Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4388

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4386 and Pf1N1B4_4387 are separated by 162 nucleotidesPf1N1B4_4387 and Pf1N1B4_4388 overlap by 4 nucleotidesPf1N1B4_4388 and Pf1N1B4_4389 are separated by 200 nucleotides Pf1N1B4_4386 - Inositol transport system sugar-binding protein, at 4,844,516 to 4,845,442 _4386 Pf1N1B4_4387 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,845,605 to 4,846,393 _4387 Pf1N1B4_4388 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,846,390 to 4,847,412 _4388 Pf1N1B4_4389 - Sigma-54 dependent transcriptional regulator, at 4,847,613 to 4,849,634 _4389 Position (kb) 4846 4847 4848Strain fitness (log2 ratio) -3 -2 -1 0 1at 4845.408 kb on - strandat 4845.463 kb on + strandat 4845.510 kb on + strandat 4845.511 kb on - strandat 4845.541 kb on - strandat 4845.570 kb on + strandat 4845.571 kb on - strandat 4845.571 kb on - strandat 4845.956 kb on - strand, within Pf1N1B4_4387at 4846.547 kb on + strand, within Pf1N1B4_4388at 4846.547 kb on + strand, within Pf1N1B4_4388at 4846.547 kb on + strand, within Pf1N1B4_4388at 4846.548 kb on - strand, within Pf1N1B4_4388at 4846.548 kb on - strand, within Pf1N1B4_4388at 4846.548 kb on - strand, within Pf1N1B4_4388at 4846.694 kb on + strand, within Pf1N1B4_4388at 4846.695 kb on - strandat 4846.787 kb on + strand, within Pf1N1B4_4388at 4846.787 kb on + strand, within Pf1N1B4_4388at 4846.788 kb on - strand, within Pf1N1B4_4388at 4847.240 kb on + strand, within Pf1N1B4_4388at 4847.240 kb on + strand, within Pf1N1B4_4388at 4847.406 kb on + strandat 4847.406 kb on + strandat 4847.406 kb on + strandat 4847.407 kb on - strandat 4847.407 kb on - strandat 4847.428 kb on + strandat 4847.428 kb on + strandat 4847.429 kb on - strandat 4847.429 kb on - strandat 4847.429 kb on - strandat 4847.584 kb on + strandat 4847.597 kb on + strandat 4847.597 kb on + strandat 4847.629 kb on + strandat 4847.658 kb on + strandat 4847.658 kb on + strandat 4847.658 kb on + strandat 4847.658 kb on + strandat 4847.658 kb on + strandat 4847.659 kb on - strandat 4847.659 kb on - strandat 4847.659 kb on - strandat 4847.929 kb on - strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.081 kb on + strand, within Pf1N1B4_4389at 4848.221 kb on + strand, within Pf1N1B4_4389at 4848.221 kb on + strand, within Pf1N1B4_4389

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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4,845,408 - -1.4
4,845,463 + +0.6
4,845,510 + -0.2
4,845,511 - +0.9
4,845,541 - +0.9
4,845,570 + -1.2
4,845,571 - +1.3
4,845,571 - -0.6
4,845,956 - Pf1N1B4_4387 0.44 +0.6
4,846,547 + Pf1N1B4_4388 0.15 -0.6
4,846,547 + Pf1N1B4_4388 0.15 -0.8
4,846,547 + Pf1N1B4_4388 0.15 -1.4
4,846,548 - Pf1N1B4_4388 0.15 -1.0
4,846,548 - Pf1N1B4_4388 0.15 -0.6
4,846,548 - Pf1N1B4_4388 0.15 +0.6
4,846,694 + Pf1N1B4_4388 0.30 -0.1
4,846,695 - -0.0
4,846,787 + Pf1N1B4_4388 0.39 -1.0
4,846,787 + Pf1N1B4_4388 0.39 -3.0
4,846,788 - Pf1N1B4_4388 0.39 -0.0
4,847,240 + Pf1N1B4_4388 0.83 +0.3
4,847,240 + Pf1N1B4_4388 0.83 -0.2
4,847,406 + -0.1
4,847,406 + -2.0
4,847,406 + +0.2
4,847,407 - -1.1
4,847,407 - -0.3
4,847,428 + -0.2
4,847,428 + -0.1
4,847,429 - -0.0
4,847,429 - -0.1
4,847,429 - +0.3
4,847,584 + -1.6
4,847,597 + +1.2
4,847,597 + +1.1
4,847,629 + -1.4
4,847,658 + +0.6
4,847,658 + -0.1
4,847,658 + +1.6
4,847,658 + +0.2
4,847,658 + -0.1
4,847,659 - -1.0
4,847,659 - -0.4
4,847,659 - +0.6
4,847,929 - Pf1N1B4_4389 0.16 +1.6
4,848,081 + Pf1N1B4_4389 0.23 -0.4
4,848,081 + Pf1N1B4_4389 0.23 -1.0
4,848,081 + Pf1N1B4_4389 0.23 -0.6
4,848,081 + Pf1N1B4_4389 0.23 -0.1
4,848,081 + Pf1N1B4_4389 0.23 +1.6
4,848,081 + Pf1N1B4_4389 0.23 -0.0
4,848,081 + Pf1N1B4_4389 0.23 -0.9
4,848,221 + Pf1N1B4_4389 0.30 -1.0
4,848,221 + Pf1N1B4_4389 0.30 -1.2

Or see this region's nucleotide sequence