Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS16555 and ABZR86_RS16560 are separated by 7 nucleotides ABZR86_RS16560 and ABZR86_RS16565 are separated by 20 nucleotides ABZR86_RS16565 and ABZR86_RS16570 are separated by 164 nucleotides
ABZR86_RS16555: ABZR86_RS16555 - DMT family transporter, at 3,739,807 to 3,740,736
_RS16555
ABZR86_RS16560: ABZR86_RS16560 - cupin domain-containing protein, at 3,740,744 to 3,741,040
_RS16560
ABZR86_RS16565: ABZR86_RS16565 - type II toxin-antitoxin system HicB family antitoxin, at 3,741,061 to 3,741,318
_RS16565
ABZR86_RS16570: ABZR86_RS16570 - glutathione S-transferase family protein, at 3,741,483 to 3,742,169
_RS16570
Position (kb)
3740
3741
3742 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3739.770 kb on - strand at 3739.846 kb on + strand at 3739.846 kb on + strand at 3739.846 kb on + strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.847 kb on - strand at 3739.866 kb on + strand at 3739.866 kb on + strand at 3739.866 kb on + strand at 3739.866 kb on + strand at 3739.867 kb on - strand at 3739.867 kb on - strand at 3739.867 kb on - strand at 3739.882 kb on + strand at 3740.056 kb on + strand, within ABZR86_RS16555 at 3740.057 kb on - strand, within ABZR86_RS16555 at 3740.057 kb on - strand, within ABZR86_RS16555 at 3740.285 kb on + strand, within ABZR86_RS16555 at 3740.285 kb on + strand, within ABZR86_RS16555 at 3740.285 kb on + strand, within ABZR86_RS16555 at 3740.286 kb on - strand, within ABZR86_RS16555 at 3740.286 kb on - strand, within ABZR86_RS16555 at 3740.494 kb on + strand, within ABZR86_RS16555 at 3740.494 kb on + strand, within ABZR86_RS16555 at 3740.494 kb on + strand, within ABZR86_RS16555 at 3740.495 kb on - strand, within ABZR86_RS16555 at 3740.554 kb on + strand, within ABZR86_RS16555 at 3740.555 kb on - strand, within ABZR86_RS16555 at 3740.555 kb on - strand, within ABZR86_RS16555 at 3740.555 kb on - strand, within ABZR86_RS16555 at 3740.861 kb on + strand, within ABZR86_RS16560 at 3740.966 kb on + strand, within ABZR86_RS16560 at 3740.966 kb on + strand, within ABZR86_RS16560 at 3740.966 kb on + strand, within ABZR86_RS16560 at 3740.966 kb on + strand, within ABZR86_RS16560 at 3740.967 kb on - strand, within ABZR86_RS16560 at 3740.967 kb on - strand, within ABZR86_RS16560 at 3741.016 kb on + strand at 3741.016 kb on + strand at 3741.062 kb on + strand at 3741.062 kb on + strand at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.171 kb on + strand, within ABZR86_RS16565 at 3741.172 kb on - strand, within ABZR86_RS16565 at 3741.172 kb on - strand, within ABZR86_RS16565 at 3741.172 kb on - strand, within ABZR86_RS16565 at 3741.172 kb on - strand, within ABZR86_RS16565 at 3741.172 kb on - strand, within ABZR86_RS16565 at 3741.174 kb on - strand, within ABZR86_RS16565 at 3741.174 kb on - strand, within ABZR86_RS16565 at 3741.174 kb on - strand, within ABZR86_RS16565 at 3741.270 kb on + strand, within ABZR86_RS16565 at 3741.270 kb on + strand, within ABZR86_RS16565 at 3741.270 kb on + strand, within ABZR86_RS16565 at 3741.271 kb on - strand, within ABZR86_RS16565 at 3741.271 kb on - strand, within ABZR86_RS16565 at 3741.278 kb on + strand, within ABZR86_RS16565 at 3741.278 kb on + strand, within ABZR86_RS16565 at 3741.279 kb on - strand, within ABZR86_RS16565 at 3741.279 kb on - strand, within ABZR86_RS16565 at 3741.353 kb on + strand at 3741.353 kb on + strand at 3741.353 kb on + strand at 3741.354 kb on - strand at 3741.354 kb on - strand at 3741.354 kb on - strand at 3741.410 kb on + strand at 3741.486 kb on + strand at 3741.486 kb on + strand at 3741.486 kb on + strand at 3741.486 kb on + strand at 3741.487 kb on - strand at 3741.487 kb on - strand at 3741.487 kb on - strand at 3741.496 kb on - strand at 3741.513 kb on + strand at 3741.514 kb on - strand at 3741.514 kb on - strand at 3741.602 kb on + strand, within ABZR86_RS16570 at 3741.602 kb on + strand, within ABZR86_RS16570 at 3741.603 kb on - strand, within ABZR86_RS16570 at 3741.603 kb on - strand, within ABZR86_RS16570 at 3741.603 kb on - strand, within ABZR86_RS16570 at 3741.603 kb on - strand, within ABZR86_RS16570 at 3741.603 kb on - strand, within ABZR86_RS16570 at 3741.699 kb on + strand, within ABZR86_RS16570 at 3741.700 kb on - strand, within ABZR86_RS16570 at 3741.966 kb on + strand, within ABZR86_RS16570 at 3741.982 kb on - strand, within ABZR86_RS16570 at 3741.996 kb on + strand, within ABZR86_RS16570 at 3741.996 kb on + strand, within ABZR86_RS16570 at 3741.997 kb on - strand, within ABZR86_RS16570 at 3741.997 kb on - strand, within ABZR86_RS16570 at 3741.997 kb on - strand, within ABZR86_RS16570
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 3,739,770 - +0.4 3,739,846 + -0.5 3,739,846 + -0.1 3,739,846 + -0.1 3,739,847 - +0.4 3,739,847 - -0.0 3,739,847 - +1.7 3,739,847 - +0.4 3,739,847 - +0.3 3,739,847 - -2.4 3,739,847 - +0.3 3,739,847 - +0.2 3,739,847 - +1.2 3,739,866 + -0.5 3,739,866 + -0.6 3,739,866 + -0.6 3,739,866 + -0.7 3,739,867 - +1.2 3,739,867 - -0.9 3,739,867 - +1.9 3,739,882 + -1.7 3,740,056 + ABZR86_RS16555 0.27 -0.4 3,740,057 - ABZR86_RS16555 0.27 +0.4 3,740,057 - ABZR86_RS16555 0.27 -0.3 3,740,285 + ABZR86_RS16555 0.51 -0.4 3,740,285 + ABZR86_RS16555 0.51 -0.4 3,740,285 + ABZR86_RS16555 0.51 -0.8 3,740,286 - ABZR86_RS16555 0.52 +0.2 3,740,286 - ABZR86_RS16555 0.52 -0.9 3,740,494 + ABZR86_RS16555 0.74 -0.1 3,740,494 + ABZR86_RS16555 0.74 +1.0 3,740,494 + ABZR86_RS16555 0.74 -0.3 3,740,495 - ABZR86_RS16555 0.74 -0.2 3,740,554 + ABZR86_RS16555 0.80 -0.3 3,740,555 - ABZR86_RS16555 0.80 -0.7 3,740,555 - ABZR86_RS16555 0.80 -0.0 3,740,555 - ABZR86_RS16555 0.80 +0.9 3,740,861 + ABZR86_RS16560 0.39 -0.2 3,740,966 + ABZR86_RS16560 0.75 +1.2 3,740,966 + ABZR86_RS16560 0.75 -0.3 3,740,966 + ABZR86_RS16560 0.75 +0.9 3,740,966 + ABZR86_RS16560 0.75 -0.3 3,740,967 - ABZR86_RS16560 0.75 -1.5 3,740,967 - ABZR86_RS16560 0.75 -0.7 3,741,016 + +0.7 3,741,016 + +1.1 3,741,062 + +0.2 3,741,062 + -0.1 3,741,171 + ABZR86_RS16565 0.43 +0.4 3,741,171 + ABZR86_RS16565 0.43 -0.1 3,741,171 + ABZR86_RS16565 0.43 +0.2 3,741,171 + ABZR86_RS16565 0.43 -0.6 3,741,171 + ABZR86_RS16565 0.43 -0.3 3,741,171 + ABZR86_RS16565 0.43 -1.6 3,741,171 + ABZR86_RS16565 0.43 +0.1 3,741,171 + ABZR86_RS16565 0.43 -1.2 3,741,172 - ABZR86_RS16565 0.43 -0.6 3,741,172 - ABZR86_RS16565 0.43 -0.7 3,741,172 - ABZR86_RS16565 0.43 +0.7 3,741,172 - ABZR86_RS16565 0.43 -0.0 3,741,172 - ABZR86_RS16565 0.43 -0.1 3,741,174 - ABZR86_RS16565 0.44 +0.3 3,741,174 - ABZR86_RS16565 0.44 +0.1 3,741,174 - ABZR86_RS16565 0.44 -1.2 3,741,270 + ABZR86_RS16565 0.81 -0.2 3,741,270 + ABZR86_RS16565 0.81 +0.6 3,741,270 + ABZR86_RS16565 0.81 +1.0 3,741,271 - ABZR86_RS16565 0.81 -1.3 3,741,271 - ABZR86_RS16565 0.81 -0.8 3,741,278 + ABZR86_RS16565 0.84 -0.5 3,741,278 + ABZR86_RS16565 0.84 -0.5 3,741,279 - ABZR86_RS16565 0.84 -0.4 3,741,279 - ABZR86_RS16565 0.84 -0.2 3,741,353 + +0.6 3,741,353 + -0.2 3,741,353 + +2.0 3,741,354 - -0.0 3,741,354 - +0.6 3,741,354 - -0.1 3,741,410 + -0.6 3,741,486 + +0.2 3,741,486 + +1.9 3,741,486 + -0.7 3,741,486 + +0.7 3,741,487 - -0.0 3,741,487 - -0.4 3,741,487 - -0.3 3,741,496 - +1.1 3,741,513 + +0.3 3,741,514 - +1.3 3,741,514 - +0.5 3,741,602 + ABZR86_RS16570 0.17 +1.9 3,741,602 + ABZR86_RS16570 0.17 -0.3 3,741,603 - ABZR86_RS16570 0.17 +0.4 3,741,603 - ABZR86_RS16570 0.17 -0.3 3,741,603 - ABZR86_RS16570 0.17 -1.4 3,741,603 - ABZR86_RS16570 0.17 -1.6 3,741,603 - ABZR86_RS16570 0.17 +0.9 3,741,699 + ABZR86_RS16570 0.31 -2.0 3,741,700 - ABZR86_RS16570 0.32 -0.1 3,741,966 + ABZR86_RS16570 0.70 -0.2 3,741,982 - ABZR86_RS16570 0.73 +0.8 3,741,996 + ABZR86_RS16570 0.75 +0.9 3,741,996 + ABZR86_RS16570 0.75 +0.5 3,741,997 - ABZR86_RS16570 0.75 +0.2 3,741,997 - ABZR86_RS16570 0.75 +0.1 3,741,997 - ABZR86_RS16570 0.75 +0.1
Or see this region's nucleotide sequence