Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6004 and Pf1N1B4_6005 overlap by 4 nucleotides
Pf1N1B4_6004 - tgtA5 cluster protein 1, at 459,335 to 460,456
_6004
Pf1N1B4_6005 - archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 5, at 460,453 to 461,751
_6005
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 458.339 kb on + strand at 458.346 kb on + strand at 458.484 kb on + strand at 458.857 kb on - strand at 458.963 kb on - strand at 459.245 kb on - strand at 459.322 kb on + strand at 459.370 kb on - strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.402 kb on - strand at 459.402 kb on - strand at 459.402 kb on - strand at 459.408 kb on + strand at 459.534 kb on - strand, within Pf1N1B4_6004 at 459.571 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 460.029 kb on + strand at 460.082 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.125 kb on - strand, within Pf1N1B4_6004 at 460.131 kb on + strand, within Pf1N1B4_6004 at 460.131 kb on + strand, within Pf1N1B4_6004 at 460.132 kb on - strand at 460.162 kb on + strand, within Pf1N1B4_6004 at 460.256 kb on + strand, within Pf1N1B4_6004 at 460.256 kb on + strand, within Pf1N1B4_6004 at 460.299 kb on + strand, within Pf1N1B4_6004 at 460.360 kb on + strand at 460.399 kb on + strand at 460.400 kb on - strand at 460.433 kb on + strand at 460.433 kb on + strand at 460.435 kb on + strand at 460.768 kb on + strand, within Pf1N1B4_6005 at 460.772 kb on + strand, within Pf1N1B4_6005 at 461.093 kb on + strand, within Pf1N1B4_6005
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 458,339 + +0.5 458,346 + +0.4 458,484 + -1.5 458,857 - +0.7 458,963 - +0.7 459,245 - -1.3 459,322 + -0.3 459,370 - -2.1 459,401 + +1.0 459,401 + +0.7 459,401 + -0.3 459,401 + -0.9 459,402 - +3.0 459,402 - -1.2 459,402 - -0.9 459,408 + -0.4 459,534 - Pf1N1B4_6004 0.18 -1.7 459,571 + Pf1N1B4_6004 0.21 -0.9 459,814 + Pf1N1B4_6004 0.43 -0.3 459,814 + Pf1N1B4_6004 0.43 -1.3 459,814 + Pf1N1B4_6004 0.43 -0.3 460,029 + -2.1 460,082 + Pf1N1B4_6004 0.67 -0.3 460,124 + Pf1N1B4_6004 0.70 -0.8 460,124 + Pf1N1B4_6004 0.70 -1.2 460,124 + Pf1N1B4_6004 0.70 -0.2 460,125 - Pf1N1B4_6004 0.70 -0.1 460,131 + Pf1N1B4_6004 0.71 -2.8 460,131 + Pf1N1B4_6004 0.71 +0.7 460,132 - -0.3 460,162 + Pf1N1B4_6004 0.74 -1.8 460,256 + Pf1N1B4_6004 0.82 +0.2 460,256 + Pf1N1B4_6004 0.82 -1.7 460,299 + Pf1N1B4_6004 0.86 +0.7 460,360 + -1.1 460,399 + +0.4 460,400 - +0.1 460,433 + -0.1 460,433 + +0.3 460,435 + +0.8 460,768 + Pf1N1B4_6005 0.24 -0.3 460,772 + Pf1N1B4_6005 0.25 +1.7 461,093 + Pf1N1B4_6005 0.49 +0.7
Or see this region's nucleotide sequence