Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_626 and Pf1N1B4_627 overlap by 33 nucleotides Pf1N1B4_627 and Pf1N1B4_5174 are separated by 170 nucleotides Pf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides
Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489
_626
Pf1N1B4_627 - hypothetical protein, at 725,457 to 725,573
_627
Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816
_5174
Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560
_628
Position (kb) 725
726 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 724.610 kb on + strand, within Pf1N1B4_626 at 724.935 kb on - strand, within Pf1N1B4_626 at 724.936 kb on + strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand at 725.072 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626 at 725.341 kb on + strand, within Pf1N1B4_626 at 725.341 kb on + strand, within Pf1N1B4_626 at 725.342 kb on - strand, within Pf1N1B4_626 at 725.397 kb on - strand at 725.404 kb on + strand at 725.405 kb on - strand at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.887 kb on + strand at 725.887 kb on + strand at 725.955 kb on - strand, within Pf1N1B4_628 at 726.096 kb on + strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.446 kb on - strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 724,610 + Pf1N1B4_626 0.17 -0.9 724,935 - Pf1N1B4_626 0.48 +0.7 724,936 + Pf1N1B4_626 0.48 +2.6 724,937 - Pf1N1B4_626 0.48 +0.7 724,937 - Pf1N1B4_626 0.48 +0.7 724,937 - Pf1N1B4_626 0.48 +0.2 724,937 - Pf1N1B4_626 0.48 +1.0 724,937 - Pf1N1B4_626 0.48 -1.0 724,937 - Pf1N1B4_626 0.48 -1.9 724,937 - Pf1N1B4_626 0.48 +0.2 724,937 - Pf1N1B4_626 0.48 -0.8 724,937 - Pf1N1B4_626 0.48 +2.0 724,937 - Pf1N1B4_626 0.48 +1.0 724,937 - Pf1N1B4_626 0.48 -1.3 724,937 - Pf1N1B4_626 0.48 -0.0 725,072 - -1.0 725,072 - Pf1N1B4_626 0.61 +0.4 725,072 - Pf1N1B4_626 0.61 -1.0 725,084 + Pf1N1B4_626 0.62 -0.5 725,084 + Pf1N1B4_626 0.62 -2.5 725,085 - Pf1N1B4_626 0.62 -0.8 725,085 - Pf1N1B4_626 0.62 -0.2 725,341 + Pf1N1B4_626 0.86 +1.6 725,341 + Pf1N1B4_626 0.86 -2.1 725,342 - Pf1N1B4_626 0.86 +0.5 725,397 - +0.7 725,404 + -1.0 725,405 - -0.9 725,532 - Pf1N1B4_627 0.64 -1.0 725,532 - Pf1N1B4_627 0.64 +1.5 725,532 - Pf1N1B4_627 0.64 -1.1 725,532 - Pf1N1B4_627 0.64 -1.6 725,532 - Pf1N1B4_627 0.64 -0.7 725,532 - Pf1N1B4_627 0.64 +0.2 725,532 - Pf1N1B4_627 0.64 -1.7 725,532 - +1.0 725,576 - -0.7 725,576 - +3.3 725,576 - +0.4 725,715 - +2.6 725,715 - +1.0 725,715 - +0.2 725,715 - +1.0 725,887 + -1.0 725,887 + +1.0 725,955 - Pf1N1B4_628 0.11 +2.3 726,096 + Pf1N1B4_628 0.32 +0.5 726,097 - Pf1N1B4_628 0.32 +0.4 726,097 - Pf1N1B4_628 0.32 -0.0 726,176 - Pf1N1B4_628 0.43 -1.6 726,176 - Pf1N1B4_628 0.43 +0.2 726,274 + Pf1N1B4_628 0.58 -0.8 726,274 + Pf1N1B4_628 0.58 -0.8 726,274 + Pf1N1B4_628 0.58 -0.2 726,274 + Pf1N1B4_628 0.58 +0.1 726,275 - Pf1N1B4_628 0.58 +0.7 726,275 - Pf1N1B4_628 0.58 +1.2 726,275 - Pf1N1B4_628 0.58 +0.6 726,320 + Pf1N1B4_628 0.65 -0.6 726,320 + Pf1N1B4_628 0.65 -1.8 726,321 - Pf1N1B4_628 0.65 -0.4 726,321 - Pf1N1B4_628 0.65 -1.0 726,321 - Pf1N1B4_628 0.65 -1.3 726,321 - Pf1N1B4_628 0.65 +0.2 726,321 - Pf1N1B4_628 0.65 +0.1 726,321 - Pf1N1B4_628 0.65 +1.6 726,446 - Pf1N1B4_628 0.83 +1.0 726,463 + Pf1N1B4_628 0.86 -0.6 726,463 + Pf1N1B4_628 0.86 -0.2 726,463 + Pf1N1B4_628 0.86 -0.0 726,464 - Pf1N1B4_628 0.86 +0.3 726,464 - Pf1N1B4_628 0.86 +0.3
Or see this region's nucleotide sequence