Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS21400 and ABZR86_RS21405 are separated by 52 nucleotides ABZR86_RS21405 and ABZR86_RS21410 overlap by 4 nucleotides ABZR86_RS21410 and ABZR86_RS21415 are separated by 223 nucleotides ABZR86_RS21415 and ABZR86_RS21420 are separated by 159 nucleotides
ABZR86_RS21400: ABZR86_RS21400 - DNA topoisomerase IV subunit A, at 245,532 to 247,772
_RS21400
ABZR86_RS21405: ABZR86_RS21405 - biotin--protein ligase, at 247,825 to 248,115
_RS21405
ABZR86_RS21410: ABZR86_RS21410 - biotin/lipoate A/B protein ligase family protein, at 248,112 to 248,888
_RS21410
ABZR86_RS21415: ABZR86_RS21415 - hypothetical protein, at 249,112 to 249,600
_RS21415
ABZR86_RS21420: ABZR86_RS21420 - alpha/beta fold hydrolase, at 249,760 to 250,602
_RS21420
Position (kb) 248
249 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 247.837 kb on + strand at 247.837 kb on + strand at 247.978 kb on + strand, within ABZR86_RS21405 at 247.978 kb on + strand, within ABZR86_RS21405 at 247.979 kb on - strand, within ABZR86_RS21405 at 248.047 kb on + strand, within ABZR86_RS21405 at 248.047 kb on + strand, within ABZR86_RS21405 at 248.047 kb on + strand, within ABZR86_RS21405 at 248.047 kb on + strand, within ABZR86_RS21405 at 248.047 kb on + strand, within ABZR86_RS21405 at 248.048 kb on - strand, within ABZR86_RS21405 at 248.403 kb on + strand, within ABZR86_RS21410 at 248.404 kb on - strand, within ABZR86_RS21410 at 248.412 kb on + strand, within ABZR86_RS21410 at 248.412 kb on + strand, within ABZR86_RS21410 at 248.413 kb on - strand, within ABZR86_RS21410 at 248.413 kb on - strand, within ABZR86_RS21410 at 248.413 kb on - strand, within ABZR86_RS21410 at 248.458 kb on - strand, within ABZR86_RS21410 at 248.458 kb on - strand, within ABZR86_RS21410 at 248.517 kb on + strand, within ABZR86_RS21410 at 248.575 kb on + strand, within ABZR86_RS21410 at 248.575 kb on + strand, within ABZR86_RS21410 at 248.575 kb on + strand, within ABZR86_RS21410 at 248.576 kb on - strand, within ABZR86_RS21410 at 248.617 kb on - strand, within ABZR86_RS21410 at 248.617 kb on - strand, within ABZR86_RS21410 at 248.886 kb on + strand at 248.911 kb on - strand at 248.911 kb on - strand at 248.997 kb on + strand at 248.997 kb on + strand at 248.997 kb on + strand at 248.998 kb on - strand at 248.998 kb on - strand at 249.061 kb on - strand at 249.085 kb on + strand at 249.303 kb on + strand, within ABZR86_RS21415 at 249.303 kb on + strand, within ABZR86_RS21415 at 249.341 kb on - strand, within ABZR86_RS21415 at 249.341 kb on - strand, within ABZR86_RS21415 at 249.341 kb on - strand, within ABZR86_RS21415 at 249.343 kb on + strand, within ABZR86_RS21415 at 249.343 kb on + strand, within ABZR86_RS21415 at 249.343 kb on + strand, within ABZR86_RS21415 at 249.344 kb on - strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.424 kb on + strand, within ABZR86_RS21415 at 249.425 kb on - strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.496 kb on + strand, within ABZR86_RS21415 at 249.497 kb on - strand, within ABZR86_RS21415 at 249.497 kb on - strand, within ABZR86_RS21415 at 249.497 kb on - strand, within ABZR86_RS21415 at 249.605 kb on + strand at 249.605 kb on + strand at 249.605 kb on + strand at 249.606 kb on - strand at 249.655 kb on + strand at 249.692 kb on + strand at 249.692 kb on + strand at 249.692 kb on + strand at 249.693 kb on - strand at 249.693 kb on - strand at 249.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 247,837 + +0.2 247,837 + +1.2 247,978 + ABZR86_RS21405 0.53 +0.8 247,978 + ABZR86_RS21405 0.53 +1.1 247,979 - ABZR86_RS21405 0.53 +0.0 248,047 + ABZR86_RS21405 0.76 -0.0 248,047 + ABZR86_RS21405 0.76 +0.8 248,047 + ABZR86_RS21405 0.76 +0.2 248,047 + ABZR86_RS21405 0.76 -0.1 248,047 + ABZR86_RS21405 0.76 +1.0 248,048 - ABZR86_RS21405 0.77 +0.7 248,403 + ABZR86_RS21410 0.37 +1.2 248,404 - ABZR86_RS21410 0.38 +0.1 248,412 + ABZR86_RS21410 0.39 -0.5 248,412 + ABZR86_RS21410 0.39 -0.7 248,413 - ABZR86_RS21410 0.39 -0.4 248,413 - ABZR86_RS21410 0.39 +0.5 248,413 - ABZR86_RS21410 0.39 -0.1 248,458 - ABZR86_RS21410 0.45 -0.0 248,458 - ABZR86_RS21410 0.45 +0.7 248,517 + ABZR86_RS21410 0.52 -0.1 248,575 + ABZR86_RS21410 0.60 +0.3 248,575 + ABZR86_RS21410 0.60 +0.9 248,575 + ABZR86_RS21410 0.60 -0.2 248,576 - ABZR86_RS21410 0.60 +0.4 248,617 - ABZR86_RS21410 0.65 +0.7 248,617 - ABZR86_RS21410 0.65 +0.6 248,886 + +1.2 248,911 - -1.1 248,911 - +0.0 248,997 + -0.8 248,997 + -0.2 248,997 + +0.2 248,998 - -0.3 248,998 - +0.7 249,061 - -0.9 249,085 + -0.6 249,303 + ABZR86_RS21415 0.39 -0.4 249,303 + ABZR86_RS21415 0.39 -0.2 249,341 - ABZR86_RS21415 0.47 +0.0 249,341 - ABZR86_RS21415 0.47 -0.9 249,341 - ABZR86_RS21415 0.47 -0.3 249,343 + ABZR86_RS21415 0.47 +0.1 249,343 + ABZR86_RS21415 0.47 -0.1 249,343 + ABZR86_RS21415 0.47 -1.5 249,344 - ABZR86_RS21415 0.47 +1.0 249,424 + ABZR86_RS21415 0.64 +0.6 249,424 + ABZR86_RS21415 0.64 -0.5 249,424 + ABZR86_RS21415 0.64 -0.6 249,424 + ABZR86_RS21415 0.64 -1.7 249,424 + ABZR86_RS21415 0.64 -0.5 249,424 + ABZR86_RS21415 0.64 -0.4 249,425 - ABZR86_RS21415 0.64 -0.5 249,496 + ABZR86_RS21415 0.79 +1.6 249,496 + ABZR86_RS21415 0.79 +0.4 249,496 + ABZR86_RS21415 0.79 +0.3 249,496 + ABZR86_RS21415 0.79 -1.1 249,496 + ABZR86_RS21415 0.79 -1.1 249,496 + ABZR86_RS21415 0.79 +0.8 249,497 - ABZR86_RS21415 0.79 +0.1 249,497 - ABZR86_RS21415 0.79 +0.8 249,497 - ABZR86_RS21415 0.79 -0.1 249,605 + +0.3 249,605 + -0.6 249,605 + -0.3 249,606 - -0.1 249,655 + +0.9 249,692 + +1.2 249,692 + -0.3 249,692 + -0.4 249,693 - +7.0 249,693 - +6.2 249,725 - +5.9
Or see this region's nucleotide sequence