Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_769

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_768 and Pf1N1B4_769 are separated by 369 nucleotidesPf1N1B4_769 and Pf1N1B4_770 are separated by 324 nucleotidesPf1N1B4_770 and Pf1N1B4_771 overlap by 18 nucleotides Pf1N1B4_768 - Glycerol-3-phosphate regulon repressor, DeoR family, at 872,576 to 873,331 _768 Pf1N1B4_769 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 873,701 to 875,161 _769 Pf1N1B4_770 - hypothetical protein, at 875,486 to 875,626 _770 Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component (from data), at 875,609 to 876,523 _771 Position (kb) 873 874 875 876Strain fitness (log2 ratio) -2 -1 0 1 2 3at 873.329 kb on + strandat 873.398 kb on + strandat 873.398 kb on + strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.399 kb on - strandat 873.401 kb on - strandat 873.401 kb on - strandat 873.474 kb on - strandat 873.474 kb on - strandat 873.474 kb on - strandat 873.474 kb on - strandat 873.474 kb on - strandat 873.474 kb on - strandat 873.595 kb on + strandat 873.597 kb on + strandat 873.635 kb on + strandat 873.635 kb on + strandat 873.635 kb on + strandat 873.635 kb on + strandat 873.636 kb on - strandat 873.636 kb on - strandat 873.636 kb on - strandat 873.644 kb on + strandat 873.644 kb on + strandat 873.665 kb on + strandat 873.665 kb on + strandat 873.665 kb on + strandat 873.665 kb on + strandat 873.666 kb on - strandat 873.831 kb on - strandat 873.977 kb on + strand, within Pf1N1B4_769at 874.116 kb on + strand, within Pf1N1B4_769at 874.116 kb on + strand, within Pf1N1B4_769at 874.370 kb on + strand, within Pf1N1B4_769at 874.370 kb on + strand, within Pf1N1B4_769at 874.370 kb on + strand, within Pf1N1B4_769at 874.475 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.589 kb on + strand, within Pf1N1B4_769at 874.652 kb on + strand, within Pf1N1B4_769at 874.653 kb on - strand, within Pf1N1B4_769at 874.766 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.913 kb on + strand, within Pf1N1B4_769at 874.957 kb on + strand, within Pf1N1B4_769at 874.991 kb on + strand, within Pf1N1B4_769at 874.991 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.009 kb on + strand, within Pf1N1B4_769at 875.114 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.225 kb on + strandat 875.226 kb on - strandat 875.226 kb on - strandat 875.281 kb on + strandat 875.282 kb on - strandat 875.284 kb on + strandat 875.284 kb on + strandat 875.285 kb on - strandat 875.285 kb on - strandat 875.299 kb on + strandat 875.340 kb on - strandat 875.410 kb on - strandat 875.464 kb on - strandat 875.494 kb on + strandat 875.582 kb on + strand, within Pf1N1B4_770at 875.582 kb on + strand, within Pf1N1B4_770at 875.583 kb on - strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.584 kb on + strand, within Pf1N1B4_770at 875.762 kb on + strand, within Pf1N1B4_771at 875.795 kb on + strand, within Pf1N1B4_771at 875.871 kb on - strand, within Pf1N1B4_771at 875.871 kb on - strand, within Pf1N1B4_771at 876.035 kb on + strand, within Pf1N1B4_771at 876.036 kb on - strand, within Pf1N1B4_771at 876.036 kb on - strandat 876.036 kb on - strand, within Pf1N1B4_771

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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873,329 + -0.1
873,398 + +0.2
873,398 + +0.0
873,399 - -0.8
873,399 - -0.1
873,399 - +0.9
873,399 - +0.6
873,399 - +0.3
873,399 - +3.0
873,399 - -1.5
873,399 - -1.0
873,401 - -2.4
873,401 - -0.2
873,474 - -0.3
873,474 - +1.0
873,474 - -0.0
873,474 - -0.3
873,474 - +0.3
873,474 - +0.0
873,595 + -1.8
873,597 + +1.0
873,635 + -2.1
873,635 + -1.9
873,635 + -1.1
873,635 + -1.0
873,636 - -1.4
873,636 - -1.0
873,636 - -2.1
873,644 + -1.7
873,644 + -1.4
873,665 + -0.3
873,665 + -1.3
873,665 + +0.6
873,665 + -2.1
873,666 - -1.1
873,831 - -0.2
873,977 + Pf1N1B4_769 0.19 -1.8
874,116 + Pf1N1B4_769 0.28 -1.5
874,116 + Pf1N1B4_769 0.28 -1.5
874,370 + Pf1N1B4_769 0.46 -0.5
874,370 + Pf1N1B4_769 0.46 -0.2
874,370 + Pf1N1B4_769 0.46 -0.4
874,475 + Pf1N1B4_769 0.53 +0.0
874,589 + Pf1N1B4_769 0.61 -0.8
874,589 + Pf1N1B4_769 0.61 -1.3
874,589 + Pf1N1B4_769 0.61 -0.2
874,589 + Pf1N1B4_769 0.61 -0.5
874,652 + Pf1N1B4_769 0.65 -1.3
874,653 - Pf1N1B4_769 0.65 +1.6
874,766 + Pf1N1B4_769 0.73 -1.0
874,913 + Pf1N1B4_769 0.83 -1.8
874,913 + Pf1N1B4_769 0.83 -1.9
874,913 + Pf1N1B4_769 0.83 -1.0
874,913 + Pf1N1B4_769 0.83 -2.0
874,957 + Pf1N1B4_769 0.86 -1.0
874,991 + Pf1N1B4_769 0.88 -1.5
874,991 + Pf1N1B4_769 0.88 -1.5
875,009 + Pf1N1B4_769 0.90 -1.4
875,009 + Pf1N1B4_769 0.90 +0.0
875,009 + Pf1N1B4_769 0.90 -1.6
875,114 + -1.1
875,225 + +2.0
875,225 + -0.3
875,225 + +0.8
875,225 + -2.1
875,226 - +0.0
875,226 - +0.0
875,281 + -2.1
875,282 - -0.7
875,284 + -0.5
875,284 + -0.5
875,285 - +1.0
875,285 - -1.8
875,299 + -0.1
875,340 - -0.5
875,410 - +0.2
875,464 - -2.3
875,494 + -1.0
875,582 + Pf1N1B4_770 0.68 -0.8
875,582 + Pf1N1B4_770 0.68 -0.8
875,583 - Pf1N1B4_770 0.69 +0.6
875,584 + Pf1N1B4_770 0.70 -0.5
875,584 + Pf1N1B4_770 0.70 +1.9
875,584 + Pf1N1B4_770 0.70 -1.0
875,584 + Pf1N1B4_770 0.70 +0.0
875,762 + Pf1N1B4_771 0.17 -0.3
875,795 + Pf1N1B4_771 0.20 +0.4
875,871 - Pf1N1B4_771 0.29 +0.6
875,871 - Pf1N1B4_771 0.29 +0.7
876,035 + Pf1N1B4_771 0.47 +1.0
876,036 - Pf1N1B4_771 0.47 -1.5
876,036 - -0.5
876,036 - Pf1N1B4_771 0.47 -0.4

Or see this region's nucleotide sequence