNA_00030 0.22 -1.2
33,757 - CCNA_00030 0.22 -0.8
33,777 - CCNA_00030 0.25 -0.0
33,781 + CCNA_00030 0.25 +0.3
33,789 - CCNA_00030 0.26 -0.6
33,789 - CCNA_00030 0.26 -0.7
33,794 - CCNA_00030 0.27 -0.2
33,796 + CCNA_00030 0.27 +1.4
33,796 + CCNA_00030 0.27 +0.1
33,804 - CCNA_00030 0.28 +1.4
33,825 - CCNA_00030 0.31 +0.5
33,856 + CCNA_00030 0.36 -1.1
33,871 + CCNA_00030 0.38 -0.8
33,871 + CCNA_00030 0.38 +0.6
33,871 - CCNA_00030 0.38 +0.1
33,879 - CCNA_00030 0.39 -0.1
33,879 - CCNA_00030 0.39 -0.0
33,879 - CCNA_00030 0.39 +0.8
33,917 + CCNA_00030 0.44 -1.5
33,942 + CCNA_00030 0.47 -0.0
33,950 - CCNA_00030 0.48 -0.3
33,974 - CCNA_00030 0.52 +0.4
33,974 - CCNA_00030 0.52 -0.8
33,999 + CCNA_00030 0.55 +0.5
34,007 - CCNA_00030 0.56 +0.2
34,012 + CCNA_00030 0.57 -0.3
34,042 - CCNA_00030 0.61 -0.4
34,068 - CCNA_00030 0.64 -1.0
34,135 + CCNA_00030 0.73 -0.8
34,135 + CCNA_00030 0.73 -0.9
34,155 + CCNA_00030 0.76 +0.4
34,172 + CCNA_00030 0.79 -0.9
34,176 + CCNA_00030 0.79 -1.9
34,177 + CCNA_00030 0.79 -0.7
34,180 - CCNA_00030 0.80 -1.7
34,180 - CCNA_00030 0.80 -1.2
34,184 - CCNA_00030 0.80 +0.4
34,191 + CCNA_00030 0.81 +0.1
34,199 - CCNA_00030 0.82 +0.2
34,203 - CCNA_00030 0.83 -0.4
34,214 + CCNA_00030 0.84 +4.4
34,224 - CCNA_00030 0.86 -2.0
34,226 + CCNA_00030 0.86 +1.2
34,234 - CCNA_00030 0.87 +1.3
34,239 - CCNA_00030 0.88 +2.6
34,278 - -0.4
34,316 - +1.1
34,387 + CCNA_00031 0.19 +0.1
34,388 + CCNA_00031 0.20 +0.3
34,403 - CCNA_00031 0.24 +2.1
34,414 + CCNA_00031 0.26 +1.6
34,434 - CCNA_00031 0.32 +3.3
34,455 - CCNA_00031 0.37 +3.0
34,526 - CCNA_00031 0.55 +4.5
34,526 - +0.8
34,544 + CCNA_00031 0.60 -0.6

Or see this region's nucleotide sequence