Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2139 and SMa2141 are separated by 93 nucleotides SMa2141 and SMa2143 overlap by 4 nucleotides SMa2143 and SMa2145 are separated by 55 nucleotides
SMa2139: SMa2139 - SgaA serine-glyoxylate aminotransferase (SGAT), at 1,208,866 to 1,210,071
SMa2139
SMa2141: SMa2141 - formyltetrahydrofolate deformylase, at 1,210,165 to 1,211,025
SMa2141
SMa2143: SMa2143 - 5,10-methylenetetrahydrofolate reductase, at 1,211,022 to 1,211,975
SMa2143
SMa2145: SMa2145 - glycine cleavage system protein T, at 1,212,031 to 1,213,287
SMa2145
Position (kb)
1211
1212 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1210.059 kb on + strand at 1210.355 kb on + strand, within SMa2141 at 1210.355 kb on + strand, within SMa2141 at 1210.506 kb on - strand, within SMa2141 at 1210.608 kb on - strand, within SMa2141 at 1210.905 kb on - strand, within SMa2141 at 1210.934 kb on - strand, within SMa2141 at 1211.132 kb on + strand, within SMa2143 at 1211.133 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.278 kb on - strand, within SMa2143 at 1211.435 kb on + strand, within SMa2143 at 1211.517 kb on - strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.648 kb on - strand, within SMa2143 at 1211.712 kb on - strand, within SMa2143 at 1211.848 kb on - strand, within SMa2143 at 1211.886 kb on - strand at 1212.029 kb on - strand at 1212.250 kb on + strand, within SMa2145 at 1212.258 kb on - strand, within SMa2145 at 1212.362 kb on + strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.513 kb on - strand, within SMa2145 at 1212.513 kb on - strand, within SMa2145 at 1212.544 kb on + strand, within SMa2145 at 1212.545 kb on - strand, within SMa2145 at 1212.589 kb on - strand, within SMa2145 at 1212.803 kb on - strand, within SMa2145 at 1212.818 kb on + strand, within SMa2145 at 1212.819 kb on - strand, within SMa2145 at 1212.909 kb on + strand, within SMa2145 at 1212.910 kb on - strand, within SMa2145 at 1212.910 kb on - strand, within SMa2145 at 1212.951 kb on - strand, within SMa2145
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 1,210,059 + +2.2 1,210,355 + SMa2141 0.22 +0.3 1,210,355 + SMa2141 0.22 +0.7 1,210,506 - SMa2141 0.40 +0.0 1,210,608 - SMa2141 0.51 +1.1 1,210,905 - SMa2141 0.86 +1.2 1,210,934 - SMa2141 0.89 -0.7 1,211,132 + SMa2143 0.12 -3.0 1,211,133 - SMa2143 0.12 +0.5 1,211,221 - SMa2143 0.21 +1.3 1,211,221 - SMa2143 0.21 +1.7 1,211,278 - SMa2143 0.27 -0.3 1,211,435 + SMa2143 0.43 -0.9 1,211,517 - SMa2143 0.52 +0.7 1,211,647 + SMa2143 0.66 -2.7 1,211,647 + SMa2143 0.66 -0.0 1,211,648 - SMa2143 0.66 +1.3 1,211,712 - SMa2143 0.72 -1.6 1,211,848 - SMa2143 0.87 +2.2 1,211,886 - +0.8 1,212,029 - +0.1 1,212,250 + SMa2145 0.17 -0.4 1,212,258 - SMa2145 0.18 -1.6 1,212,362 + SMa2145 0.26 +0.7 1,212,511 - SMa2145 0.38 -0.3 1,212,511 - SMa2145 0.38 +3.1 1,212,511 - SMa2145 0.38 -1.0 1,212,513 - SMa2145 0.38 -1.5 1,212,513 - SMa2145 0.38 +1.3 1,212,544 + SMa2145 0.41 -0.7 1,212,545 - SMa2145 0.41 +0.6 1,212,589 - SMa2145 0.44 +0.7 1,212,803 - SMa2145 0.61 -2.9 1,212,818 + SMa2145 0.63 +0.2 1,212,819 - SMa2145 0.63 -3.3 1,212,909 + SMa2145 0.70 +0.4 1,212,910 - SMa2145 0.70 -0.8 1,212,910 - SMa2145 0.70 -3.0 1,212,951 - SMa2145 0.73 +0.3
Or see this region's nucleotide sequence