Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4386 and Pf1N1B4_4387 are separated by 162 nucleotides Pf1N1B4_4387 and Pf1N1B4_4388 overlap by 4 nucleotides Pf1N1B4_4388 and Pf1N1B4_4389 are separated by 200 nucleotides
Pf1N1B4_4386 - Inositol transport system sugar-binding protein, at 4,844,516 to 4,845,442
_4386
Pf1N1B4_4387 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,845,605 to 4,846,393
_4387
Pf1N1B4_4388 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,846,390 to 4,847,412
_4388
Pf1N1B4_4389 - Sigma-54 dependent transcriptional regulator, at 4,847,613 to 4,849,634
_4389
Position (kb)
4846
4847
4848 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4845.408 kb on - strand at 4845.463 kb on + strand at 4845.510 kb on + strand at 4845.511 kb on - strand at 4845.541 kb on - strand at 4845.570 kb on + strand at 4845.571 kb on - strand at 4845.571 kb on - strand at 4845.956 kb on - strand, within Pf1N1B4_4387 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.547 kb on + strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.548 kb on - strand, within Pf1N1B4_4388 at 4846.694 kb on + strand, within Pf1N1B4_4388 at 4846.695 kb on - strand at 4846.787 kb on + strand, within Pf1N1B4_4388 at 4846.787 kb on + strand, within Pf1N1B4_4388 at 4846.788 kb on - strand, within Pf1N1B4_4388 at 4847.240 kb on + strand, within Pf1N1B4_4388 at 4847.240 kb on + strand, within Pf1N1B4_4388 at 4847.406 kb on + strand at 4847.406 kb on + strand at 4847.406 kb on + strand at 4847.407 kb on - strand at 4847.407 kb on - strand at 4847.428 kb on + strand at 4847.428 kb on + strand at 4847.429 kb on - strand at 4847.429 kb on - strand at 4847.429 kb on - strand at 4847.584 kb on + strand at 4847.597 kb on + strand at 4847.597 kb on + strand at 4847.629 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.658 kb on + strand at 4847.659 kb on - strand at 4847.659 kb on - strand at 4847.659 kb on - strand at 4847.929 kb on - strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.081 kb on + strand, within Pf1N1B4_4389 at 4848.221 kb on + strand, within Pf1N1B4_4389 at 4848.221 kb on + strand, within Pf1N1B4_4389
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 4,845,408 - -1.4 4,845,463 + +0.6 4,845,510 + -0.2 4,845,511 - +0.9 4,845,541 - +0.9 4,845,570 + -1.2 4,845,571 - +1.3 4,845,571 - -0.6 4,845,956 - Pf1N1B4_4387 0.44 +0.6 4,846,547 + Pf1N1B4_4388 0.15 -0.6 4,846,547 + Pf1N1B4_4388 0.15 -0.8 4,846,547 + Pf1N1B4_4388 0.15 -1.4 4,846,548 - Pf1N1B4_4388 0.15 -1.0 4,846,548 - Pf1N1B4_4388 0.15 -0.6 4,846,548 - Pf1N1B4_4388 0.15 +0.6 4,846,694 + Pf1N1B4_4388 0.30 -0.1 4,846,695 - -0.0 4,846,787 + Pf1N1B4_4388 0.39 -1.0 4,846,787 + Pf1N1B4_4388 0.39 -3.0 4,846,788 - Pf1N1B4_4388 0.39 -0.0 4,847,240 + Pf1N1B4_4388 0.83 +0.3 4,847,240 + Pf1N1B4_4388 0.83 -0.2 4,847,406 + -0.1 4,847,406 + -2.0 4,847,406 + +0.2 4,847,407 - -1.1 4,847,407 - -0.3 4,847,428 + -0.2 4,847,428 + -0.1 4,847,429 - -0.0 4,847,429 - -0.1 4,847,429 - +0.3 4,847,584 + -1.6 4,847,597 + +1.2 4,847,597 + +1.1 4,847,629 + -1.4 4,847,658 + +0.6 4,847,658 + -0.1 4,847,658 + +1.6 4,847,658 + +0.2 4,847,658 + -0.1 4,847,659 - -1.0 4,847,659 - -0.4 4,847,659 - +0.6 4,847,929 - Pf1N1B4_4389 0.16 +1.6 4,848,081 + Pf1N1B4_4389 0.23 -0.4 4,848,081 + Pf1N1B4_4389 0.23 -1.0 4,848,081 + Pf1N1B4_4389 0.23 -0.6 4,848,081 + Pf1N1B4_4389 0.23 -0.1 4,848,081 + Pf1N1B4_4389 0.23 +1.6 4,848,081 + Pf1N1B4_4389 0.23 -0.0 4,848,081 + Pf1N1B4_4389 0.23 -0.9 4,848,221 + Pf1N1B4_4389 0.30 -1.0 4,848,221 + Pf1N1B4_4389 0.30 -1.2
Or see this region's nucleotide sequence