Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_62 and Pf1N1B4_63 are separated by 200 nucleotides Pf1N1B4_63 and Pf1N1B4_64 are separated by 109 nucleotides Pf1N1B4_64 and Pf1N1B4_65 are separated by 194 nucleotides
Pf1N1B4_62 - VacJ-like lipoprotein precursor, at 65,847 to 66,536
_62
Pf1N1B4_63 - Phosphonoacetaldehyde phosphonohydrolase-related protein, at 66,737 to 67,357
_63
Pf1N1B4_64 - Chromosome segregation ATPases, at 67,467 to 67,730
_64
Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755
_65
Position (kb) 67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.598 kb on - strand at 66.600 kb on - strand at 66.600 kb on - strand at 66.619 kb on - strand at 66.707 kb on + strand at 66.716 kb on + strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.737 kb on - strand at 66.786 kb on + strand at 66.786 kb on + strand at 66.787 kb on - strand at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.830 kb on - strand, within Pf1N1B4_63 at 66.991 kb on + strand at 66.992 kb on - strand at 66.992 kb on - strand, within Pf1N1B4_63 at 67.322 kb on - strand at 67.390 kb on - strand at 67.394 kb on + strand at 67.394 kb on + strand at 67.395 kb on - strand at 67.395 kb on - strand at 67.408 kb on - strand at 67.408 kb on - strand at 67.433 kb on + strand at 67.434 kb on - strand at 67.451 kb on + strand at 67.741 kb on + strand at 67.741 kb on + strand at 67.838 kb on + strand at 67.838 kb on + strand at 67.839 kb on - strand at 67.839 kb on - strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.923 kb on + strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.924 kb on - strand at 67.927 kb on - strand at 67.927 kb on - strand at 68.011 kb on - strand, within Pf1N1B4_65 at 68.011 kb on - strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.019 kb on + strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.020 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.122 kb on - strand, within Pf1N1B4_65 at 68.232 kb on - strand, within Pf1N1B4_65 at 68.245 kb on + strand, within Pf1N1B4_65 at 68.246 kb on - strand at 68.292 kb on + strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.293 kb on - strand, within Pf1N1B4_65 at 68.484 kb on + strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.485 kb on - strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand, within Pf1N1B4_65 at 68.619 kb on + strand at 68.620 kb on - strand, within Pf1N1B4_65 at 68.620 kb on - strand, within Pf1N1B4_65 at 68.698 kb on - strand at 68.698 kb on - strand at 68.709 kb on + strand at 68.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 66,598 - -0.4 66,598 - +0.6 66,598 - +0.9 66,598 - -0.5 66,598 - +1.8 66,598 - +2.3 66,600 - +0.8 66,600 - +0.3 66,619 - +2.8 66,707 + +0.1 66,716 + +0.8 66,717 - -2.0 66,717 - +1.3 66,717 - +0.8 66,717 - +1.8 66,719 + -0.8 66,720 - -0.7 66,720 - +1.8 66,720 - +1.3 66,720 - +1.5 66,720 - -0.4 66,737 - +0.2 66,786 + -0.9 66,786 + -0.8 66,787 - +0.8 66,830 - Pf1N1B4_63 0.15 +0.0 66,830 - Pf1N1B4_63 0.15 -0.4 66,830 - Pf1N1B4_63 0.15 -2.9 66,991 + +0.8 66,992 - -0.6 66,992 - Pf1N1B4_63 0.41 -0.5 67,322 - +1.2 67,390 - -1.6 67,394 + +0.8 67,394 + -0.6 67,395 - -2.2 67,395 - -0.2 67,408 - +0.3 67,408 - +0.8 67,433 + -0.8 67,434 - -0.2 67,451 + +0.2 67,741 + +0.2 67,741 + +0.5 67,838 + -0.8 67,838 + -0.3 67,839 - -0.3 67,839 - -0.8 67,923 + +0.1 67,923 + -0.8 67,923 + -0.6 67,924 - -1.2 67,924 - -0.3 67,924 - +1.0 67,924 - +0.4 67,924 - +0.3 67,924 - -0.8 67,924 - +0.4 67,924 - +0.8 67,927 - +0.8 67,927 - -0.9 68,011 - Pf1N1B4_65 0.10 +0.3 68,011 - Pf1N1B4_65 0.10 -0.0 68,019 + Pf1N1B4_65 0.11 -0.2 68,019 + Pf1N1B4_65 0.11 +2.1 68,019 + Pf1N1B4_65 0.11 +0.2 68,020 - Pf1N1B4_65 0.11 -0.2 68,020 - Pf1N1B4_65 0.11 +0.4 68,020 - Pf1N1B4_65 0.11 -0.7 68,020 - Pf1N1B4_65 0.11 -1.2 68,122 - Pf1N1B4_65 0.24 +0.4 68,122 - Pf1N1B4_65 0.24 -0.6 68,232 - Pf1N1B4_65 0.37 -1.5 68,245 + Pf1N1B4_65 0.39 -0.2 68,246 - +2.8 68,292 + Pf1N1B4_65 0.44 +0.2 68,293 - Pf1N1B4_65 0.44 +0.2 68,293 - Pf1N1B4_65 0.44 +0.3 68,293 - Pf1N1B4_65 0.44 -0.7 68,293 - Pf1N1B4_65 0.44 -0.9 68,293 - Pf1N1B4_65 0.44 -0.6 68,293 - Pf1N1B4_65 0.44 -0.1 68,484 + Pf1N1B4_65 0.67 -1.0 68,485 - Pf1N1B4_65 0.67 -0.4 68,485 - Pf1N1B4_65 0.67 -0.8 68,485 - Pf1N1B4_65 0.67 -1.3 68,619 + Pf1N1B4_65 0.84 +1.8 68,619 + Pf1N1B4_65 0.84 +0.2 68,619 + +1.8 68,620 - Pf1N1B4_65 0.84 +0.8 68,620 - Pf1N1B4_65 0.84 +1.3 68,698 - +2.2 68,698 - -0.6 68,709 + +0.4 68,710 - +0.2
Or see this region's nucleotide sequence