Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5310 and Pf1N1B4_5311 are separated by 924 nucleotides Pf1N1B4_5311 and Pf1N1B4_5312 are separated by 242 nucleotides Pf1N1B4_5312 and Pf1N1B4_5313 are separated by 40 nucleotides
Pf1N1B4_5310 - hypothetical protein, at 60,819 to 61,040
_5310
Pf1N1B4_5311 - hypothetical protein, at 61,965 to 62,252
_5311
Pf1N1B4_5312 - Putative activity regulator of membrane protease YbbK, at 62,495 to 62,932
_5312
Pf1N1B4_5313 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 62,973 to 63,839
_5313
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 61.074 kb on + strand at 61.075 kb on - strand at 61.098 kb on - strand at 61.222 kb on - strand at 61.421 kb on - strand at 61.421 kb on - strand at 61.448 kb on - strand at 61.464 kb on + strand at 61.496 kb on + strand at 61.534 kb on + strand at 61.605 kb on - strand at 61.675 kb on + strand at 61.675 kb on + strand at 61.676 kb on - strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.680 kb on - strand at 61.714 kb on + strand at 61.714 kb on + strand at 61.723 kb on + strand at 61.866 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.921 kb on - strand at 61.921 kb on - strand at 61.921 kb on - strand at 62.067 kb on + strand, within Pf1N1B4_5311 at 62.068 kb on - strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.329 kb on + strand at 62.330 kb on + strand at 62.330 kb on + strand at 62.331 kb on - strand at 62.372 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.430 kb on + strand at 62.456 kb on + strand at 62.456 kb on + strand at 62.457 kb on - strand at 62.614 kb on + strand, within Pf1N1B4_5312 at 62.614 kb on + strand at 62.615 kb on - strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.635 kb on - strand, within Pf1N1B4_5312 at 62.725 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.827 kb on - strand, within Pf1N1B4_5312 at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.893 kb on - strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.971 kb on + strand at 62.971 kb on + strand at 62.972 kb on - strand at 63.131 kb on - strand, within Pf1N1B4_5313
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 61,074 + +1.1 61,075 - +0.1 61,098 - +0.8 61,222 - +0.1 61,421 - -0.5 61,421 - +1.7 61,448 - -1.5 61,464 + -0.7 61,496 + +0.3 61,534 + -1.2 61,605 - +0.1 61,675 + +0.4 61,675 + +0.7 61,676 - -1.1 61,679 + +0.1 61,679 + -0.4 61,679 + -0.7 61,679 + -1.0 61,679 + -0.1 61,679 + -0.5 61,679 + +0.9 61,679 + -1.4 61,679 + +0.8 61,680 - -0.9 61,714 + -1.9 61,714 + +0.1 61,723 + +2.1 61,866 + +1.2 61,920 + -0.7 61,920 + -1.9 61,920 + +0.5 61,920 + -1.2 61,920 + +1.3 61,920 + -0.2 61,920 + +0.3 61,920 + +1.5 61,920 + +0.7 61,920 + +1.2 61,920 + +1.1 61,920 + +0.2 61,920 + +0.6 61,920 + -0.8 61,920 + +0.8 61,921 - +1.4 61,921 - -0.8 61,921 - +1.1 62,067 + Pf1N1B4_5311 0.35 -1.2 62,068 - Pf1N1B4_5311 0.36 -1.1 62,113 + Pf1N1B4_5311 0.51 +0.6 62,113 + Pf1N1B4_5311 0.51 -1.2 62,279 + +0.8 62,279 + +1.7 62,279 + -1.2 62,279 + +0.1 62,323 + +0.6 62,323 + +0.6 62,323 + +0.0 62,323 + -1.2 62,324 - -1.2 62,324 - +1.6 62,324 - -0.4 62,324 - -1.1 62,329 + +0.4 62,330 + +0.1 62,330 + +1.1 62,331 - +1.4 62,372 + +0.7 62,387 + +0.4 62,387 + -0.5 62,387 + -0.0 62,387 + -0.9 62,387 + +2.8 62,387 + +0.9 62,387 + +0.5 62,387 + +0.5 62,387 + +0.3 62,387 + +0.3 62,388 - -0.1 62,388 - -1.4 62,388 - +0.5 62,421 + +0.8 62,421 + +1.1 62,421 + +1.1 62,421 + +2.1 62,430 + -1.2 62,456 + +0.1 62,456 + -0.2 62,457 - +0.3 62,614 + Pf1N1B4_5312 0.27 -0.3 62,614 + +2.1 62,615 - Pf1N1B4_5312 0.27 -1.1 62,634 + Pf1N1B4_5312 0.32 +0.2 62,634 + Pf1N1B4_5312 0.32 +1.4 62,635 - Pf1N1B4_5312 0.32 +1.0 62,725 + Pf1N1B4_5312 0.53 -0.9 62,826 + Pf1N1B4_5312 0.76 +1.1 62,826 + Pf1N1B4_5312 0.76 -0.9 62,827 - Pf1N1B4_5312 0.76 -1.4 62,892 + +0.7 62,892 + -0.7 62,892 + +0.5 62,892 + -0.4 62,893 - +1.1 62,952 + -1.2 62,952 + +0.9 62,952 + -0.2 62,968 + -1.7 62,968 + +0.6 62,968 + -1.7 62,968 + +0.5 62,971 + -0.5 62,971 + -0.7 62,972 - -0.9 63,131 - Pf1N1B4_5313 0.18 +0.3
Or see this region's nucleotide sequence