Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS18000 and ABZR86_RS18005 are separated by 47 nucleotides ABZR86_RS18005 and ABZR86_RS18010 are separated by 118 nucleotides ABZR86_RS18010 and ABZR86_RS18015 are separated by 129 nucleotides ABZR86_RS18015 and ABZR86_RS18020 are separated by 104 nucleotides
ABZR86_RS18000: ABZR86_RS18000 - putative quinol monooxygenase, at 15,349 to 15,651
_RS18000
ABZR86_RS18005: ABZR86_RS18005 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 15,699 to 16,328
_RS18005
ABZR86_RS18010: ABZR86_RS18010 - aldo/keto reductase, at 16,447 to 17,259
_RS18010
ABZR86_RS18015: ABZR86_RS18015 - TetR/AcrR family transcriptional regulator, at 17,389 to 18,003
_RS18015
ABZR86_RS18020: ABZR86_RS18020 - MBL fold metallo-hydrolase, at 18,108 to 19,235
_RS18020
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 15.663 kb on + strand at 15.682 kb on - strand at 16.171 kb on - strand, within ABZR86_RS18005 at 16.178 kb on + strand, within ABZR86_RS18005 at 16.178 kb on + strand, within ABZR86_RS18005 at 16.179 kb on - strand, within ABZR86_RS18005 at 16.185 kb on + strand, within ABZR86_RS18005 at 16.186 kb on - strand, within ABZR86_RS18005 at 16.186 kb on - strand, within ABZR86_RS18005 at 16.307 kb on + strand at 16.435 kb on + strand at 16.436 kb on - strand at 16.508 kb on - strand at 16.602 kb on + strand, within ABZR86_RS18010 at 16.615 kb on + strand, within ABZR86_RS18010 at 16.615 kb on + strand, within ABZR86_RS18010 at 16.616 kb on - strand, within ABZR86_RS18010 at 16.786 kb on - strand, within ABZR86_RS18010 at 16.786 kb on - strand, within ABZR86_RS18010 at 16.786 kb on - strand, within ABZR86_RS18010 at 16.811 kb on + strand, within ABZR86_RS18010 at 16.811 kb on + strand, within ABZR86_RS18010 at 16.852 kb on + strand, within ABZR86_RS18010 at 16.871 kb on - strand, within ABZR86_RS18010 at 16.885 kb on + strand, within ABZR86_RS18010 at 17.322 kb on - strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.330 kb on + strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.331 kb on - strand at 17.336 kb on + strand at 17.336 kb on + strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.337 kb on - strand at 17.396 kb on + strand at 17.413 kb on - strand at 17.765 kb on + strand, within ABZR86_RS18015 at 17.766 kb on - strand, within ABZR86_RS18015 at 17.852 kb on + strand, within ABZR86_RS18015 at 17.853 kb on - strand, within ABZR86_RS18015 at 18.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 15,663 + +0.9 15,682 - +0.6 16,171 - ABZR86_RS18005 0.75 -1.5 16,178 + ABZR86_RS18005 0.76 -0.1 16,178 + ABZR86_RS18005 0.76 +0.3 16,179 - ABZR86_RS18005 0.76 -1.0 16,185 + ABZR86_RS18005 0.77 -0.9 16,186 - ABZR86_RS18005 0.77 +0.6 16,186 - ABZR86_RS18005 0.77 +0.1 16,307 + -0.4 16,435 + +2.3 16,436 - +0.7 16,508 - -0.4 16,602 + ABZR86_RS18010 0.19 +0.1 16,615 + ABZR86_RS18010 0.21 +0.3 16,615 + ABZR86_RS18010 0.21 -0.1 16,616 - ABZR86_RS18010 0.21 -1.0 16,786 - ABZR86_RS18010 0.42 -0.2 16,786 - ABZR86_RS18010 0.42 +1.6 16,786 - ABZR86_RS18010 0.42 -0.1 16,811 + ABZR86_RS18010 0.45 +0.7 16,811 + ABZR86_RS18010 0.45 -1.8 16,852 + ABZR86_RS18010 0.50 +0.2 16,871 - ABZR86_RS18010 0.52 +0.6 16,885 + ABZR86_RS18010 0.54 -0.8 17,322 - -1.7 17,330 + -0.8 17,330 + +1.9 17,330 + +0.3 17,330 + -1.1 17,330 + +1.0 17,331 - +0.4 17,331 - -1.0 17,331 - -1.8 17,331 - +0.3 17,331 - -1.0 17,331 - -1.5 17,331 - +0.1 17,331 - -1.0 17,331 - +0.3 17,336 + -0.3 17,336 + +0.7 17,337 - +2.0 17,337 - +0.3 17,337 - +0.6 17,337 - +1.0 17,396 + -1.4 17,413 - +0.1 17,765 + ABZR86_RS18015 0.61 -0.5 17,766 - ABZR86_RS18015 0.61 +1.0 17,852 + ABZR86_RS18015 0.75 -0.0 17,853 - ABZR86_RS18015 0.75 +0.2 18,107 + -0.9
Or see this region's nucleotide sequence