Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00006 and CCNA_00007 are separated by 149 nucleotides CCNA_00007 and CCNA_00008 are separated by 400 nucleotides CCNA_00008 and CCNA_00009 are separated by 61 nucleotides
CCNA_00006: CCNA_00006 - enoyl-CoA hydratase, at 4,022 to 4,810
_00006
CCNA_00007: CCNA_00007 - SSU ribosomal protein S20P, at 4,960 to 5,235
_00007
CCNA_00008: CCNA_00008 - chromosome replication initiator protein DnaA, at 5,636 to 7,108
_00008
CCNA_00009: CCNA_00009 - alkylated DNA repair protein AlkB, at 7,170 to 7,790
_00009
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4.661 kb on + strand, within CCNA_00006 at 4.666 kb on + strand, within CCNA_00006 at 4.674 kb on - strand, within CCNA_00006 at 4.688 kb on + strand, within CCNA_00006 at 4.783 kb on + strand at 4.783 kb on + strand at 4.783 kb on + strand at 4.783 kb on - strand at 4.791 kb on - strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.803 kb on - strand at 4.803 kb on - strand at 4.803 kb on - strand at 4.815 kb on + strand at 4.863 kb on + strand at 5.471 kb on + strand at 5.471 kb on + strand at 5.471 kb on + strand at 5.514 kb on - strand at 5.598 kb on + strand at 7.124 kb on + strand at 7.137 kb on - strand at 7.139 kb on + strand at 7.185 kb on - strand at 7.193 kb on + strand at 7.198 kb on + strand at 7.227 kb on + strand at 7.279 kb on + strand, within CCNA_00009 at 7.287 kb on - strand, within CCNA_00009 at 7.299 kb on - strand, within CCNA_00009 at 7.354 kb on + strand, within CCNA_00009 at 7.359 kb on + strand, within CCNA_00009 at 7.367 kb on - strand, within CCNA_00009 at 7.377 kb on - strand, within CCNA_00009 at 7.384 kb on + strand, within CCNA_00009 at 7.440 kb on - strand, within CCNA_00009 at 7.459 kb on + strand, within CCNA_00009 at 7.467 kb on - strand, within CCNA_00009 at 7.467 kb on - strand, within CCNA_00009 at 7.497 kb on + strand, within CCNA_00009 at 7.497 kb on + strand, within CCNA_00009 at 7.505 kb on - strand at 7.505 kb on - strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.526 kb on - strand, within CCNA_00009 at 7.548 kb on - strand, within CCNA_00009 at 7.548 kb on - strand, within CCNA_00009 at 7.566 kb on + strand, within CCNA_00009 at 7.603 kb on - strand, within CCNA_00009 at 7.622 kb on + strand, within CCNA_00009 at 7.643 kb on + strand, within CCNA_00009 at 7.657 kb on + strand, within CCNA_00009 at 7.657 kb on + strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.692 kb on + strand, within CCNA_00009 at 7.692 kb on + strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.742 kb on - strand at 7.758 kb on + strand at 7.759 kb on - strand at 7.759 kb on - strand at 7.763 kb on + strand at 7.770 kb on + strand at 7.796 kb on - strand at 7.807 kb on + strand at 7.930 kb on + strand at 7.930 kb on + strand at 7.931 kb on + strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.939 kb on - strand at 7.939 kb on - strand at 7.958 kb on - strand at 7.989 kb on - strand at 7.999 kb on + strand at 8.002 kb on - strand at 8.039 kb on + strand at 8.039 kb on + strand at 8.047 kb on - strand at 8.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 4,661 + CCNA_00006 0.81 -2.5 4,666 + CCNA_00006 0.82 -0.8 4,674 - CCNA_00006 0.83 +0.3 4,688 + CCNA_00006 0.84 +0.0 4,783 + -0.5 4,783 + +0.7 4,783 + -0.2 4,783 - -0.4 4,791 - -0.7 4,795 + -1.6 4,795 + -0.5 4,795 + -0.8 4,795 + -0.6 4,795 + -2.3 4,795 + -1.0 4,795 + +0.5 4,795 + -0.9 4,803 - +0.1 4,803 - +0.3 4,803 - +3.0 4,815 + +1.6 4,863 + +3.2 5,471 + +3.8 5,471 + +4.6 5,471 + +3.2 5,514 - +0.4 5,598 + +3.3 7,124 + +4.4 7,137 - -0.9 7,139 + +3.0 7,185 - +0.6 7,193 + +2.8 7,198 + -0.5 7,227 + +0.8 7,279 + CCNA_00009 0.18 -0.5 7,287 - CCNA_00009 0.19 -1.5 7,299 - CCNA_00009 0.21 -0.2 7,354 + CCNA_00009 0.30 +0.6 7,359 + CCNA_00009 0.30 +2.8 7,367 - CCNA_00009 0.32 +1.0 7,377 - CCNA_00009 0.33 -0.2 7,384 + CCNA_00009 0.34 +0.5 7,440 - CCNA_00009 0.43 +0.9 7,459 + CCNA_00009 0.47 +1.1 7,467 - CCNA_00009 0.48 -0.2 7,467 - CCNA_00009 0.48 +0.6 7,497 + CCNA_00009 0.53 +0.6 7,497 + CCNA_00009 0.53 -0.0 7,505 - +4.0 7,505 - CCNA_00009 0.54 +1.7 7,507 + CCNA_00009 0.54 -1.1 7,507 + CCNA_00009 0.54 +1.4 7,507 + CCNA_00009 0.54 +1.8 7,526 - CCNA_00009 0.57 +0.7 7,548 - CCNA_00009 0.61 +0.9 7,548 - CCNA_00009 0.61 +0.8 7,566 + CCNA_00009 0.64 -0.2 7,603 - CCNA_00009 0.70 -1.0 7,622 + CCNA_00009 0.73 -0.8 7,643 + CCNA_00009 0.76 -1.3 7,657 + CCNA_00009 0.78 +2.4 7,657 + CCNA_00009 0.78 +0.6 7,665 - CCNA_00009 0.80 -0.3 7,665 - CCNA_00009 0.80 -1.7 7,665 - CCNA_00009 0.80 +1.2 7,665 - CCNA_00009 0.80 +0.5 7,692 + CCNA_00009 0.84 -0.4 7,692 + CCNA_00009 0.84 -1.5 7,700 - CCNA_00009 0.85 +0.4 7,700 - CCNA_00009 0.85 -0.3 7,700 - CCNA_00009 0.85 -0.8 7,742 - +0.4 7,758 + -0.8 7,759 - -1.7 7,759 - -0.6 7,763 + -0.0 7,770 + -0.3 7,796 - -0.0 7,807 + +0.6 7,930 + +1.2 7,930 + +2.2 7,931 + +1.5 7,938 - +1.0 7,938 - +0.6 7,938 - +1.0 7,938 - -2.3 7,939 - -1.4 7,939 - +1.0 7,958 - +3.1 7,989 - +3.5 7,999 + +3.7 8,002 - +0.4 8,039 + +4.2 8,039 + +4.1 8,047 - +1.9 8,055 - +1.4
Or see this region's nucleotide sequence