Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19255 and ABZR86_RS19260 are separated by 473 nucleotides ABZR86_RS19260 and ABZR86_RS19265 are separated by 133 nucleotides ABZR86_RS19265 and ABZR86_RS19270 are separated by 10 nucleotides
ABZR86_RS19255: ABZR86_RS19255 - DUF2127 domain-containing protein, at 308,090 to 308,599
_RS19255
ABZR86_RS19260: ABZR86_RS19260 - filamentous hemagglutinin N-terminal domain-containing protein, at 309,073 to 311,274
_RS19260
ABZR86_RS19265: ABZR86_RS19265 - YbdD/YjiX family protein, at 311,408 to 311,602
_RS19265
ABZR86_RS19270: ABZR86_RS19270 - carbon starvation CstA family protein, at 311,613 to 313,688
_RS19270
Position (kb) 309
310
311
312 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 308.091 kb on + strand at 308.091 kb on + strand at 308.091 kb on + strand at 308.152 kb on + strand, within ABZR86_RS19255 at 308.206 kb on - strand, within ABZR86_RS19255 at 308.277 kb on - strand, within ABZR86_RS19255 at 308.277 kb on - strand, within ABZR86_RS19255 at 308.311 kb on - strand, within ABZR86_RS19255 at 308.514 kb on + strand, within ABZR86_RS19255 at 308.514 kb on + strand, within ABZR86_RS19255 at 308.515 kb on - strand, within ABZR86_RS19255 at 308.515 kb on - strand, within ABZR86_RS19255 at 308.612 kb on - strand at 308.788 kb on + strand at 308.788 kb on + strand at 308.789 kb on - strand at 309.051 kb on + strand at 309.052 kb on - strand at 309.066 kb on + strand at 309.066 kb on + strand at 309.066 kb on + strand at 309.067 kb on - strand at 309.094 kb on + strand at 309.095 kb on - strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.181 kb on - strand at 309.195 kb on + strand at 309.196 kb on - strand at 309.221 kb on + strand at 309.221 kb on + strand at 309.221 kb on + strand at 309.222 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand at 309.280 kb on - strand at 309.625 kb on + strand, within ABZR86_RS19260 at 309.626 kb on - strand, within ABZR86_RS19260 at 309.677 kb on + strand, within ABZR86_RS19260 at 309.677 kb on + strand, within ABZR86_RS19260 at 309.678 kb on - strand, within ABZR86_RS19260 at 309.678 kb on - strand, within ABZR86_RS19260 at 309.704 kb on - strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.790 kb on + strand, within ABZR86_RS19260 at 309.791 kb on - strand, within ABZR86_RS19260 at 309.923 kb on + strand, within ABZR86_RS19260 at 310.120 kb on + strand, within ABZR86_RS19260 at 310.120 kb on + strand, within ABZR86_RS19260 at 310.120 kb on + strand, within ABZR86_RS19260 at 310.120 kb on + strand, within ABZR86_RS19260 at 310.121 kb on - strand, within ABZR86_RS19260 at 310.141 kb on - strand, within ABZR86_RS19260 at 310.256 kb on - strand, within ABZR86_RS19260 at 310.256 kb on - strand, within ABZR86_RS19260 at 310.264 kb on + strand, within ABZR86_RS19260 at 310.273 kb on + strand, within ABZR86_RS19260 at 310.273 kb on + strand, within ABZR86_RS19260 at 310.274 kb on - strand, within ABZR86_RS19260 at 310.309 kb on - strand, within ABZR86_RS19260 at 310.417 kb on + strand, within ABZR86_RS19260 at 310.433 kb on - strand, within ABZR86_RS19260 at 310.458 kb on + strand, within ABZR86_RS19260 at 310.509 kb on + strand, within ABZR86_RS19260 at 310.509 kb on + strand, within ABZR86_RS19260 at 310.510 kb on - strand, within ABZR86_RS19260 at 310.601 kb on - strand, within ABZR86_RS19260 at 310.606 kb on + strand, within ABZR86_RS19260 at 310.606 kb on + strand, within ABZR86_RS19260 at 310.606 kb on + strand, within ABZR86_RS19260 at 310.606 kb on + strand, within ABZR86_RS19260 at 310.606 kb on + strand, within ABZR86_RS19260 at 310.607 kb on - strand, within ABZR86_RS19260 at 310.607 kb on - strand, within ABZR86_RS19260 at 310.607 kb on - strand, within ABZR86_RS19260 at 310.607 kb on - strand, within ABZR86_RS19260 at 310.607 kb on - strand, within ABZR86_RS19260 at 310.612 kb on + strand, within ABZR86_RS19260 at 310.624 kb on + strand, within ABZR86_RS19260 at 310.625 kb on - strand, within ABZR86_RS19260 at 310.625 kb on - strand, within ABZR86_RS19260 at 310.636 kb on + strand, within ABZR86_RS19260 at 310.637 kb on - strand, within ABZR86_RS19260 at 310.637 kb on - strand, within ABZR86_RS19260 at 310.648 kb on + strand, within ABZR86_RS19260 at 310.648 kb on + strand, within ABZR86_RS19260 at 310.648 kb on + strand, within ABZR86_RS19260 at 310.648 kb on + strand, within ABZR86_RS19260 at 310.648 kb on + strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.649 kb on - strand, within ABZR86_RS19260 at 310.735 kb on + strand, within ABZR86_RS19260 at 311.083 kb on - strand at 311.182 kb on + strand at 311.182 kb on + strand at 311.183 kb on - strand at 311.183 kb on - strand at 311.183 kb on - strand at 311.183 kb on - strand at 311.217 kb on + strand at 311.240 kb on - strand at 311.272 kb on + strand at 311.273 kb on - strand at 311.273 kb on - strand at 311.273 kb on - strand at 311.273 kb on - strand at 311.273 kb on - strand at 311.273 kb on - strand at 311.436 kb on + strand, within ABZR86_RS19265 at 311.436 kb on + strand, within ABZR86_RS19265 at 311.437 kb on - strand, within ABZR86_RS19265 at 311.437 kb on - strand, within ABZR86_RS19265 at 311.472 kb on + strand, within ABZR86_RS19265 at 311.472 kb on + strand, within ABZR86_RS19265 at 311.473 kb on - strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.520 kb on + strand, within ABZR86_RS19265 at 311.530 kb on - strand, within ABZR86_RS19265 at 311.530 kb on - strand, within ABZR86_RS19265 at 311.530 kb on - strand, within ABZR86_RS19265 at 311.530 kb on - strand, within ABZR86_RS19265 at 311.644 kb on + strand at 311.644 kb on + strand at 311.644 kb on + strand at 311.645 kb on - strand at 311.645 kb on - strand at 311.645 kb on - strand at 311.764 kb on + strand at 311.764 kb on + strand at 311.764 kb on + strand at 311.765 kb on - strand at 311.765 kb on - strand at 311.765 kb on - strand at 311.890 kb on + strand, within ABZR86_RS19270 at 311.890 kb on + strand, within ABZR86_RS19270 at 311.890 kb on + strand, within ABZR86_RS19270 at 311.890 kb on + strand, within ABZR86_RS19270 at 311.890 kb on + strand, within ABZR86_RS19270 at 311.890 kb on + strand, within ABZR86_RS19270 at 311.891 kb on - strand, within ABZR86_RS19270 at 311.953 kb on + strand, within ABZR86_RS19270 at 312.050 kb on + strand, within ABZR86_RS19270 at 312.050 kb on + strand, within ABZR86_RS19270 at 312.050 kb on + strand, within ABZR86_RS19270 at 312.051 kb on - strand, within ABZR86_RS19270 at 312.051 kb on - strand, within ABZR86_RS19270 at 312.051 kb on - strand, within ABZR86_RS19270 at 312.051 kb on - strand, within ABZR86_RS19270 at 312.085 kb on + strand, within ABZR86_RS19270 at 312.086 kb on - strand, within ABZR86_RS19270 at 312.265 kb on + strand, within ABZR86_RS19270 at 312.265 kb on + strand, within ABZR86_RS19270
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 308,091 + +0.7 308,091 + +0.9 308,091 + -0.1 308,152 + ABZR86_RS19255 0.12 -0.3 308,206 - ABZR86_RS19255 0.23 +0.5 308,277 - ABZR86_RS19255 0.37 -1.0 308,277 - ABZR86_RS19255 0.37 +0.9 308,311 - ABZR86_RS19255 0.43 -0.3 308,514 + ABZR86_RS19255 0.83 -0.8 308,514 + ABZR86_RS19255 0.83 +0.1 308,515 - ABZR86_RS19255 0.83 +3.3 308,515 - ABZR86_RS19255 0.83 -2.2 308,612 - +1.1 308,788 + -0.8 308,788 + -1.2 308,789 - +1.3 309,051 + +0.2 309,052 - -0.4 309,066 + +0.1 309,066 + +0.1 309,066 + -0.0 309,067 - +1.0 309,094 + -1.2 309,095 - -0.1 309,180 + +1.7 309,180 + -0.1 309,181 - -0.2 309,195 + -0.0 309,196 - +0.2 309,221 + -0.9 309,221 + -1.9 309,221 + +0.4 309,222 - +0.1 309,280 - +0.2 309,280 - -0.0 309,280 - +1.1 309,280 - +1.1 309,625 + ABZR86_RS19260 0.25 -0.1 309,626 - ABZR86_RS19260 0.25 -0.2 309,677 + ABZR86_RS19260 0.27 -0.6 309,677 + ABZR86_RS19260 0.27 -0.4 309,678 - ABZR86_RS19260 0.27 -0.9 309,678 - ABZR86_RS19260 0.27 +2.1 309,704 - ABZR86_RS19260 0.29 +0.1 309,790 + ABZR86_RS19260 0.33 +0.2 309,790 + ABZR86_RS19260 0.33 -0.6 309,790 + ABZR86_RS19260 0.33 +0.7 309,790 + ABZR86_RS19260 0.33 -1.9 309,790 + ABZR86_RS19260 0.33 +1.2 309,790 + ABZR86_RS19260 0.33 -0.2 309,791 - ABZR86_RS19260 0.33 +0.9 309,923 + ABZR86_RS19260 0.39 +0.8 310,120 + ABZR86_RS19260 0.48 +0.1 310,120 + ABZR86_RS19260 0.48 -1.0 310,120 + ABZR86_RS19260 0.48 -1.5 310,120 + ABZR86_RS19260 0.48 +0.1 310,121 - ABZR86_RS19260 0.48 +0.7 310,141 - ABZR86_RS19260 0.49 +1.4 310,256 - ABZR86_RS19260 0.54 -0.5 310,256 - ABZR86_RS19260 0.54 -0.8 310,264 + ABZR86_RS19260 0.54 -0.1 310,273 + ABZR86_RS19260 0.54 +0.1 310,273 + ABZR86_RS19260 0.54 -0.1 310,274 - ABZR86_RS19260 0.55 +1.0 310,309 - ABZR86_RS19260 0.56 +0.1 310,417 + ABZR86_RS19260 0.61 -0.3 310,433 - ABZR86_RS19260 0.62 -0.2 310,458 + ABZR86_RS19260 0.63 -0.7 310,509 + ABZR86_RS19260 0.65 +0.0 310,509 + ABZR86_RS19260 0.65 +0.9 310,510 - ABZR86_RS19260 0.65 -0.8 310,601 - ABZR86_RS19260 0.69 -0.3 310,606 + ABZR86_RS19260 0.70 +0.2 310,606 + ABZR86_RS19260 0.70 -0.7 310,606 + ABZR86_RS19260 0.70 +0.9 310,606 + ABZR86_RS19260 0.70 +0.5 310,606 + ABZR86_RS19260 0.70 +0.4 310,607 - ABZR86_RS19260 0.70 +1.2 310,607 - ABZR86_RS19260 0.70 -0.2 310,607 - ABZR86_RS19260 0.70 +0.8 310,607 - ABZR86_RS19260 0.70 -0.7 310,607 - ABZR86_RS19260 0.70 -0.9 310,612 + ABZR86_RS19260 0.70 -1.0 310,624 + ABZR86_RS19260 0.70 -0.4 310,625 - ABZR86_RS19260 0.70 -0.8 310,625 - ABZR86_RS19260 0.70 -0.5 310,636 + ABZR86_RS19260 0.71 +0.4 310,637 - ABZR86_RS19260 0.71 +2.2 310,637 - ABZR86_RS19260 0.71 +1.0 310,648 + ABZR86_RS19260 0.72 +0.9 310,648 + ABZR86_RS19260 0.72 -0.4 310,648 + ABZR86_RS19260 0.72 +0.8 310,648 + ABZR86_RS19260 0.72 -0.4 310,648 + ABZR86_RS19260 0.72 -0.6 310,649 - ABZR86_RS19260 0.72 +0.7 310,649 - ABZR86_RS19260 0.72 -0.7 310,649 - ABZR86_RS19260 0.72 -0.5 310,649 - ABZR86_RS19260 0.72 -0.5 310,649 - ABZR86_RS19260 0.72 -0.4 310,649 - ABZR86_RS19260 0.72 -1.1 310,649 - ABZR86_RS19260 0.72 -1.3 310,649 - ABZR86_RS19260 0.72 +0.6 310,649 - ABZR86_RS19260 0.72 +0.5 310,735 + ABZR86_RS19260 0.75 -2.4 311,083 - +0.5 311,182 + -0.0 311,182 + -0.7 311,183 - -0.7 311,183 - +1.5 311,183 - -0.5 311,183 - +0.2 311,217 + -0.9 311,240 - -1.2 311,272 + -0.3 311,273 - +0.5 311,273 - -1.0 311,273 - -0.9 311,273 - +0.5 311,273 - -1.8 311,273 - -1.0 311,436 + ABZR86_RS19265 0.14 -0.3 311,436 + ABZR86_RS19265 0.14 -0.6 311,437 - ABZR86_RS19265 0.15 -0.2 311,437 - ABZR86_RS19265 0.15 +0.6 311,472 + ABZR86_RS19265 0.33 +0.3 311,472 + ABZR86_RS19265 0.33 +0.2 311,473 - ABZR86_RS19265 0.33 -0.1 311,520 + ABZR86_RS19265 0.57 +0.3 311,520 + ABZR86_RS19265 0.57 -0.4 311,520 + ABZR86_RS19265 0.57 +0.0 311,520 + ABZR86_RS19265 0.57 +0.4 311,520 + ABZR86_RS19265 0.57 -0.3 311,520 + ABZR86_RS19265 0.57 +0.6 311,530 - ABZR86_RS19265 0.63 +0.7 311,530 - ABZR86_RS19265 0.63 +0.9 311,530 - ABZR86_RS19265 0.63 -0.5 311,530 - ABZR86_RS19265 0.63 +0.3 311,644 + -1.7 311,644 + +0.4 311,644 + -0.8 311,645 - +0.4 311,645 - -0.9 311,645 - -0.0 311,764 + -2.0 311,764 + +0.2 311,764 + -0.8 311,765 - -0.2 311,765 - +0.2 311,765 - +1.1 311,890 + ABZR86_RS19270 0.13 +0.2 311,890 + ABZR86_RS19270 0.13 +0.7 311,890 + ABZR86_RS19270 0.13 -0.1 311,890 + ABZR86_RS19270 0.13 +0.4 311,890 + ABZR86_RS19270 0.13 +1.3 311,890 + ABZR86_RS19270 0.13 +0.1 311,891 - ABZR86_RS19270 0.13 -0.7 311,953 + ABZR86_RS19270 0.16 +0.9 312,050 + ABZR86_RS19270 0.21 -0.5 312,050 + ABZR86_RS19270 0.21 -0.2 312,050 + ABZR86_RS19270 0.21 -1.0 312,051 - ABZR86_RS19270 0.21 -0.1 312,051 - ABZR86_RS19270 0.21 -0.3 312,051 - ABZR86_RS19270 0.21 -0.6 312,051 - ABZR86_RS19270 0.21 -1.2 312,085 + ABZR86_RS19270 0.23 -1.0 312,086 - ABZR86_RS19270 0.23 -0.4 312,265 + ABZR86_RS19270 0.31 +0.4 312,265 + ABZR86_RS19270 0.31 +0.4
Or see this region's nucleotide sequence