Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00028

Experiment: PYE with nitrite 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00026 and CCNA_00027 are separated by 98 nucleotidesCCNA_00027 and CCNA_00028 are separated by 145 nucleotidesCCNA_00028 and CCNA_00029 are separated by 255 nucleotidesCCNA_00029 and CCNA_00030 are separated by 48 nucleotides CCNA_00026: CCNA_00026 - PAS-family sensor histidine kinase, at 26,701 to 29,403 _00026 CCNA_00027: CCNA_00027 - 2OG-Fe(II) oxygenase, at 29,502 to 30,191 _00027 CCNA_00028: CCNA_00028 - TonB-dependent receptor, at 30,337 to 32,658 _00028 CCNA_00029: CCNA_00029 - lysine exporter protein, at 32,914 to 33,546 _00029 CCNA_00030: CCNA_00030 - ribonuclease T2, at 33,595 to 34,329 _00030 Position (kb) 30 31 32 33Strain fitness (log2 ratio) -2 -1 0 1 2 3at 29.345 kb on + strandat 29.345 kb on + strandat 29.345 kb on + strandat 29.353 kb on - strandat 29.449 kb on - strandat 29.471 kb on - strandat 29.508 kb on - strandat 29.586 kb on - strand, within CCNA_00027at 29.833 kb on + strand, within CCNA_00027at 29.841 kb on - strand, within CCNA_00027at 30.037 kb on - strand, within CCNA_00027at 30.189 kb on - strandat 30.251 kb on + strandat 30.284 kb on + strandat 30.292 kb on - strandat 30.294 kb on + strandat 30.302 kb on - strandat 30.312 kb on + strandat 30.320 kb on - strandat 30.322 kb on + strandat 30.322 kb on + strandat 30.322 kb on + strandat 30.330 kb on - strandat 30.330 kb on - strandat 30.330 kb on - strandat 30.330 kb on - strandat 30.330 kb on - strandat 30.335 kb on + strandat 30.651 kb on - strandat 30.862 kb on - strandat 30.862 kb on - strand, within CCNA_00028at 30.939 kb on - strand, within CCNA_00028at 30.961 kb on - strand, within CCNA_00028at 30.988 kb on - strand, within CCNA_00028at 31.272 kb on - strand, within CCNA_00028at 31.505 kb on - strand, within CCNA_00028at 31.675 kb on - strand, within CCNA_00028at 32.182 kb on - strand, within CCNA_00028at 32.656 kb on - strandat 32.808 kb on - strandat 32.841 kb on + strandat 32.936 kb on - strandat 32.945 kb on + strandat 32.947 kb on - strandat 32.947 kb on - strandat 32.965 kb on + strandat 32.966 kb on - strandat 32.972 kb on + strandat 32.991 kb on + strand, within CCNA_00029at 32.991 kb on - strand, within CCNA_00029at 33.022 kb on - strand, within CCNA_00029at 33.026 kb on - strand, within CCNA_00029at 33.027 kb on + strand, within CCNA_00029at 33.036 kb on - strand, within CCNA_00029at 33.045 kb on + strand, within CCNA_00029at 33.083 kb on + strand, within CCNA_00029at 33.088 kb on + strand, within CCNA_00029at 33.093 kb on + strand, within CCNA_00029at 33.096 kb on - strand, within CCNA_00029at 33.108 kb on + strand, within CCNA_00029at 33.116 kb on - strand, within CCNA_00029at 33.116 kb on - strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.128 kb on + strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.136 kb on - strand, within CCNA_00029at 33.138 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.228 kb on + strand, within CCNA_00029at 33.236 kb on - strand, within CCNA_00029at 33.237 kb on - strand, within CCNA_00029at 33.237 kb on - strand, within CCNA_00029at 33.260 kb on - strand, within CCNA_00029at 33.279 kb on + strand, within CCNA_00029at 33.292 kb on - strand, within CCNA_00029at 33.298 kb on + strand, within CCNA_00029at 33.298 kb on + strand, within CCNA_00029at 33.335 kb on - strandat 33.358 kb on - strand, within CCNA_00029at 33.367 kb on + strand, within CCNA_00029at 33.370 kb on - strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.379 kb on + strand, within CCNA_00029at 33.382 kb on - strand, within CCNA_00029at 33.384 kb on + strand, within CCNA_00029at 33.403 kb on - strand, within CCNA_00029at 33.456 kb on + strand, within CCNA_00029at 33.472 kb on - strand, within CCNA_00029at 33.499 kb on + strandat 33.499 kb on + strandat 33.499 kb on - strandat 33.499 kb on - strandat 33.562 kb on - strandat 33.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM
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29,345 + -0.3
29,345 + +0.2
29,345 + -0.4
29,353 - -0.3
29,449 - -0.2
29,471 - +0.9
29,508 - +1.0
29,586 - CCNA_00027 0.12 -0.6
29,833 + CCNA_00027 0.48 +1.6
29,841 - CCNA_00027 0.49 +1.6
30,037 - CCNA_00027 0.78 +1.6
30,189 - -0.2
30,251 + +1.0
30,284 + -1.8
30,292 - +2.0
30,294 + -0.9
30,302 - +1.9
30,312 + -2.1
30,320 - +2.6
30,322 + -1.8
30,322 + +0.5
30,322 + -0.2
30,330 - +3.5
30,330 - +3.3
30,330 - +3.4
30,330 - +3.2
30,330 - +3.5
30,335 + -0.7
30,651 - +3.0
30,862 - +3.2
30,862 - CCNA_00028 0.23 +3.1
30,939 - CCNA_00028 0.26 +2.1
30,961 - CCNA_00028 0.27 +2.6
30,988 - CCNA_00028 0.28 +2.3
31,272 - CCNA_00028 0.40 +2.4
31,505 - CCNA_00028 0.50 +2.1
31,675 - CCNA_00028 0.58 +2.5
32,182 - CCNA_00028 0.79 +3.4
32,656 - +0.9
32,808 - -1.0
32,841 + +1.9
32,936 - +1.2
32,945 + +1.9
32,947 - -0.2
32,947 - -0.1
32,965 + -0.9
32,966 - +2.1
32,972 + +0.8
32,991 + CCNA_00029 0.12 -1.5
32,991 - CCNA_00029 0.12 +2.4
33,022 - CCNA_00029 0.17 +0.4
33,026 - CCNA_00029 0.18 -2.3
33,027 + CCNA_00029 0.18 +1.3
33,036 - CCNA_00029 0.19 +0.1
33,045 + CCNA_00029 0.21 +1.6
33,083 + CCNA_00029 0.27 +1.6
33,088 + CCNA_00029 0.27 +0.5
33,093 + CCNA_00029 0.28 +0.4
33,096 - CCNA_00029 0.29 -1.1
33,108 + CCNA_00029 0.31 +0.2
33,116 - CCNA_00029 0.32 +0.3
33,116 - CCNA_00029 0.32 +0.5
33,128 + CCNA_00029 0.34 -0.2
33,128 + CCNA_00029 0.34 -0.9
33,128 + CCNA_00029 0.34 -0.5
33,128 + CCNA_00029 0.34 +0.6
33,136 - CCNA_00029 0.35 +2.6
33,136 - CCNA_00029 0.35 -1.1
33,136 - CCNA_00029 0.35 +1.0
33,136 - CCNA_00029 0.35 -1.1
33,136 - CCNA_00029 0.35 -1.6
33,138 + CCNA_00029 0.35 -1.1
33,228 + CCNA_00029 0.50 -0.7
33,228 + CCNA_00029 0.50 +1.8
33,228 + CCNA_00029 0.50 +1.5
33,228 + CCNA_00029 0.50 +0.0
33,236 - CCNA_00029 0.51 +0.2
33,237 - CCNA_00029 0.51 +0.2
33,237 - CCNA_00029 0.51 +0.8
33,260 - CCNA_00029 0.55 +0.8
33,279 + CCNA_00029 0.58 +2.0
33,292 - CCNA_00029 0.60 +0.3
33,298 + CCNA_00029 0.61 +0.6
33,298 + CCNA_00029 0.61 +0.5
33,335 - +1.2
33,358 - CCNA_00029 0.70 -0.9
33,367 + CCNA_00029 0.72 +0.7
33,370 - CCNA_00029 0.72 +0.8
33,379 + CCNA_00029 0.73 +0.3
33,379 + CCNA_00029 0.73 +1.7
33,382 - CCNA_00029 0.74 -1.3
33,384 + CCNA_00029 0.74 +2.0
33,403 - CCNA_00029 0.77 +0.9
33,456 + CCNA_00029 0.86 +1.9
33,472 - CCNA_00029 0.88 +1.5
33,499 + -1.5
33,499 + -0.5
33,499 - +1.4
33,499 - +0.1
33,562 - -0.9
33,565 + +3.6

Or see this region's nucleotide sequence