Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4975 and Pf1N1B4_4976 are separated by 145 nucleotides Pf1N1B4_4976 and Pf1N1B4_4977 are separated by 111 nucleotides
Pf1N1B4_4975 - hypothetical protein, at 5,514,804 to 5,515,145
_4975
Pf1N1B4_4976 - D-serine/D-alanine/glycine transporter, at 5,515,291 to 5,516,712
_4976
Pf1N1B4_4977 - Ornithine cyclodeaminase (EC 4.3.1.12), at 5,516,824 to 5,517,849
_4977
Position (kb)
5515
5516
5517 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5514.363 kb on + strand at 5514.558 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.680 kb on - strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.695 kb on - strand at 5514.695 kb on - strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.815 kb on - strand at 5514.815 kb on - strand at 5514.919 kb on - strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand at 5515.042 kb on - strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.160 kb on + strand at 5515.548 kb on - strand, within Pf1N1B4_4976 at 5515.572 kb on + strand, within Pf1N1B4_4976 at 5515.573 kb on - strand, within Pf1N1B4_4976 at 5515.767 kb on + strand, within Pf1N1B4_4976 at 5515.767 kb on + strand at 5516.038 kb on + strand, within Pf1N1B4_4976 at 5516.409 kb on - strand, within Pf1N1B4_4976 at 5516.492 kb on - strand, within Pf1N1B4_4976 at 5516.547 kb on + strand at 5516.549 kb on + strand, within Pf1N1B4_4976 at 5516.554 kb on - strand, within Pf1N1B4_4976 at 5516.557 kb on + strand, within Pf1N1B4_4976 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.186 kb on + strand, within Pf1N1B4_4977 at 5517.320 kb on + strand at 5517.321 kb on - strand, within Pf1N1B4_4977 at 5517.641 kb on + strand at 5517.641 kb on + strand, within Pf1N1B4_4977 at 5517.642 kb on - strand, within Pf1N1B4_4977 at 5517.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 5,514,363 + -0.3 5,514,558 + +0.9 5,514,580 + +0.9 5,514,580 + +2.8 5,514,580 + +0.5 5,514,580 + +2.5 5,514,580 + -1.8 5,514,580 + +1.5 5,514,679 + +1.9 5,514,679 + -0.4 5,514,679 + +1.1 5,514,679 + -0.0 5,514,679 + +1.3 5,514,679 + +0.6 5,514,680 - +0.2 5,514,691 + -0.1 5,514,691 + +3.1 5,514,691 + +0.1 5,514,691 + -0.4 5,514,695 - +0.0 5,514,695 - -1.1 5,514,814 + +1.1 5,514,814 + +1.2 5,514,814 + +2.1 5,514,815 - +0.2 5,514,815 - -0.8 5,514,919 - Pf1N1B4_4975 0.34 -1.0 5,515,041 + Pf1N1B4_4975 0.69 +0.6 5,515,041 + Pf1N1B4_4975 0.69 +0.7 5,515,041 + +1.1 5,515,042 - Pf1N1B4_4975 0.70 +1.1 5,515,074 + Pf1N1B4_4975 0.79 +1.2 5,515,074 + Pf1N1B4_4975 0.79 +0.1 5,515,074 + +1.5 5,515,074 + Pf1N1B4_4975 0.79 +2.2 5,515,160 + +1.5 5,515,548 - Pf1N1B4_4976 0.18 -0.8 5,515,572 + Pf1N1B4_4976 0.20 -1.0 5,515,573 - Pf1N1B4_4976 0.20 +0.1 5,515,767 + Pf1N1B4_4976 0.33 +0.3 5,515,767 + -1.8 5,516,038 + Pf1N1B4_4976 0.53 -1.5 5,516,409 - Pf1N1B4_4976 0.79 -1.5 5,516,492 - Pf1N1B4_4976 0.84 -1.2 5,516,547 + -0.1 5,516,549 + Pf1N1B4_4976 0.88 +0.5 5,516,554 - Pf1N1B4_4976 0.89 -0.5 5,516,557 + Pf1N1B4_4976 0.89 +0.0 5,517,038 + Pf1N1B4_4977 0.21 -0.3 5,517,038 + Pf1N1B4_4977 0.21 -1.0 5,517,186 + Pf1N1B4_4977 0.35 +0.9 5,517,320 + +0.5 5,517,321 - Pf1N1B4_4977 0.48 +1.1 5,517,641 + +2.1 5,517,641 + Pf1N1B4_4977 0.80 -0.6 5,517,642 - Pf1N1B4_4977 0.80 -1.1 5,517,654 - -0.5
Or see this region's nucleotide sequence