Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4947 and Pf1N1B4_4948 are separated by 0 nucleotides Pf1N1B4_4948 and Pf1N1B4_4949 overlap by 4 nucleotides Pf1N1B4_4949 and Pf1N1B4_4950 are separated by 4 nucleotides
Pf1N1B4_4947 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 5,481,979 to 5,485,044
_4947
Pf1N1B4_4948 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 5,485,045 to 5,485,989
_4948
Pf1N1B4_4949 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 5,485,986 to 5,486,639
_4949
Pf1N1B4_4950 - formate dehydrogenase formation protein FdhE, at 5,486,644 to 5,487,570
_4950
Position (kb) 5485
5486
5487 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5485.043 kb on - strand at 5485.226 kb on - strand, within Pf1N1B4_4948 at 5485.254 kb on + strand at 5485.254 kb on + strand at 5485.504 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.506 kb on + strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.507 kb on - strand, within Pf1N1B4_4948 at 5485.629 kb on + strand, within Pf1N1B4_4948 at 5485.670 kb on + strand, within Pf1N1B4_4948 at 5485.671 kb on - strand, within Pf1N1B4_4948 at 5485.672 kb on + strand, within Pf1N1B4_4948 at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.673 kb on - strand at 5485.673 kb on - strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand, within Pf1N1B4_4948 at 5485.708 kb on + strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.709 kb on - strand at 5485.709 kb on - strand at 5485.709 kb on - strand, within Pf1N1B4_4948 at 5485.858 kb on + strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5485.859 kb on - strand, within Pf1N1B4_4948 at 5486.011 kb on - strand at 5486.011 kb on - strand at 5486.157 kb on + strand at 5486.157 kb on + strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.158 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.221 kb on - strand, within Pf1N1B4_4949 at 5486.307 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.493 kb on + strand, within Pf1N1B4_4949 at 5486.672 kb on + strand at 5486.672 kb on + strand at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5486.783 kb on - strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.247 kb on + strand, within Pf1N1B4_4950 at 5487.319 kb on + strand, within Pf1N1B4_4950 at 5487.346 kb on + strand, within Pf1N1B4_4950 at 5487.347 kb on - strand, within Pf1N1B4_4950 at 5487.425 kb on - strand, within Pf1N1B4_4950 at 5487.580 kb on + strand at 5487.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 5,485,043 - +1.5 5,485,226 - Pf1N1B4_4948 0.19 +0.8 5,485,254 + +1.5 5,485,254 + +0.1 5,485,504 + Pf1N1B4_4948 0.49 +0.2 5,485,506 + Pf1N1B4_4948 0.49 +0.5 5,485,506 + Pf1N1B4_4948 0.49 -0.1 5,485,506 + Pf1N1B4_4948 0.49 -1.9 5,485,507 - Pf1N1B4_4948 0.49 -0.5 5,485,507 - Pf1N1B4_4948 0.49 +0.0 5,485,507 - Pf1N1B4_4948 0.49 -1.7 5,485,629 + Pf1N1B4_4948 0.62 +0.0 5,485,670 + Pf1N1B4_4948 0.66 -0.5 5,485,671 - Pf1N1B4_4948 0.66 -1.5 5,485,672 + Pf1N1B4_4948 0.66 +2.1 5,485,673 - Pf1N1B4_4948 0.66 +0.2 5,485,673 - +0.5 5,485,673 - Pf1N1B4_4948 0.66 -0.5 5,485,708 + Pf1N1B4_4948 0.70 -0.5 5,485,708 + Pf1N1B4_4948 0.70 -1.1 5,485,708 + Pf1N1B4_4948 0.70 +1.2 5,485,708 + Pf1N1B4_4948 0.70 +0.9 5,485,708 + Pf1N1B4_4948 0.70 +0.7 5,485,708 + -0.3 5,485,709 - Pf1N1B4_4948 0.70 -0.5 5,485,709 - -0.5 5,485,709 - -0.5 5,485,709 - Pf1N1B4_4948 0.70 -0.9 5,485,858 + Pf1N1B4_4948 0.86 -0.4 5,485,859 - Pf1N1B4_4948 0.86 -0.9 5,485,859 - Pf1N1B4_4948 0.86 -1.1 5,485,859 - Pf1N1B4_4948 0.86 -0.5 5,486,011 - -0.1 5,486,011 - -0.3 5,486,157 + -0.1 5,486,157 + Pf1N1B4_4949 0.26 +0.9 5,486,158 - Pf1N1B4_4949 0.26 +0.5 5,486,158 - Pf1N1B4_4949 0.26 -1.1 5,486,221 - Pf1N1B4_4949 0.36 -0.5 5,486,221 - Pf1N1B4_4949 0.36 -0.7 5,486,221 - Pf1N1B4_4949 0.36 -1.5 5,486,307 + Pf1N1B4_4949 0.49 -0.1 5,486,493 + Pf1N1B4_4949 0.78 +1.1 5,486,493 + Pf1N1B4_4949 0.78 -1.3 5,486,493 + Pf1N1B4_4949 0.78 -1.6 5,486,672 + +0.1 5,486,672 + +1.5 5,486,783 - Pf1N1B4_4950 0.15 -1.5 5,486,783 - Pf1N1B4_4950 0.15 +1.2 5,487,247 + Pf1N1B4_4950 0.65 -0.0 5,487,247 + Pf1N1B4_4950 0.65 -0.9 5,487,319 + Pf1N1B4_4950 0.73 +1.1 5,487,346 + Pf1N1B4_4950 0.76 -0.1 5,487,347 - Pf1N1B4_4950 0.76 +1.5 5,487,425 - Pf1N1B4_4950 0.84 -0.5 5,487,580 + -1.5 5,487,627 + +0.8
Or see this region's nucleotide sequence