Experiment: Spectinomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS03510 and ABZR86_RS03515 overlap by 4 nucleotides ABZR86_RS03515 and ABZR86_RS03520 are separated by 50 nucleotides ABZR86_RS03520 and ABZR86_RS03525 are separated by 107 nucleotides
ABZR86_RS03510: ABZR86_RS03510 - XdhC/CoxI family protein, at 750,691 to 751,785
_RS03510
ABZR86_RS03515: ABZR86_RS03515 - nucleotidyltransferase family protein, at 751,782 to 752,381
_RS03515
ABZR86_RS03520: ABZR86_RS03520 - (2Fe-2S)-binding protein, at 752,432 to 752,869
_RS03520
ABZR86_RS03525: ABZR86_RS03525 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 752,977 to 755,247
_RS03525
Position (kb)
752
753 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 751.522 kb on + strand, within ABZR86_RS03510 at 751.522 kb on + strand, within ABZR86_RS03510 at 751.522 kb on + strand, within ABZR86_RS03510 at 751.523 kb on - strand, within ABZR86_RS03510 at 751.523 kb on - strand, within ABZR86_RS03510 at 751.523 kb on - strand, within ABZR86_RS03510 at 751.540 kb on + strand, within ABZR86_RS03510 at 751.540 kb on + strand, within ABZR86_RS03510 at 751.541 kb on - strand, within ABZR86_RS03510 at 751.541 kb on - strand, within ABZR86_RS03510 at 751.541 kb on - strand, within ABZR86_RS03510 at 751.541 kb on - strand, within ABZR86_RS03510 at 751.573 kb on + strand, within ABZR86_RS03510 at 751.573 kb on + strand, within ABZR86_RS03510 at 751.573 kb on + strand, within ABZR86_RS03510 at 751.573 kb on + strand, within ABZR86_RS03510 at 751.574 kb on - strand, within ABZR86_RS03510 at 751.840 kb on - strand at 752.181 kb on + strand, within ABZR86_RS03515 at 752.299 kb on + strand, within ABZR86_RS03515 at 752.300 kb on - strand, within ABZR86_RS03515 at 752.300 kb on - strand, within ABZR86_RS03515 at 752.339 kb on + strand at 752.352 kb on + strand at 752.353 kb on - strand at 752.353 kb on - strand at 752.447 kb on - strand at 752.496 kb on + strand, within ABZR86_RS03520 at 752.496 kb on + strand, within ABZR86_RS03520 at 752.496 kb on + strand, within ABZR86_RS03520 at 752.726 kb on + strand, within ABZR86_RS03520 at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.840 kb on + strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 752.841 kb on - strand at 753.197 kb on + strand at 753.232 kb on + strand, within ABZR86_RS03525 at 753.232 kb on + strand, within ABZR86_RS03525 at 753.232 kb on + strand, within ABZR86_RS03525 at 753.232 kb on + strand, within ABZR86_RS03525 at 753.232 kb on + strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.233 kb on - strand, within ABZR86_RS03525 at 753.322 kb on + strand, within ABZR86_RS03525 at 753.626 kb on + strand, within ABZR86_RS03525 at 753.626 kb on + strand, within ABZR86_RS03525
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.04 mg/ml remove 751,522 + ABZR86_RS03510 0.76 -0.1 751,522 + ABZR86_RS03510 0.76 -0.1 751,522 + ABZR86_RS03510 0.76 -0.9 751,523 - ABZR86_RS03510 0.76 +0.5 751,523 - ABZR86_RS03510 0.76 +0.6 751,523 - ABZR86_RS03510 0.76 +2.7 751,540 + ABZR86_RS03510 0.78 +0.2 751,540 + ABZR86_RS03510 0.78 +0.4 751,541 - ABZR86_RS03510 0.78 -1.2 751,541 - ABZR86_RS03510 0.78 -0.7 751,541 - ABZR86_RS03510 0.78 +0.3 751,541 - ABZR86_RS03510 0.78 -0.0 751,573 + ABZR86_RS03510 0.81 +0.1 751,573 + ABZR86_RS03510 0.81 +0.7 751,573 + ABZR86_RS03510 0.81 +1.8 751,573 + ABZR86_RS03510 0.81 +0.3 751,574 - ABZR86_RS03510 0.81 -0.1 751,840 - -0.9 752,181 + ABZR86_RS03515 0.67 -0.3 752,299 + ABZR86_RS03515 0.86 +0.0 752,300 - ABZR86_RS03515 0.86 -0.6 752,300 - ABZR86_RS03515 0.86 +0.0 752,339 + +0.6 752,352 + +0.1 752,353 - -0.3 752,353 - -0.6 752,447 - -1.0 752,496 + ABZR86_RS03520 0.15 +0.0 752,496 + ABZR86_RS03520 0.15 -0.7 752,496 + ABZR86_RS03520 0.15 -0.9 752,726 + ABZR86_RS03520 0.67 -0.6 752,840 + +0.3 752,840 + -0.4 752,840 + +0.1 752,840 + +0.2 752,840 + -0.2 752,840 + +0.1 752,840 + +0.4 752,840 + -0.1 752,840 + +2.8 752,840 + +0.0 752,840 + +0.3 752,840 + +1.1 752,841 - -0.1 752,841 - +0.9 752,841 - +0.3 752,841 - +1.2 752,841 - +0.2 752,841 - -0.8 752,841 - +0.5 752,841 - +0.2 752,841 - -0.7 753,197 + +1.1 753,232 + ABZR86_RS03525 0.11 -0.7 753,232 + ABZR86_RS03525 0.11 -1.1 753,232 + ABZR86_RS03525 0.11 -1.9 753,232 + ABZR86_RS03525 0.11 +0.2 753,232 + ABZR86_RS03525 0.11 +0.9 753,233 - ABZR86_RS03525 0.11 +0.1 753,233 - ABZR86_RS03525 0.11 -0.8 753,233 - ABZR86_RS03525 0.11 +0.7 753,233 - ABZR86_RS03525 0.11 -0.9 753,233 - ABZR86_RS03525 0.11 +0.4 753,233 - ABZR86_RS03525 0.11 +0.2 753,233 - ABZR86_RS03525 0.11 +1.1 753,322 + ABZR86_RS03525 0.15 -0.1 753,626 + ABZR86_RS03525 0.29 +0.3 753,626 + ABZR86_RS03525 0.29 +0.3
Or see this region's nucleotide sequence