Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS20565 and ABZR86_RS20570 are separated by 239 nucleotides ABZR86_RS20570 and ABZR86_RS20575 are separated by 144 nucleotides ABZR86_RS20575 and ABZR86_RS20580 are separated by 154 nucleotides
ABZR86_RS20565: ABZR86_RS20565 - S8 family serine peptidase, at 59,556 to 62,360
_RS20565
ABZR86_RS20570: ABZR86_RS20570 - acetyl-CoA C-acyltransferase, at 62,600 to 63,784
_RS20570
ABZR86_RS20575: ABZR86_RS20575 - hypothetical protein, at 63,929 to 64,291
_RS20575
ABZR86_RS20580: ABZR86_RS20580 - carboxyl transferase domain-containing protein, at 64,446 to 66,053
_RS20580
Position (kb)
62
63
64 Strain fitness (log2 ratio)
-1
0
1
2
3 at 61.683 kb on + strand, within ABZR86_RS20565 at 61.683 kb on + strand, within ABZR86_RS20565 at 61.684 kb on - strand, within ABZR86_RS20565 at 61.720 kb on + strand, within ABZR86_RS20565 at 61.720 kb on + strand, within ABZR86_RS20565 at 61.768 kb on + strand, within ABZR86_RS20565 at 61.768 kb on + strand, within ABZR86_RS20565 at 62.047 kb on + strand, within ABZR86_RS20565 at 62.047 kb on + strand, within ABZR86_RS20565 at 62.047 kb on + strand, within ABZR86_RS20565 at 62.047 kb on + strand, within ABZR86_RS20565 at 62.048 kb on - strand, within ABZR86_RS20565 at 62.165 kb on + strand at 62.541 kb on + strand at 63.056 kb on + strand, within ABZR86_RS20570 at 63.104 kb on + strand, within ABZR86_RS20570 at 63.104 kb on + strand, within ABZR86_RS20570 at 63.105 kb on - strand, within ABZR86_RS20570 at 63.105 kb on - strand, within ABZR86_RS20570 at 63.105 kb on - strand, within ABZR86_RS20570 at 63.846 kb on + strand at 63.847 kb on - strand at 63.881 kb on + strand at 63.881 kb on + strand at 63.884 kb on + strand at 63.884 kb on + strand at 63.884 kb on + strand at 63.884 kb on + strand at 63.884 kb on + strand at 63.888 kb on - strand at 63.888 kb on - strand at 63.888 kb on - strand at 63.948 kb on + strand at 63.949 kb on - strand at 63.949 kb on - strand at 64.290 kb on - strand at 64.689 kb on + strand, within ABZR86_RS20580 at 64.689 kb on + strand, within ABZR86_RS20580 at 64.690 kb on - strand, within ABZR86_RS20580 at 64.690 kb on - strand, within ABZR86_RS20580 at 64.690 kb on - strand, within ABZR86_RS20580
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 61,683 + ABZR86_RS20565 0.76 -0.3 61,683 + ABZR86_RS20565 0.76 -0.4 61,684 - ABZR86_RS20565 0.76 -0.3 61,720 + ABZR86_RS20565 0.77 +0.4 61,720 + ABZR86_RS20565 0.77 +0.2 61,768 + ABZR86_RS20565 0.79 +0.1 61,768 + ABZR86_RS20565 0.79 -1.1 62,047 + ABZR86_RS20565 0.89 -0.2 62,047 + ABZR86_RS20565 0.89 +0.4 62,047 + ABZR86_RS20565 0.89 +0.4 62,047 + ABZR86_RS20565 0.89 -1.6 62,048 - ABZR86_RS20565 0.89 -0.1 62,165 + +0.2 62,541 + -0.4 63,056 + ABZR86_RS20570 0.38 -1.6 63,104 + ABZR86_RS20570 0.43 -0.4 63,104 + ABZR86_RS20570 0.43 -0.7 63,105 - ABZR86_RS20570 0.43 +1.3 63,105 - ABZR86_RS20570 0.43 +1.6 63,105 - ABZR86_RS20570 0.43 +0.3 63,846 + -0.5 63,847 - +1.7 63,881 + +0.6 63,881 + +1.6 63,884 + +1.3 63,884 + -0.0 63,884 + +1.0 63,884 + +0.9 63,884 + -0.3 63,888 - +0.7 63,888 - +0.9 63,888 - -0.1 63,948 + +1.2 63,949 - +1.8 63,949 - +1.1 64,290 - +1.2 64,689 + ABZR86_RS20580 0.15 -0.1 64,689 + ABZR86_RS20580 0.15 -0.4 64,690 - ABZR86_RS20580 0.15 -0.1 64,690 - ABZR86_RS20580 0.15 +2.8 64,690 - ABZR86_RS20580 0.15 -0.1
Or see this region's nucleotide sequence