Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5014 and Pf1N1B4_5015 are separated by 129 nucleotides Pf1N1B4_5015 and Pf1N1B4_5016 are separated by 280 nucleotides Pf1N1B4_5016 and Pf1N1B4_5017 are separated by 140 nucleotides
Pf1N1B4_5014 - Glutathione reductase (EC 1.8.1.7), at 5,565,107 to 5,566,465
_5014
Pf1N1B4_5015 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 5,566,595 to 5,567,434
_5015
Pf1N1B4_5016 - hypothetical protein, at 5,567,715 to 5,567,903
_5016
Pf1N1B4_5017 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,568,044 to 5,568,325
_5017
Position (kb)
5566
5567
5568 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5565.719 kb on - strand, within Pf1N1B4_5014 at 5565.719 kb on - strand, within Pf1N1B4_5014 at 5565.940 kb on - strand, within Pf1N1B4_5014 at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.444 kb on - strand at 5566.444 kb on - strand at 5566.458 kb on + strand at 5566.493 kb on + strand at 5566.493 kb on + strand at 5566.528 kb on + strand at 5566.528 kb on + strand at 5566.529 kb on - strand at 5566.529 kb on - strand at 5566.529 kb on - strand at 5566.599 kb on + strand at 5566.653 kb on + strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.698 kb on + strand, within Pf1N1B4_5015 at 5566.926 kb on + strand, within Pf1N1B4_5015 at 5566.926 kb on + strand, within Pf1N1B4_5015 at 5566.927 kb on - strand at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.226 kb on + strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.437 kb on - strand at 5567.437 kb on - strand at 5567.437 kb on - strand at 5567.478 kb on + strand at 5567.498 kb on + strand at 5567.498 kb on + strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.499 kb on - strand at 5567.533 kb on - strand at 5567.552 kb on + strand at 5567.553 kb on - strand at 5567.581 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.651 kb on - strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.955 kb on + strand at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.238 kb on + strand, within Pf1N1B4_5017 at 5568.291 kb on - strand, within Pf1N1B4_5017 at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.324 kb on - strand at 5568.342 kb on - strand at 5568.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 5,565,719 - Pf1N1B4_5014 0.45 -0.8 5,565,719 - Pf1N1B4_5014 0.45 -1.1 5,565,940 - Pf1N1B4_5014 0.61 +0.9 5,566,443 + -0.8 5,566,443 + -0.8 5,566,443 + +0.8 5,566,443 + -0.1 5,566,443 + -1.2 5,566,443 + -0.5 5,566,444 - -0.8 5,566,444 - -1.8 5,566,458 + +1.2 5,566,493 + +2.5 5,566,493 + +0.3 5,566,528 + +2.5 5,566,528 + +1.4 5,566,529 - -0.3 5,566,529 - -2.3 5,566,529 - +1.1 5,566,599 + +1.5 5,566,653 + +0.5 5,566,654 - +0.1 5,566,654 - +1.5 5,566,654 - +2.5 5,566,654 - -1.5 5,566,698 + Pf1N1B4_5015 0.12 -0.5 5,566,926 + Pf1N1B4_5015 0.39 -0.3 5,566,926 + Pf1N1B4_5015 0.39 -2.1 5,566,927 - -0.1 5,566,990 - Pf1N1B4_5015 0.47 +0.5 5,566,990 - Pf1N1B4_5015 0.47 -1.7 5,566,990 - Pf1N1B4_5015 0.47 -1.4 5,567,140 - Pf1N1B4_5015 0.65 -1.5 5,567,140 - Pf1N1B4_5015 0.65 +0.1 5,567,185 - Pf1N1B4_5015 0.70 -0.2 5,567,185 - Pf1N1B4_5015 0.70 -0.3 5,567,226 + Pf1N1B4_5015 0.75 -0.6 5,567,227 - Pf1N1B4_5015 0.75 +1.1 5,567,227 - Pf1N1B4_5015 0.75 -1.8 5,567,434 + +0.3 5,567,434 + +0.1 5,567,434 + +1.8 5,567,436 + -0.8 5,567,436 + +0.7 5,567,436 + -1.4 5,567,437 - +0.9 5,567,437 - -1.1 5,567,437 - -0.8 5,567,478 + +0.6 5,567,498 + +1.5 5,567,498 + -1.1 5,567,499 - +1.5 5,567,499 - +0.5 5,567,499 - +1.9 5,567,499 - -2.0 5,567,499 - +2.5 5,567,533 - -0.8 5,567,552 + -1.7 5,567,553 - -0.4 5,567,581 + -0.4 5,567,583 + -1.0 5,567,583 + +0.1 5,567,583 + -0.2 5,567,584 - +0.1 5,567,584 - -0.5 5,567,584 - +0.7 5,567,651 - -2.3 5,567,712 + +1.5 5,567,712 + +0.5 5,567,712 + +0.5 5,567,713 - +1.3 5,567,713 - -0.8 5,567,713 - +0.2 5,567,713 - -1.5 5,567,955 + +0.5 5,568,110 + Pf1N1B4_5017 0.23 +0.1 5,568,110 + Pf1N1B4_5017 0.23 +0.1 5,568,238 + Pf1N1B4_5017 0.69 +0.8 5,568,291 - Pf1N1B4_5017 0.88 -1.1 5,568,323 + +0.5 5,568,323 + -1.4 5,568,323 + -1.3 5,568,323 + +0.5 5,568,324 - +2.1 5,568,342 - +0.5 5,568,361 - +0.2
Or see this region's nucleotide sequence