Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4423

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4422 and Pf1N1B4_4423 are separated by 64 nucleotidesPf1N1B4_4423 and Pf1N1B4_4424 overlap by 4 nucleotides Pf1N1B4_4422 - ABC transporter amino acid-binding protein, at 4,882,588 to 4,883,430 _4422 Pf1N1B4_4423 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 4,883,495 to 4,884,346 _4423 Pf1N1B4_4424 - D-2-hydroxyglutarate dehydrogenase, at 4,884,343 to 4,885,767 _4424 Position (kb) 4883 4884 4885Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4882.730 kb on + strand, within Pf1N1B4_4422at 4882.731 kb on - strand, within Pf1N1B4_4422at 4882.967 kb on - strand, within Pf1N1B4_4422at 4883.066 kb on - strand, within Pf1N1B4_4422at 4883.850 kb on - strand, within Pf1N1B4_4423at 4884.091 kb on + strand, within Pf1N1B4_4423at 4884.091 kb on + strand, within Pf1N1B4_4423at 4884.091 kb on + strand, within Pf1N1B4_4423at 4884.092 kb on - strand, within Pf1N1B4_4423at 4884.092 kb on - strand, within Pf1N1B4_4423at 4884.092 kb on - strand, within Pf1N1B4_4423at 4884.092 kb on - strand, within Pf1N1B4_4423at 4884.092 kb on - strand, within Pf1N1B4_4423at 4884.445 kb on + strandat 4884.445 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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4,882,730 + Pf1N1B4_4422 0.17 +1.4
4,882,731 - Pf1N1B4_4422 0.17 -2.8
4,882,967 - Pf1N1B4_4422 0.45 +0.1
4,883,066 - Pf1N1B4_4422 0.57 -0.4
4,883,850 - Pf1N1B4_4423 0.42 -0.2
4,884,091 + Pf1N1B4_4423 0.70 +1.3
4,884,091 + Pf1N1B4_4423 0.70 +2.6
4,884,091 + Pf1N1B4_4423 0.70 -0.4
4,884,092 - Pf1N1B4_4423 0.70 -1.4
4,884,092 - Pf1N1B4_4423 0.70 -0.9
4,884,092 - Pf1N1B4_4423 0.70 -1.8
4,884,092 - Pf1N1B4_4423 0.70 -0.9
4,884,092 - Pf1N1B4_4423 0.70 +0.1
4,884,445 + +0.6
4,884,445 + +1.0

Or see this region's nucleotide sequence