Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4647

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4646 and Pf1N1B4_4647 are separated by 205 nucleotidesPf1N1B4_4647 and Pf1N1B4_4648 are separated by 228 nucleotides Pf1N1B4_4646 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 5,125,168 to 5,126,601 _4646 Pf1N1B4_4647 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 5,126,807 to 5,127,247 _4647 Pf1N1B4_4648 - transcriptional regulator, MerR family, at 5,127,476 to 5,128,453 _4648 Position (kb) 5126 5127 5128Strain fitness (log2 ratio) -1 0 1at 5125.873 kb on - strand, within Pf1N1B4_4646at 5126.263 kb on + strand, within Pf1N1B4_4646at 5126.378 kb on + strand, within Pf1N1B4_4646at 5126.432 kb on + strand, within Pf1N1B4_4646at 5126.433 kb on - strand, within Pf1N1B4_4646at 5126.562 kb on + strandat 5126.769 kb on + strandat 5126.785 kb on - strandat 5126.826 kb on - strandat 5127.246 kb on - strandat 5127.550 kb on - strandat 5127.914 kb on - strand, within Pf1N1B4_4648at 5128.046 kb on + strand, within Pf1N1B4_4648at 5128.220 kb on + strand, within Pf1N1B4_4648at 5128.220 kb on + strand, within Pf1N1B4_4648

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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5,125,873 - Pf1N1B4_4646 0.49 -0.1
5,126,263 + Pf1N1B4_4646 0.76 -0.5
5,126,378 + Pf1N1B4_4646 0.84 -0.6
5,126,432 + Pf1N1B4_4646 0.88 +1.1
5,126,433 - Pf1N1B4_4646 0.88 +0.3
5,126,562 + +0.5
5,126,769 + +0.1
5,126,785 - +0.1
5,126,826 - -1.5
5,127,246 - +0.5
5,127,550 - +0.3
5,127,914 - Pf1N1B4_4648 0.45 +1.1
5,128,046 + Pf1N1B4_4648 0.58 +0.8
5,128,220 + Pf1N1B4_4648 0.76 +0.5
5,128,220 + Pf1N1B4_4648 0.76 +0.7

Or see this region's nucleotide sequence