Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_456

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_453 and Pf1N1B4_454 are separated by 34 nucleotidesPf1N1B4_454 and Pf1N1B4_455 are separated by 13 nucleotidesPf1N1B4_455 and Pf1N1B4_456 are separated by 104 nucleotidesPf1N1B4_456 and Pf1N1B4_457 are separated by 73 nucleotides Pf1N1B4_453 - Phosphate:acyl-ACP acyltransferase PlsX, at 544,633 to 545,613 _453 Pf1N1B4_454 - LSU ribosomal protein L32p, at 545,648 to 545,830 _454 Pf1N1B4_455 - COG1399 protein, clustered with ribosomal protein L32p, at 545,844 to 546,371 _455 Pf1N1B4_456 - FIG146278: Maf/YceF/YhdE family protein, at 546,476 to 547,054 _456 Pf1N1B4_457 - Periplasmic serine proteases (ClpP class), at 547,128 to 548,144 _457 Position (kb) 546 547 548Strain fitness (log2 ratio) -2 -1 0 1 2at 545.554 kb on - strandat 546.486 kb on + strandat 546.487 kb on - strandat 546.487 kb on - strandat 546.489 kb on + strandat 546.490 kb on - strandat 546.609 kb on + strand, within Pf1N1B4_456at 546.610 kb on - strandat 546.750 kb on + strand, within Pf1N1B4_456at 546.750 kb on + strand, within Pf1N1B4_456at 546.750 kb on + strand, within Pf1N1B4_456at 546.750 kb on + strand, within Pf1N1B4_456at 546.751 kb on - strand, within Pf1N1B4_456at 546.751 kb on - strand, within Pf1N1B4_456at 546.751 kb on - strand, within Pf1N1B4_456at 546.751 kb on - strand, within Pf1N1B4_456at 546.751 kb on - strand, within Pf1N1B4_456at 546.849 kb on + strand, within Pf1N1B4_456at 546.850 kb on - strand, within Pf1N1B4_456at 546.888 kb on - strand, within Pf1N1B4_456at 546.908 kb on + strand, within Pf1N1B4_456at 546.908 kb on + strand, within Pf1N1B4_456at 547.020 kb on + strandat 547.022 kb on - strandat 547.022 kb on - strandat 547.022 kb on - strandat 547.048 kb on - strandat 547.404 kb on - strand, within Pf1N1B4_457at 547.513 kb on + strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.514 kb on - strand, within Pf1N1B4_457at 547.518 kb on - strand, within Pf1N1B4_457at 547.612 kb on + strand, within Pf1N1B4_457at 547.613 kb on - strand, within Pf1N1B4_457at 547.639 kb on + strandat 547.639 kb on + strand, within Pf1N1B4_457at 547.639 kb on + strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.640 kb on - strand, within Pf1N1B4_457at 547.642 kb on + strand, within Pf1N1B4_457at 547.642 kb on + strandat 547.643 kb on - strand, within Pf1N1B4_457at 547.688 kb on + strandat 547.689 kb on - strand, within Pf1N1B4_457at 547.733 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.873 kb on - strand, within Pf1N1B4_457at 547.874 kb on + strand, within Pf1N1B4_457at 547.875 kb on - strand, within Pf1N1B4_457at 547.927 kb on - strand, within Pf1N1B4_457at 547.927 kb on - strand, within Pf1N1B4_457at 547.964 kb on - strand, within Pf1N1B4_457at 548.050 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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545,554 - +0.4
546,486 + +1.7
546,487 - +0.9
546,487 - +0.2
546,489 + +0.9
546,490 - +1.2
546,609 + Pf1N1B4_456 0.23 -0.9
546,610 - +0.6
546,750 + Pf1N1B4_456 0.47 -0.7
546,750 + Pf1N1B4_456 0.47 -1.0
546,750 + Pf1N1B4_456 0.47 -0.3
546,750 + Pf1N1B4_456 0.47 -0.1
546,751 - Pf1N1B4_456 0.47 -0.7
546,751 - Pf1N1B4_456 0.47 -0.9
546,751 - Pf1N1B4_456 0.47 -0.1
546,751 - Pf1N1B4_456 0.47 -0.9
546,751 - Pf1N1B4_456 0.47 -0.4
546,849 + Pf1N1B4_456 0.64 +0.0
546,850 - Pf1N1B4_456 0.65 -0.4
546,888 - Pf1N1B4_456 0.71 +0.0
546,908 + Pf1N1B4_456 0.75 -1.4
546,908 + Pf1N1B4_456 0.75 -0.2
547,020 + -0.7
547,022 - -1.7
547,022 - +0.2
547,022 - -0.7
547,048 - +0.6
547,404 - Pf1N1B4_457 0.27 -1.4
547,513 + Pf1N1B4_457 0.38 +0.9
547,514 - Pf1N1B4_457 0.38 -2.1
547,514 - Pf1N1B4_457 0.38 -1.1
547,514 - Pf1N1B4_457 0.38 -0.2
547,514 - Pf1N1B4_457 0.38 -1.7
547,514 - Pf1N1B4_457 0.38 -0.7
547,514 - Pf1N1B4_457 0.38 +1.3
547,518 - Pf1N1B4_457 0.38 -0.7
547,612 + Pf1N1B4_457 0.48 +0.2
547,613 - Pf1N1B4_457 0.48 +0.2
547,639 + +1.9
547,639 + Pf1N1B4_457 0.50 -0.4
547,639 + Pf1N1B4_457 0.50 -1.7
547,640 - Pf1N1B4_457 0.50 +1.5
547,640 - Pf1N1B4_457 0.50 -0.6
547,640 - Pf1N1B4_457 0.50 -0.1
547,640 - Pf1N1B4_457 0.50 -0.7
547,640 - Pf1N1B4_457 0.50 +0.2
547,640 - Pf1N1B4_457 0.50 +0.9
547,640 - Pf1N1B4_457 0.50 +0.6
547,640 - Pf1N1B4_457 0.50 -0.1
547,640 - Pf1N1B4_457 0.50 -0.1
547,640 - Pf1N1B4_457 0.50 +0.5
547,642 + Pf1N1B4_457 0.51 +0.3
547,642 + +0.9
547,643 - Pf1N1B4_457 0.51 -0.3
547,688 + +0.9
547,689 - Pf1N1B4_457 0.55 -1.5
547,733 - Pf1N1B4_457 0.59 +0.7
547,873 - Pf1N1B4_457 0.73 -0.8
547,873 - Pf1N1B4_457 0.73 +0.4
547,873 - Pf1N1B4_457 0.73 +1.2
547,874 + Pf1N1B4_457 0.73 +0.9
547,875 - Pf1N1B4_457 0.73 -0.9
547,927 - Pf1N1B4_457 0.79 +2.1
547,927 - Pf1N1B4_457 0.79 +0.0
547,964 - Pf1N1B4_457 0.82 -0.4
548,050 - -1.1

Or see this region's nucleotide sequence