Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS07925 and ABZR86_RS07930 overlap by 32 nucleotides ABZR86_RS07930 and ABZR86_RS07935 are separated by 7 nucleotides ABZR86_RS07935 and ABZR86_RS07940 are separated by 16 nucleotides ABZR86_RS07940 and ABZR86_RS07945 are separated by 168 nucleotides
ABZR86_RS07925: ABZR86_RS07925 - glycosyltransferase family 1 protein, at 1,760,213 to 1,761,373
_RS07925
ABZR86_RS07930: ABZR86_RS07930 - phosphatase PAP2 family protein, at 1,761,342 to 1,761,914
_RS07930
ABZR86_RS07935: ABZR86_RS07935 - 4'-phosphopantetheinyl transferase superfamily protein, at 1,761,922 to 1,762,608
_RS07935
ABZR86_RS07940: ABZR86_RS07940 - TetR/AcrR family transcriptional regulator, at 1,762,625 to 1,763,428
_RS07940
ABZR86_RS07945: ABZR86_RS07945 - hypothetical protein, at 1,763,597 to 1,764,511
_RS07945
Position (kb)
1761
1762
1763 Strain fitness (log2 ratio)
-1
0
1 at 1761.017 kb on + strand, within ABZR86_RS07925 at 1761.034 kb on - strand, within ABZR86_RS07925 at 1761.034 kb on - strand, within ABZR86_RS07925 at 1761.400 kb on + strand, within ABZR86_RS07930 at 1761.748 kb on - strand, within ABZR86_RS07930 at 1762.015 kb on + strand, within ABZR86_RS07935 at 1762.016 kb on - strand, within ABZR86_RS07935 at 1762.119 kb on - strand, within ABZR86_RS07935 at 1762.454 kb on + strand, within ABZR86_RS07935 at 1762.455 kb on - strand, within ABZR86_RS07935 at 1763.062 kb on - strand, within ABZR86_RS07940 at 1763.148 kb on - strand, within ABZR86_RS07940 at 1763.284 kb on - strand, within ABZR86_RS07940 at 1763.466 kb on - strand at 1763.521 kb on + strand at 1763.521 kb on + strand at 1763.522 kb on - strand at 1763.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 1,761,017 + ABZR86_RS07925 0.69 -0.4 1,761,034 - ABZR86_RS07925 0.71 -0.7 1,761,034 - ABZR86_RS07925 0.71 -0.1 1,761,400 + ABZR86_RS07930 0.10 +0.1 1,761,748 - ABZR86_RS07930 0.71 -0.3 1,762,015 + ABZR86_RS07935 0.14 -0.3 1,762,016 - ABZR86_RS07935 0.14 -1.0 1,762,119 - ABZR86_RS07935 0.29 -0.4 1,762,454 + ABZR86_RS07935 0.77 +0.1 1,762,455 - ABZR86_RS07935 0.78 -0.0 1,763,062 - ABZR86_RS07940 0.54 +0.1 1,763,148 - ABZR86_RS07940 0.65 +0.4 1,763,284 - ABZR86_RS07940 0.82 +0.5 1,763,466 - +0.1 1,763,521 + +0.0 1,763,521 + +0.2 1,763,522 - -0.5 1,763,599 - +0.0
Or see this region's nucleotide sequence