Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_695

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_694 and Pf1N1B4_695 are separated by 218 nucleotidesPf1N1B4_695 and Pf1N1B4_696 overlap by 4 nucleotides Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314 _694 Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981 _695 Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615 _696 Position (kb) 793 794 795Strain fitness (log2 ratio) -2 -1 0 1 2at 792.539 kb on + strand, within Pf1N1B4_694at 792.540 kb on - strand, within Pf1N1B4_694at 792.581 kb on + strand, within Pf1N1B4_694at 792.581 kb on + strand, within Pf1N1B4_694at 792.582 kb on - strand, within Pf1N1B4_694at 792.666 kb on + strand, within Pf1N1B4_694at 792.818 kb on + strand, within Pf1N1B4_694at 792.818 kb on + strand, within Pf1N1B4_694at 792.818 kb on + strand, within Pf1N1B4_694at 792.818 kb on + strand, within Pf1N1B4_694at 792.819 kb on - strand, within Pf1N1B4_694at 792.819 kb on - strand, within Pf1N1B4_694at 792.855 kb on - strand, within Pf1N1B4_694at 793.025 kb on + strand, within Pf1N1B4_694at 793.109 kb on + strand, within Pf1N1B4_694at 793.110 kb on - strand, within Pf1N1B4_694at 793.110 kb on - strand, within Pf1N1B4_694at 793.337 kb on - strandat 793.337 kb on - strandat 793.337 kb on - strandat 793.340 kb on + strandat 793.340 kb on + strandat 793.340 kb on + strandat 793.341 kb on - strandat 793.341 kb on - strandat 793.801 kb on - strand, within Pf1N1B4_695at 793.914 kb on - strand, within Pf1N1B4_695at 794.196 kb on + strand, within Pf1N1B4_695at 794.450 kb on + strand, within Pf1N1B4_695at 794.457 kb on + strand, within Pf1N1B4_695at 794.641 kb on + strandat 794.642 kb on - strand, within Pf1N1B4_695at 794.645 kb on - strand, within Pf1N1B4_695at 794.645 kb on - strand, within Pf1N1B4_695at 794.950 kb on + strandat 794.950 kb on + strandat 794.950 kb on + strandat 794.951 kb on - strandat 794.951 kb on - strandat 794.951 kb on - strandat 794.966 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.141 kb on + strandat 795.142 kb on - strand, within Pf1N1B4_696at 795.142 kb on - strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.183 kb on + strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.184 kb on - strand, within Pf1N1B4_696at 795.202 kb on - strand, within Pf1N1B4_696at 795.453 kb on + strand, within Pf1N1B4_696at 795.701 kb on - strand, within Pf1N1B4_696

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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792,539 + Pf1N1B4_694 0.30 -0.4
792,540 - Pf1N1B4_694 0.30 -0.8
792,581 + Pf1N1B4_694 0.34 +1.0
792,581 + Pf1N1B4_694 0.34 +0.5
792,582 - Pf1N1B4_694 0.34 -0.3
792,666 + Pf1N1B4_694 0.41 +1.4
792,818 + Pf1N1B4_694 0.55 -1.6
792,818 + Pf1N1B4_694 0.55 +1.6
792,818 + Pf1N1B4_694 0.55 -0.2
792,818 + Pf1N1B4_694 0.55 +0.4
792,819 - Pf1N1B4_694 0.55 -0.6
792,819 - Pf1N1B4_694 0.55 -0.9
792,855 - Pf1N1B4_694 0.58 +1.3
793,025 + Pf1N1B4_694 0.74 +0.5
793,109 + Pf1N1B4_694 0.81 +0.5
793,110 - Pf1N1B4_694 0.81 -1.2
793,110 - Pf1N1B4_694 0.81 -0.6
793,337 - +0.7
793,337 - +0.9
793,337 - -0.6
793,340 + +0.1
793,340 + -0.1
793,340 + +0.2
793,341 - -0.4
793,341 - +0.2
793,801 - Pf1N1B4_695 0.18 +0.3
793,914 - Pf1N1B4_695 0.26 -1.5
794,196 + Pf1N1B4_695 0.46 +0.4
794,450 + Pf1N1B4_695 0.63 -1.6
794,457 + Pf1N1B4_695 0.64 -0.6
794,641 + -0.6
794,642 - Pf1N1B4_695 0.77 -0.0
794,645 - Pf1N1B4_695 0.77 -0.6
794,645 - Pf1N1B4_695 0.77 -0.9
794,950 + -0.7
794,950 + +0.6
794,950 + -0.9
794,951 - -0.5
794,951 - +2.0
794,951 - +0.7
794,966 + +0.8
795,141 + -0.6
795,141 + -2.5
795,141 + -2.6
795,141 + +0.6
795,141 + +0.4
795,141 + -0.6
795,141 + +0.2
795,142 - Pf1N1B4_696 0.10 -2.6
795,142 - Pf1N1B4_696 0.10 -0.4
795,183 + Pf1N1B4_696 0.13 -0.6
795,183 + Pf1N1B4_696 0.13 -0.0
795,183 + Pf1N1B4_696 0.13 -0.1
795,183 + Pf1N1B4_696 0.13 +1.1
795,183 + Pf1N1B4_696 0.13 +2.0
795,183 + Pf1N1B4_696 0.13 -0.6
795,184 - Pf1N1B4_696 0.13 -0.8
795,184 - Pf1N1B4_696 0.13 -0.7
795,184 - Pf1N1B4_696 0.13 -0.1
795,184 - Pf1N1B4_696 0.13 -1.3
795,184 - Pf1N1B4_696 0.13 -0.6
795,184 - Pf1N1B4_696 0.13 +1.4
795,202 - Pf1N1B4_696 0.14 +0.7
795,453 + Pf1N1B4_696 0.29 +1.3
795,701 - Pf1N1B4_696 0.44 +0.3

Or see this region's nucleotide sequence