Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS21765 and ABZR86_RS21770 are separated by 68 nucleotides ABZR86_RS21770 and ABZR86_RS21775 are separated by 23 nucleotides ABZR86_RS21775 and ABZR86_RS21780 are separated by 199 nucleotides
ABZR86_RS21765: ABZR86_RS21765 - amidase, at 327,041 to 328,666
_RS21765
ABZR86_RS21770: ABZR86_RS21770 - class II 3-deoxy-7-phosphoheptulonate synthase, at 328,735 to 330,108
_RS21770
ABZR86_RS21775: ABZR86_RS21775 - disulfide bond formation protein B, at 330,132 to 330,635
_RS21775
ABZR86_RS21780: ABZR86_RS21780 - 50S ribosomal protein L17, at 330,835 to 331,218
_RS21780
Position (kb) 328
329
330
331 Strain fitness (log2 ratio) -1
0
1
2 at 328.082 kb on + strand, within ABZR86_RS21765 at 328.082 kb on + strand, within ABZR86_RS21765 at 328.082 kb on + strand, within ABZR86_RS21765 at 328.082 kb on + strand, within ABZR86_RS21765 at 328.083 kb on - strand, within ABZR86_RS21765 at 328.083 kb on - strand, within ABZR86_RS21765 at 328.083 kb on - strand, within ABZR86_RS21765 at 328.099 kb on + strand, within ABZR86_RS21765 at 328.099 kb on + strand, within ABZR86_RS21765 at 328.100 kb on - strand, within ABZR86_RS21765 at 328.100 kb on - strand, within ABZR86_RS21765 at 328.109 kb on + strand, within ABZR86_RS21765 at 328.256 kb on + strand, within ABZR86_RS21765 at 328.256 kb on + strand, within ABZR86_RS21765 at 328.256 kb on + strand, within ABZR86_RS21765 at 328.256 kb on + strand, within ABZR86_RS21765 at 328.257 kb on - strand, within ABZR86_RS21765 at 328.257 kb on - strand, within ABZR86_RS21765 at 328.257 kb on - strand, within ABZR86_RS21765 at 328.556 kb on + strand at 328.557 kb on - strand at 328.563 kb on - strand at 328.563 kb on - strand at 328.563 kb on - strand at 328.606 kb on + strand at 328.607 kb on - strand at 328.672 kb on - strand at 328.672 kb on - strand at 328.672 kb on - strand at 328.679 kb on - strand at 328.679 kb on - strand at 328.736 kb on + strand at 328.737 kb on - strand at 328.799 kb on + strand at 328.799 kb on + strand at 328.800 kb on - strand at 328.800 kb on - strand at 328.800 kb on - strand at 328.830 kb on - strand at 328.978 kb on - strand, within ABZR86_RS21770 at 329.001 kb on - strand, within ABZR86_RS21770 at 329.022 kb on + strand, within ABZR86_RS21770 at 329.023 kb on - strand, within ABZR86_RS21770 at 329.252 kb on + strand, within ABZR86_RS21770 at 329.366 kb on + strand, within ABZR86_RS21770 at 329.366 kb on + strand, within ABZR86_RS21770 at 329.366 kb on + strand, within ABZR86_RS21770 at 329.366 kb on + strand, within ABZR86_RS21770 at 329.367 kb on - strand, within ABZR86_RS21770 at 329.532 kb on - strand, within ABZR86_RS21770 at 329.701 kb on + strand, within ABZR86_RS21770 at 329.744 kb on + strand, within ABZR86_RS21770 at 329.821 kb on + strand, within ABZR86_RS21770 at 329.822 kb on - strand, within ABZR86_RS21770 at 330.710 kb on - strand at 330.812 kb on + strand at 330.812 kb on + strand at 330.836 kb on + strand at 330.836 kb on + strand at 330.836 kb on + strand at 330.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 328,082 + ABZR86_RS21765 0.64 +0.4 328,082 + ABZR86_RS21765 0.64 +0.4 328,082 + ABZR86_RS21765 0.64 -1.4 328,082 + ABZR86_RS21765 0.64 +0.4 328,083 - ABZR86_RS21765 0.64 +0.6 328,083 - ABZR86_RS21765 0.64 +0.9 328,083 - ABZR86_RS21765 0.64 +0.7 328,099 + ABZR86_RS21765 0.65 +0.5 328,099 + ABZR86_RS21765 0.65 +0.4 328,100 - ABZR86_RS21765 0.65 +1.1 328,100 - ABZR86_RS21765 0.65 -0.3 328,109 + ABZR86_RS21765 0.66 +0.8 328,256 + ABZR86_RS21765 0.75 -0.4 328,256 + ABZR86_RS21765 0.75 +0.1 328,256 + ABZR86_RS21765 0.75 +1.8 328,256 + ABZR86_RS21765 0.75 -0.6 328,257 - ABZR86_RS21765 0.75 +0.2 328,257 - ABZR86_RS21765 0.75 -1.4 328,257 - ABZR86_RS21765 0.75 +0.6 328,556 + +0.8 328,557 - -0.1 328,563 - -1.5 328,563 - -0.8 328,563 - +0.1 328,606 + -1.0 328,607 - -0.3 328,672 - -0.2 328,672 - -0.5 328,672 - +0.3 328,679 - -0.0 328,679 - +1.5 328,736 + +0.6 328,737 - +1.0 328,799 + +0.3 328,799 + -0.5 328,800 - -0.1 328,800 - -0.9 328,800 - -0.7 328,830 - +0.4 328,978 - ABZR86_RS21770 0.18 +1.1 329,001 - ABZR86_RS21770 0.19 +0.1 329,022 + ABZR86_RS21770 0.21 -0.6 329,023 - ABZR86_RS21770 0.21 -1.2 329,252 + ABZR86_RS21770 0.38 -0.3 329,366 + ABZR86_RS21770 0.46 +0.3 329,366 + ABZR86_RS21770 0.46 +0.1 329,366 + ABZR86_RS21770 0.46 +0.3 329,366 + ABZR86_RS21770 0.46 +0.1 329,367 - ABZR86_RS21770 0.46 +1.2 329,532 - ABZR86_RS21770 0.58 -1.0 329,701 + ABZR86_RS21770 0.70 -0.7 329,744 + ABZR86_RS21770 0.73 -0.4 329,821 + ABZR86_RS21770 0.79 +0.9 329,822 - ABZR86_RS21770 0.79 -0.4 330,710 - -1.5 330,812 + +0.1 330,812 + -1.7 330,836 + +0.6 330,836 + +0.4 330,836 + -0.6 330,837 - +0.3
Or see this region's nucleotide sequence