Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05200 and ABZR86_RS05205 are separated by 9 nucleotides ABZR86_RS05205 and ABZR86_RS05210 are separated by 4 nucleotides ABZR86_RS05210 and ABZR86_RS05215 are separated by 175 nucleotides ABZR86_RS05215 and ABZR86_RS05220 are separated by 34 nucleotides ABZR86_RS05220 and ABZR86_RS05225 are separated by 3 nucleotides ABZR86_RS05225 and ABZR86_RS05230 are separated by 50 nucleotides
ABZR86_RS05200: ABZR86_RS05200 - type II toxin-antitoxin system RelE/ParE family toxin, at 1,176,430 to 1,176,708
_RS05200
ABZR86_RS05205: ABZR86_RS05205 - HigA family addiction module antitoxin, at 1,176,718 to 1,177,002
_RS05205
ABZR86_RS05210: ABZR86_RS05210 - hypothetical protein, at 1,177,007 to 1,177,495
_RS05210
ABZR86_RS05215: ABZR86_RS05215 - O-acetyl-ADP-ribose deacetylase, at 1,177,671 to 1,178,201
_RS05215
ABZR86_RS05220: ABZR86_RS05220 - hypothetical protein, at 1,178,236 to 1,178,544
_RS05220
ABZR86_RS05225: ABZR86_RS05225 - hypothetical protein, at 1,178,548 to 1,178,754
_RS05225
ABZR86_RS05230: ABZR86_RS05230 - TetR family transcriptional regulator, at 1,178,805 to 1,179,437
_RS05230
Position (kb)
1177
1178
1179 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1176.691 kb on + strand at 1176.692 kb on - strand at 1176.713 kb on + strand at 1176.713 kb on + strand at 1176.713 kb on + strand at 1176.713 kb on + strand at 1176.714 kb on - strand at 1176.714 kb on - strand at 1176.714 kb on - strand at 1176.733 kb on - strand at 1176.756 kb on - strand, within ABZR86_RS05205 at 1176.759 kb on - strand, within ABZR86_RS05205 at 1176.759 kb on - strand, within ABZR86_RS05205 at 1176.766 kb on + strand, within ABZR86_RS05205 at 1176.767 kb on - strand, within ABZR86_RS05205 at 1176.767 kb on - strand, within ABZR86_RS05205 at 1176.767 kb on - strand, within ABZR86_RS05205 at 1177.042 kb on + strand at 1177.043 kb on - strand at 1177.054 kb on + strand at 1177.183 kb on - strand, within ABZR86_RS05210 at 1177.320 kb on + strand, within ABZR86_RS05210 at 1177.330 kb on + strand, within ABZR86_RS05210 at 1177.401 kb on - strand, within ABZR86_RS05210 at 1177.459 kb on + strand at 1177.460 kb on - strand at 1177.621 kb on + strand at 1177.622 kb on - strand at 1177.622 kb on - strand at 1177.622 kb on - strand at 1177.622 kb on - strand at 1177.631 kb on + strand at 1177.632 kb on - strand at 1177.633 kb on + strand at 1177.633 kb on + strand at 1177.633 kb on + strand at 1177.633 kb on + strand at 1177.633 kb on + strand at 1177.633 kb on + strand at 1177.634 kb on - strand at 1177.654 kb on - strand at 1177.660 kb on + strand at 1177.661 kb on - strand at 1177.661 kb on - strand at 1177.662 kb on + strand at 1177.662 kb on + strand at 1177.662 kb on + strand at 1177.662 kb on + strand at 1177.663 kb on - strand at 1177.715 kb on - strand at 1177.837 kb on + strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1178.212 kb on + strand at 1178.262 kb on - strand at 1178.262 kb on - strand at 1178.267 kb on - strand, within ABZR86_RS05220 at 1178.315 kb on - strand, within ABZR86_RS05220 at 1178.315 kb on - strand, within ABZR86_RS05220 at 1178.369 kb on - strand, within ABZR86_RS05220 at 1178.550 kb on - strand at 1178.584 kb on - strand, within ABZR86_RS05225 at 1178.790 kb on + strand at 1178.791 kb on - strand at 1178.792 kb on + strand at 1178.792 kb on + strand at 1178.793 kb on - strand at 1178.798 kb on + strand at 1178.799 kb on - strand at 1178.799 kb on - strand at 1178.799 kb on - strand at 1178.830 kb on + strand at 1178.830 kb on + strand at 1178.863 kb on + strand at 1178.863 kb on + strand at 1179.027 kb on + strand, within ABZR86_RS05230
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 1,176,691 + +0.9 1,176,692 - -0.2 1,176,713 + +0.0 1,176,713 + -0.0 1,176,713 + +1.3 1,176,713 + +0.2 1,176,714 - -0.1 1,176,714 - +0.3 1,176,714 - -0.1 1,176,733 - -0.0 1,176,756 - ABZR86_RS05205 0.13 -0.0 1,176,759 - ABZR86_RS05205 0.14 +0.1 1,176,759 - ABZR86_RS05205 0.14 -0.4 1,176,766 + ABZR86_RS05205 0.17 -0.0 1,176,767 - ABZR86_RS05205 0.17 +0.2 1,176,767 - ABZR86_RS05205 0.17 -0.7 1,176,767 - ABZR86_RS05205 0.17 +0.7 1,177,042 + -0.5 1,177,043 - -0.5 1,177,054 + -0.4 1,177,183 - ABZR86_RS05210 0.36 +0.1 1,177,320 + ABZR86_RS05210 0.64 -1.7 1,177,330 + ABZR86_RS05210 0.66 -0.8 1,177,401 - ABZR86_RS05210 0.81 -0.7 1,177,459 + +1.0 1,177,460 - +1.1 1,177,621 + -0.2 1,177,622 - +0.2 1,177,622 - +0.4 1,177,622 - -0.7 1,177,622 - -1.1 1,177,631 + -0.9 1,177,632 - -1.0 1,177,633 + +0.7 1,177,633 + +0.3 1,177,633 + +0.6 1,177,633 + +1.3 1,177,633 + -0.9 1,177,633 + -0.6 1,177,634 - +0.4 1,177,654 - -0.4 1,177,660 + -0.0 1,177,661 - -0.6 1,177,661 - -1.4 1,177,662 + +0.2 1,177,662 + +0.0 1,177,662 + +0.2 1,177,662 + +0.5 1,177,663 - +1.8 1,177,715 - +1.1 1,177,837 + ABZR86_RS05215 0.31 +0.1 1,177,838 - ABZR86_RS05215 0.31 -0.9 1,177,838 - ABZR86_RS05215 0.31 +0.1 1,177,838 - ABZR86_RS05215 0.31 +0.8 1,178,212 + +0.7 1,178,262 - -0.8 1,178,262 - -0.3 1,178,267 - ABZR86_RS05220 0.10 +1.7 1,178,315 - ABZR86_RS05220 0.26 -1.1 1,178,315 - ABZR86_RS05220 0.26 +0.7 1,178,369 - ABZR86_RS05220 0.43 -0.3 1,178,550 - +0.6 1,178,584 - ABZR86_RS05225 0.17 +0.4 1,178,790 + -0.5 1,178,791 - -0.2 1,178,792 + +0.9 1,178,792 + -1.8 1,178,793 - +0.4 1,178,798 + -0.9 1,178,799 - -0.4 1,178,799 - -0.3 1,178,799 - +0.6 1,178,830 + +0.7 1,178,830 + -0.1 1,178,863 + +0.2 1,178,863 + -0.4 1,179,027 + ABZR86_RS05230 0.35 -1.2
Or see this region's nucleotide sequence