Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19205 and ABZR86_RS19210 are separated by 99 nucleotides ABZR86_RS19210 and ABZR86_RS19215 are separated by 190 nucleotides ABZR86_RS19215 and ABZR86_RS19220 are separated by 60 nucleotides
ABZR86_RS19205: ABZR86_RS19205 - low-specificity L-threonine aldolase, at 295,590 to 296,612
_RS19205
ABZR86_RS19210: ABZR86_RS19210 - polyhydroxyalkanoate depolymerase, at 296,712 to 297,965
_RS19210
ABZR86_RS19215: ABZR86_RS19215 - class I SAM-dependent methyltransferase, at 298,156 to 298,902
_RS19215
ABZR86_RS19220: ABZR86_RS19220 - RDD family protein, at 298,963 to 299,469
_RS19220
Position (kb) 296
297
298 Strain fitness (log2 ratio) -2
-1
0
1
2 at 295.821 kb on + strand, within ABZR86_RS19205 at 295.821 kb on + strand, within ABZR86_RS19205 at 295.821 kb on + strand, within ABZR86_RS19205 at 295.844 kb on + strand, within ABZR86_RS19205 at 295.844 kb on + strand, within ABZR86_RS19205 at 295.844 kb on + strand, within ABZR86_RS19205 at 295.844 kb on + strand, within ABZR86_RS19205 at 295.845 kb on - strand, within ABZR86_RS19205 at 295.845 kb on - strand, within ABZR86_RS19205 at 295.848 kb on + strand, within ABZR86_RS19205 at 295.849 kb on - strand, within ABZR86_RS19205 at 295.849 kb on - strand, within ABZR86_RS19205 at 295.849 kb on - strand, within ABZR86_RS19205 at 296.037 kb on + strand, within ABZR86_RS19205 at 296.512 kb on - strand at 296.619 kb on + strand at 296.620 kb on - strand at 296.620 kb on - strand at 296.701 kb on + strand at 296.701 kb on + strand at 296.701 kb on + strand at 296.701 kb on + strand at 296.701 kb on + strand at 296.701 kb on + strand at 296.702 kb on - strand at 296.702 kb on - strand at 296.702 kb on - strand at 296.702 kb on - strand at 296.761 kb on + strand at 296.762 kb on - strand at 297.031 kb on + strand, within ABZR86_RS19210 at 297.034 kb on + strand, within ABZR86_RS19210 at 297.034 kb on + strand, within ABZR86_RS19210 at 297.035 kb on - strand, within ABZR86_RS19210 at 297.064 kb on + strand, within ABZR86_RS19210 at 297.064 kb on + strand, within ABZR86_RS19210 at 297.064 kb on + strand, within ABZR86_RS19210 at 297.064 kb on + strand, within ABZR86_RS19210 at 297.065 kb on - strand, within ABZR86_RS19210 at 297.065 kb on - strand, within ABZR86_RS19210 at 297.065 kb on - strand, within ABZR86_RS19210 at 297.065 kb on - strand, within ABZR86_RS19210 at 297.073 kb on + strand, within ABZR86_RS19210 at 297.074 kb on - strand, within ABZR86_RS19210 at 297.128 kb on - strand, within ABZR86_RS19210 at 297.476 kb on - strand, within ABZR86_RS19210 at 297.476 kb on - strand, within ABZR86_RS19210 at 297.484 kb on + strand, within ABZR86_RS19210 at 297.484 kb on + strand, within ABZR86_RS19210 at 297.484 kb on + strand, within ABZR86_RS19210 at 297.485 kb on - strand, within ABZR86_RS19210 at 297.550 kb on + strand, within ABZR86_RS19210 at 297.550 kb on + strand, within ABZR86_RS19210 at 297.550 kb on + strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.551 kb on - strand, within ABZR86_RS19210 at 297.682 kb on + strand, within ABZR86_RS19210 at 297.682 kb on + strand, within ABZR86_RS19210 at 297.683 kb on - strand, within ABZR86_RS19210 at 297.796 kb on + strand, within ABZR86_RS19210 at 297.958 kb on + strand at 297.958 kb on + strand at 297.959 kb on - strand at 298.001 kb on + strand at 298.001 kb on + strand at 298.053 kb on + strand at 298.054 kb on - strand at 298.070 kb on + strand at 298.116 kb on + strand at 298.116 kb on + strand at 298.157 kb on + strand at 298.158 kb on - strand at 298.158 kb on - strand at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.307 kb on + strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.355 kb on + strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.605 kb on - strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.836 kb on - strand at 298.836 kb on - strand at 298.836 kb on - strand at 298.908 kb on + strand at 298.909 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 295,821 + ABZR86_RS19205 0.23 -0.1 295,821 + ABZR86_RS19205 0.23 -0.8 295,821 + ABZR86_RS19205 0.23 -0.7 295,844 + ABZR86_RS19205 0.25 +0.9 295,844 + ABZR86_RS19205 0.25 -1.6 295,844 + ABZR86_RS19205 0.25 -0.4 295,844 + ABZR86_RS19205 0.25 +0.2 295,845 - ABZR86_RS19205 0.25 -1.3 295,845 - ABZR86_RS19205 0.25 +0.4 295,848 + ABZR86_RS19205 0.25 -0.4 295,849 - ABZR86_RS19205 0.25 -1.2 295,849 - ABZR86_RS19205 0.25 -0.0 295,849 - ABZR86_RS19205 0.25 -0.6 296,037 + ABZR86_RS19205 0.44 +0.5 296,512 - +0.3 296,619 + +0.4 296,620 - -1.4 296,620 - -0.6 296,701 + -0.2 296,701 + -0.6 296,701 + -1.1 296,701 + -0.8 296,701 + -0.5 296,701 + +0.5 296,702 - -2.0 296,702 - -0.5 296,702 - -0.2 296,702 - +1.6 296,761 + +0.8 296,762 - -0.3 297,031 + ABZR86_RS19210 0.25 -1.0 297,034 + ABZR86_RS19210 0.26 -0.6 297,034 + ABZR86_RS19210 0.26 +0.5 297,035 - ABZR86_RS19210 0.26 -0.5 297,064 + ABZR86_RS19210 0.28 +0.0 297,064 + ABZR86_RS19210 0.28 +0.4 297,064 + ABZR86_RS19210 0.28 -1.1 297,064 + ABZR86_RS19210 0.28 -1.5 297,065 - ABZR86_RS19210 0.28 -1.0 297,065 - ABZR86_RS19210 0.28 -0.4 297,065 - ABZR86_RS19210 0.28 +0.5 297,065 - ABZR86_RS19210 0.28 -0.7 297,073 + ABZR86_RS19210 0.29 -0.0 297,074 - ABZR86_RS19210 0.29 +0.6 297,128 - ABZR86_RS19210 0.33 -0.3 297,476 - ABZR86_RS19210 0.61 -2.6 297,476 - ABZR86_RS19210 0.61 -0.6 297,484 + ABZR86_RS19210 0.62 -1.1 297,484 + ABZR86_RS19210 0.62 -1.7 297,484 + ABZR86_RS19210 0.62 -0.5 297,485 - ABZR86_RS19210 0.62 -1.1 297,550 + ABZR86_RS19210 0.67 -1.1 297,550 + ABZR86_RS19210 0.67 -1.0 297,550 + ABZR86_RS19210 0.67 -0.1 297,551 - ABZR86_RS19210 0.67 -0.8 297,551 - ABZR86_RS19210 0.67 +1.8 297,551 - ABZR86_RS19210 0.67 -2.4 297,551 - ABZR86_RS19210 0.67 -0.8 297,551 - ABZR86_RS19210 0.67 +0.3 297,551 - ABZR86_RS19210 0.67 -0.6 297,551 - ABZR86_RS19210 0.67 +0.8 297,682 + ABZR86_RS19210 0.77 -2.1 297,682 + ABZR86_RS19210 0.77 -0.1 297,683 - ABZR86_RS19210 0.77 +0.7 297,796 + ABZR86_RS19210 0.86 +0.2 297,958 + +0.1 297,958 + +0.8 297,959 - +0.7 298,001 + +0.7 298,001 + +0.9 298,053 + -1.6 298,054 - -1.3 298,070 + -0.6 298,116 + +0.6 298,116 + -1.5 298,157 + -0.5 298,158 - +0.1 298,158 - -0.3 298,271 + ABZR86_RS19215 0.15 -0.2 298,271 + ABZR86_RS19215 0.15 -0.3 298,271 + ABZR86_RS19215 0.15 -0.6 298,272 - ABZR86_RS19215 0.16 -2.2 298,272 - ABZR86_RS19215 0.16 -2.1 298,272 - ABZR86_RS19215 0.16 -0.2 298,307 + ABZR86_RS19215 0.20 -1.4 298,308 - ABZR86_RS19215 0.20 -0.7 298,308 - ABZR86_RS19215 0.20 -0.4 298,308 - ABZR86_RS19215 0.20 -1.1 298,355 + ABZR86_RS19215 0.27 -0.3 298,356 - ABZR86_RS19215 0.27 +0.4 298,356 - ABZR86_RS19215 0.27 -0.5 298,604 + ABZR86_RS19215 0.60 -0.7 298,604 + ABZR86_RS19215 0.60 -0.7 298,605 - ABZR86_RS19215 0.60 -1.1 298,785 + ABZR86_RS19215 0.84 -0.4 298,785 + ABZR86_RS19215 0.84 -0.7 298,836 - -0.6 298,836 - -1.1 298,836 - +0.4 298,908 + -0.9 298,909 - -0.6
Or see this region's nucleotide sequence